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1

Wpływ warunków przechowywania nasion rzepaku na zawartość wybranych mikotoksyn alternaryjnych

100%
Gwiazdowski R., Wickiel G.
Zeszyty Problemowe Postępów Nauk Rolniczych
|
2010
|
tom 556
|
nr 1
W pracy oceniono wpływ warunków przechowywania na zawartość wybranych mikotoksyn alternaryjnych w nasionach rzepaku. Analiza mikologiczna po zbiorze wykazała obecność grzybów z rodzaju Alternaria w nasionach rzepaku zarówno w odmianach jarych, jak i ozimych. Mikotoksyny AOH i AME występowały w niskich ilościach lub były niewykrywalne. Podczas przechowywania ilość obu toksyn znacznie wzrastała. W warunkach podwyższonej aktywności wodnej obserwowano duży przyrost AOH. Ilość AME była porównywalna niezależnie od warunków przechowywania.
2

Zasiedlenie ziarna pszenicy ozimej orkisz przez grzyby

100%
Filoda G., Wickiel G.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 2
The aim of this work was to determine the occurrence of fungi on grain of winter spelt cultivated in Głubczyce (South of Poland) and in Winna Góra (West-Central Poland) with special attention on toxigenic species. Mycological analysis of grain healthiness was performed after 8 weeks post harvest. Additionally grain after grinding was analyzed for the presence of deoksynivalenol (DON) and nivalenol (NIV) using HPLC. Among pathogenic species Fusarium avenaceum dominated whereas saprotrophic fungi were represented mainly by Alternaria alternata in the case of both locations. Based on the results of mycotoxin determination it was concluded that grain of winter spelt contained only trace amounts of trichothecenes.
3

Wpływ stosowania fungicydu na ograniczenie w nasionach rzepaku ozimego zawartości toksyn grzybów z rodzaju Alternaria

100%
Gwiazdowski R., Wickiel G.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 3
In the presented paper six rape cultivars in the season 2010, were tested for the presence of mycotoxins with HPLC methods. HPLC analyses were preceded by the evaluation of pods’ health on the plots and mycological analysis after harvest. Fungicide application significantly reduced the number of fungi isolated after harvest. A low or very low level of alternariol and alternariol methyl ether was found in seeds of oilseed rape both from treated and untreated combinations. Fungicide application reduced the content of alternariol and alternariol methyl ether in seeds of majority of cultivars.
4

Występowanie fuzariozy kolb kukurydzy (Fusarium spp.) oraz zawartość mikotoksyn w ziarnie kukurydzy chronionej zabiegiem fungicydowym

100%
Wickiel G., Lawecki T.
Progress in Plant Protection
|
2009
|
tom 49
|
nr 4
5

Fungicide protection and presence of fusarium ear symptoms and deoxynivalenol content in the grain of winter spelt

100%
Wickiel G., Filoda G.
Progress in Plant Protection
|
2012
|
tom 52
|
nr 3
Fusarium head blight (FHB) is perceived as a threat to wheat production. Besides the reduction of the yield potential of infected plants, the fungus contaminates kernels with different mycotoxins. The objective of our studies was to follow the development of Fusarium culmorum of FHB pathogen in winter spelt. Two fungicide experiments with and without inoculation was conducted in Winna Góra. F. culmorum was isolated from winter wheat ears with significally disease symptoms in 2009. The most aggressive isolate was used for inoculation of spelt in 2011. Colonisation of ears by F. culmorum was low in both experiments and no other Fusarium species could be detected. The used fungicides reduced significantly the level of infection but the concentration of deoxynivalenol (DON) in the grain was not correlated with Fusarium occurrence on ears and grain.
6

Porażenie kłosów pszenicy ozimej przez grzyby z rodzaju Fusarium a ryzyko występowania mikotoksyn w ziarnie

100%
Filoda G., Wickiel G.
Progress in Plant Protection
|
2009
|
tom 49
|
nr 2
7

Występowanie mikotoksyn alternaryjnych w nasionach rzepaku w zależności od odmian

100%
Gwiazdowski R., Wickiel G.
Progress in Plant Protection
|
2009
|
tom 49
|
nr 2
8

Występowanie mikotoksyn w ziarnie różnych odmian owsa

81%
Wickiel G., Bednarek-Bartsch A., Wower K., Kardasz P.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 3
During the growing season, oats is exposed to the pathogenic filamentous fungi, among others of Fusarium spp., which in favorable conditions, may secrete secondary metabolites called mycotoxins that are harmful to the consumer. Grains of five cultivars of naturally contaminated oats were analyzed for Fusarium infection level and mycotoxins (NIV, DON, T-2 and HT-2 toxins) accumulation. The predominant Fusarium species was a common oats pathogen: F. poae (Peck) Wollenw. Among the other identified Fusarium species were F. equiseti (Corda) Sacc. i F. oxysporum Schlecht. emend. Snyd et Hans. The Fusarium fungi infection level of grain was very low for all cultivars and ranged from 2 to 3%. The level of mycotoxins detected in the grain samples was also generally low and ranged from respectively: 53 μg/kg (ppb) for nivalenol, < LOQ for deoxynivalenol and < LOQ for T-2 and HT-2 toxins in the grain of Gniady cv. to respectively: 142 μg/kg (ppb) for nivalenol, < LOQ for deoxynivalenol and 38 μg/kg (ppb) for T-2 and HT-2 toxins in the grain of Krezus cv.
9

