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Charakterystyka celulolitycznego szczepu Micromonospora rubra wyizolowanego z gleby

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Anerek B., Gorska E. B., Russel S.

Acta Agraria et Silvestria. Series Agraria

2006

tom 49

In present paper the cellulolityc activity of soil strain Micromonospora rubra was investigated. The strain was incubated in Dubois medium amended with 1% filter paper, carboxymethyl- cellulose or microcrystalline Avicel cellulose as the single carbon source. The above cultures were incubated at 28"C on rotary shaker for 10 days. The biosynthesis of cellulolytic enzymes (FP-ase, CMC-ase) was determined using colorimetric method suggested by International Biochemical Committee. The activity of cellulolytic enzymes was measured in 2 days intervals in proper supernatants. The effect of pH on FP-ase and CMC-ase activity was determined using supernatant obtained by centrifugation of 10 days old culture of investigated Mi- cromonospora strain in medium amended with CMC. The investigated strain M. rubra was able to produce both FP-ase and CMC-ase. The intensity of above enzymes depended from kind of cellulosic substrate and incubation time of culture. The highest activity of both enzymes was obtained using CMC as a carbon source. The investigated M. rubra strain produced only trace amounts of FP-ase and CMC-ase in medium amended with filter paper. Optimum pH of both enzymes was 6.0.

Ograniczanie wyników

1 Acta Agraria et Silvestria. Series Agraria

1 Anerek B.

1 Gorska E. B.

1 Russel S.

1 2006

Wyniki wyszukiwania

Charakterystyka celulolitycznego szczepu Micromonospora rubra wyizolowanego z gleby