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Lead (Pb) contamination of urban soil is a threat to human health, and reducing the risk of Pb-contaminated soil is a continuing international concern. Soil stabilization technique has been considered as apromising remediation technique to facilitate the immobilization of Pb in soil. The purpose of this study was to evaluate the effects of different amendments, including H₃PO₄, Ca(H₂PO₄)₂, H₃PO₄+Ca(H₂PO₄)₂, and zeolite on Pb levels in two naturally contaminated urban soils. Pb speciation and bioavailability was evaluated by the sequential extraction test (SET) and the toxicity characteristic leaching procedure (TCLP) after a two-month incubation. The application of phosphorus amendments significantly reduced the TCLP-Pb concentration in two soil types and the SET-Pb in Soil 1 (alkaline but with high Pb level), whereas the zeolite amendment diminished the SET-Pb in two soil types and the TCLP-Pb in Soil 1. Nevertheless, regardless of the soil type, the application of phosphorus amendments resulted in a significant increase of residual Pb. These phosphate amendments may be a viable strategy in the in situ remediation of Pb contamination in urban soils.
Mitogen-activated protein kinases (MAPKs) play important roles in signal transduction pathways responding to various stresses. In this study, we described the molecular characteristics of six MAPK genes (MaMAPK1, MaMAPK2, MaMAPK3, MaMAPK4, MaMAPK5 and MaMAPK6) cloned from banana (Musa acuminate L. AAA group, cv. Cavendish) using a RACE-PCR-based strategy. The predicted molecular masses of these MAPKs ranged from 43.0 to 70.1 kDa and their pIs ranged from 5.67 to 9.32. At the amino acid level, they shared high sequence similarity with MAPKs in the banana DH-Pahang (AA group) genome. Phylogenetic analysis showed that the deduced amino acid sequences of MaMAPKs also had high similarity with MAPKs of other plant species. These genes were expressed in nearly all tissues using semi-quantitative RT-PCR. All of the six MaMAPKs were induced by signal molecules in banana seedlings, such as ABA, Ethephon, MeJA and SA. The gene expressions of MaMAPK1, MaMAPK2, MaMAPK3, MaMAPK5 and MaMAPK6 were induced in resistant cultivar of banana after being inoculated with Fusarium Oxysporum f specialis (f. Sp) cubense Tropical Race 4 (Foc TR4). Our results suggest that MaMAPKs play a key role in both the development of banana seedlings and banana resistance to Foc TR4.
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