Influence of fungicide application on limitation of fungi on oilseed rape siliques and seeds

81%
Jajor E., Horoszkiewicz-Janka J., Wickiel G.
Progress in Plant Protection
|
2013
|
tom 53
|
nr 4
The aim of the study was to assess the influence of fungicidal protection on limitation of rape siliques and seeds infection by fungi. The controlled experiments were conducted on rape winter and spring rape cultivars at the Field Experimental Station of the Institute of Plant Protection – National Research Institute in Winna Góra for three years. Fungicide treatments were carried out at the budding stage of rape and at the end of rape flowering. Percentage of silique surface infected was determined during ripening. After harvesting, yield parameters, seed colonization by fungi and mycotoxin content were assessed. In the conducted experiments, symptoms of Alternaria blight on siliques were mainly observed. Higher degree of silique infection and seed colonization by fungi from the genus Alternaria was noted in spring rape. Effectiveness of reduction of Alternaria blight infection by fungicides depended on analyzed season, cultivar and time of use of plant protection products. Oilseed rape yield of both cultivars was significantly and negatively correlated with infected rate of siliques. Seed colonization by fungi of the genus Alternaria was the highest in control and it was confirmed mainly in cultivar Licosmos. The conducted chromatographic analyses enabled to find small amounts of alternariol monomethyl ether and alternariol in rape seeds.
10

Grzyby rodzaju Alternaria i ich toksyczne metabolity występujące w nasionach rzepaku ozimego

81%
Jajor E., Wickiel G., Horoszkiewicz-Janka J.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 4
The aim of this study was the evaluation of the oilseed rape colonisation by Alternaria genus fungi and the determination of mycotoxins produced by them. The seeds of Lisek cultivar were collected from untreated plants and four objects which were treated at the budding stage (BBCH 58–59) and at the late flowering stage (BBCH 68–69) with fungicides Amistar 250 SC and Caramba 60 SL. After harvesting, the seeds were evaluated for the colonization by fungi of the genus of Alternaria and the content of the two alternaria toxins: alternariol (AOH) and alternariol monomethyl ether (AME). The most frequently occurring species was A. alternata. The level of colonization of the seeds by Alternaria fungi depended mainly on the weather conditions prevailing during the growing season. Higher content of AOH and AME was observed in the seeds which were colonised the most by the fungus of Alternaria.
11

Wpływ bakterii fermentacji propionowej i mlekowej na wytwarzanie toksyn fuzaryjnych w badaniach modelowych

81%
Gwiazdowski R., Gwiazdowska D., Wickiel G.
Progress in Plant Protection
|
2010
|
tom 50
|
nr 4
In the presented work the influence of mixture of Propionibacterium freudenreichii and Lactobacillus rhamnosus cultures and supernatants on growth and mycotoxins production by Fusarium culmorum were investigated. The effect of tested mixtures on the fungus growth was evaluated by the plating method. F. culmorum was more sensitive on cultures mixture than supernatants mixture. In the second part of this work the influence of cultures and supernatant mixtures on production of the most important mycotoxins by F. culmorum in grain was estimated. It was found that the concentration of zearalenone was significantly lower in the presence of culture mixtures and supernatant mixture applied in the amount 2 ml into sample. The concentration of nivalenol was on the same level in the majority of trials. Only in the sample with 2 ml of supernatant mixture the amount of nivalenol was significantly lower in comparison with the control. Unexpectedly the deoxynivalenol concentration was significantly higher in almost all samples with cultures and supernatants mixtures.
12

The risk of winter oilseed rape contamination by ochratoxin A, depending on their storage conditions

81%
Wickiel G., Gwiazdowski R., Marcinkowska K.
Progress in Plant Protection
|
2012
|
tom 52
|
nr 3
Storage conditions are an important factor in colonization of agricultural goods by pathogenic fungi and contamination by mycotoxins. The aim of this study was to determine the effect of the winter oilseed rape storage conditions on the grain contamination by ochratoxin A. The study was conducted at the Department of Plant Protection Research Institute of Plant Protection – National Research Institute in Poznan. Mycological analysis of the examined seeds of the oilseed rape showed the presence of fungi such as Penicillium spp., and Aspergillus spp. Chromatographic analysis showed very low levels of ochratoxin A in the rapeseed tested immediately after harvest, as well as in the samples of seeds stored in the conditions simulating storage (below the quantitation limit) and low levels of ochratoxin A in the samples stored in the conditions that stimulate the secretion of toxins. The highest contamination with toxins was found in a sample of seeds of the Monolith and it amounted to 0.2 μg/kg OTA (ochratoxin A).
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