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Molecular identification of Cryptosporidium parvum in rabbits (Oryctolagus cuniculus) in Nigeria

100%
Ayinmode A. B., Agbajelola V. I.
Annals of Parasitology
|
2019
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tom 65
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nr 3
Rabbits are commonly reared by households and farmers in Nigeria as a source of meat, but there is no information available on Cryptosporidium genotypes occurring in rabbits in Nigeria. Fecal samples were collected from 107 rabbits and examined by modified Ziehl-Neelsen technique for the presence of Cryptosporidium oocysts. An infection rate of 3.7% (4/107) was obtained and all microscopy-positive samples were genotyped and subtyped to determine the circulating Cryptosporidium species using sequence analysis of the 18S rRNA gene and 60-kDa glycoprotein (gp60) gene, respectively. All the four microscopy-positive samples were identified as C. parvum by 18S rRNA gene. However, analysis of the gp60 gene revealed the presence of C. parvum subtype IIc, which is commonly found in humans in two isolates. These findings indicate natural infection of rabbits with C. parvum and underscore the need to investigate the probable role of animal hosts in the epidemiology of Cryptosporidium infection. This is the first report on genetic characterization of Cryptosporidium infecting rabbits in Nigeria.
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Cryptosporidiosis in a fire skink (Lepidothyris fernandi) and molecular identification of infecting species

100%
Ayinmode A. B., Agbajelola V. I.
Annals of Parasitology
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2018
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tom 64
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nr 1
Cryptosporidiosis is an infectious protozoan disease that affects a wide range of animals including reptiles. This is the first report of cryptosporidiosis in a fire skink (Lepidothyris fernandi), an insectivorous reptile commonly found in tropical West Africa. Faecal sample was collected from a fire skink at necropsy for the detection of parasites by faecal sedimentation method, Ziehl-Neelsen (ZN) acid-fast staining, Nested Polymerase Chain Reaction (PCR) and Nucleotide sequencing. Sections of the intestines were also processed for histopathology. Light microscopy revealed the presence of Ophidascarids sp. eggs and Cryptosporidium oocysts. Amplification of the 18S rRNA gene and nucleotide sequencing confirmed Cryptosporidium varanii as the infecting species. Histopathology revealed cellular infiltration and disruption of the epithelial cells along the brush border characteristic of intestinal inflammation.
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Detection and molecular identification of Cryptosporidium species in laboratory rats (Rattus norvegicus) in Ibadan, Nigeria

80%
Ayinmode A. B., Ogbonna N. F., Widmer G.
Annals of Parasitology
|
2017
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tom 63
|
nr 2
To study the occurrence of Cryptosporidium infection in laboratory rats (Rattus norvegicus) raised for experimental usage, 134 faecal samples were obtained from two rearing houses in Ibadan and examined for the presence of Cryptosporidium oocyst using the modified acid fast staining technique. Cryptosporidium species in 2 samples positive for microscopy were further characterized by a nested polymerase chain reaction (PCR) amplifying the 18S rRNA gene. Two of 134 samples were positive for the Cryptosporidium oocysts. Sequencing of the small-subunit rRNA amplicons identified the species in the two PCR positive samples as Cryptosporidium andersoni and Cryptosporidium rat genotype. These findings showed that laboratory rat is a potential reservoir for diverse Cryptosporidium species and suggests that laboratory rats should be screened for Cryptosporidium infection prior to experiments, especially where pathogen free animals are not available. This the first report to identify Cryptosporidium species infecting laboratory rats in Nigeria.
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