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1

Zmiennosc somaklonalna w kulturach in vitro

100%
Plader W.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
1997
|
tom 50
65-70
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Obtaining of hybrids within the family Cucurbitaceae by in vitro culture of immature embryos. III. Characteristics of hybrids of Cucurbita maxima x C.ficifolia and C.maxima x C.foetidissima

63%
Plader W., Rakoczy-Trojanowska M.
Genetica Polonica
|
1994
|
tom 35
|
nr 1-2
11-22
3

Redagowanie RNA w chloroplastach. Co wiadomo na temat regulacji tego procesu?

63%
Guzowska-Nowowiejska M., Plader W.
Postępy Biologii Komórki
|
2010
|
tom 37
|
nr 2
343-361
4

Analiza aktywnosci transkrypcyjnej genomu przy zastosowaniu mikromacierzy dachowkowatych

51%
Zmienko A., Guzowska-Nowowiejska M., Plader W., Figlerowicz M.
Biotechnologia
|
2008
|
nr 4
101-114
5

The sequencing and analysis of cucumber chloroplast genome

51%
Plader W., Yukawa Y., Sugiura M., Malepszy S.
Acta Physiologiae Plantarum
|
2004
|
tom 26
|
nr 3 suppl.
6

The complete structure of the cucumber [Cucumis sativus L.] chloroplast genome: its composition and comparative analysis

51%
Plader W., Yukawa Y., Sugiura M., Malepszy S.
Cellular and Molecular Biology Letters
|
2007
|
tom 12
|
nr 4
584-594
The complete nucleotide sequence of the cucumber (C. sativus L. var. Borszczagowski) chloroplast genome has been determined. The genome is composed of 155,293 bp containing a pair of inverted repeats of 25,191 bp, which are separated by two single-copy regions, a small 18,222-bp one and a large 86,688-bp one. The chloroplast genome of cucumber contains 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes (4 rRNA species), and 37 tRNA genes (30 tRNA species), with 18 of them located in the inverted repeat region. Of these genes, 16 contain one intron, and two genes and one ycf contain 2 introns. Twenty-one small inversions that form stem-loop structures, ranging from 18 to 49 bp, have been identified. Eight of them show similarity to those of other species, while eight seem to be cucumber specific. Detailed comparisons of ycf2 and ycf15, and the overall structure to other chloroplast genomes were performed.
7

Chromosome identification using fluorescent dyes for further physical mapping in cucumber [Cucumis sativus L.]

51%
Hoshi Y., Plader W., Malepszy S.
Journal of Applied Genetics
|
1996
|
tom 37A
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Sequential fluorescent staining with CMA and DAPI for somatic chromosome identification in cucumber [Cucumis sativus L.]

51%
Plader W., Hoshi Y., Malepszy S.
Journal of Applied Genetics
|
1998
|
tom 39
|
nr 3
Each of the seven chromosomes in cucumber (Cucumis sativus L.) was identified using sequential staining with Chromomycin A₃ (CMA) and 4-6-diamidino-2-phenylindole (DAPI) as DNA base-specific fluorescent dyes. The present method using enzymatic digestion produced a high level of well-spread early-metaphase chromosome complements. After CMA and DAPI staining, reproducible fluorescence bands were obtained in mitotic prometaphase chromosomes. The CMA staining method made it possible to characterize whole chromosomes from prometaphase to mid-metaphase. Chromosome 1 had the largest and widest CMA-positive (CMA⁺) band from the proximal region to the interstitial region on the long arm in prometaphase. A large gap separating of the short arm from the long arm was always observed in chromosome 2 during prometaphase. The banding pattern of the short arm was similar to that of the long arm in chromosome 2. Chromosomes 1 and 2 in early metaphase had sharp and large CMA-positive and DAPI-negative (CMA⁺DAPI⁻) bands at the pericentromeric regions. In early metaphase, chromosome 3 was characterized by having a narrow CMA⁺DAPI⁻ band on the pericentromeric region of the short arm. Chromosomes 4 and 5 showed similar chromosome length and had a large CMA⁺ band at the distal region of the long arm. Chromosome 4 did not show any clear band in the short arm, while chromosome 5 showed a telomeric CMA⁺ band at the short arm and a clear CMA⁺DAPI⁻ band at the pericentromeric region. Chromosome 6 had a CMA⁺ band at the distal region and a weak CMA⁺ band at the proximal region in each of the arms. Chromosome 7 had an evident CMA⁺ band in the long arm and a CMA⁺ DAPI⁻ band in the pericentromeric region.
9

Transgene inheritance in plants

51%
Yin Z., Plader W., Malepszy S.
Journal of Applied Genetics
|
2004
|
tom 45
|
nr 2
10

Transgenic cucumber - a current state

45%
Yin Z., Plader W., Wisniewska A., Szwacka M., Malepszy S.
Folia Horticulturae
|
2005
|
tom 17
|
nr 1
73-90
11

Chloroplast transformation reveals that tonbacco ycf5 is involved in photosynthesis

45%
Tsuruya K., Suzuki M., Plader W., Sugita C., Sugita M.
Acta Physiologiae Plantarum
|
2006
|
tom 28
|
nr 4
Hypothetical chloroplast reading frame 5 (ycf5) is encoded by the chloroplast genome and potentially specifies a protein of 313 amino acids in tobacco. A northern blot analysis showed that ycf5 transcripts accumulated at substantial levels in the chloroplasts, but not in the proplastids of nonphotosynthetic cultured cells. In an attempt to determine the function of ycf5, we constructed mutant alleles for the targeted deletion of ycf5. The mutant allele was introduced into the tobacco chloroplast genome by biolistic chloroplast transformation to replace the corresponding wild-typeycf5 alleles by homologous recombination. Homoplasmic ycf5-knockout tobacco plants displayed a pale-green phenotype. An analysis of the transient increase in chlorophyll fluorescence suggested that the electron flow around photosystem II (PSII) was completely impaired in the ycf5-deficient transformants. These findings indicate that ycf5 is indeed a functional gene, and that its gene product is involved in the generation of functional PSII units. This is the first report of the isolation and characterization of ycf5-knockout mutants in higher plants.
12

Biological significance, computational analysis, and applications of plant microRNAs

39%
Szwacka M., Pawelkowicz M., Skarzynska A., Osipowski P., Wojcieszek M., Przybecki Z., Plader W.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 08
13

Gen CsWIP1 o domenie palca cynkowego Cys2/His2 w zmutowanej linii ogórka 2gg o recesywnym locus gygy

39%
Siedlecka E., Skarzynska A., Majewska A. G., Wojcieszek M., Pawelkowicz M., Plader W., Przybecki Z.
Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin
|
2019
|
nr 285
14

The DefH9-iaaM-containing construct efficiently induces parthenocarpy in cucumber

39%
Yin Z., Malinowski R., Ziolkowska A., Sommer H., Plader W., Malepszy S.
Cellular and Molecular Biology Letters
|
2006
|
tom 11
|
nr 2
Parthenocarpy (seedless fruits) is a desirable trait that has been achieved in many plant cultivars. We generated parthenocarpic cucumber fruits by introducing the chimeric DefH9-iaaM construct into the cucumber genome using an Agrobacterium tumefaciens-mediated protocol. The construct consists of the DefH9 promoter from Antirrhinum majus and the iaaM coding sequence from Pseudomonas syringae. Transgenic plants were obtained from nine independent transformation events: half of these were tetraploid and did not produce seeds following self-pollination, while the remaining half were capable of displaying parthenocarpy in the subsequent reproductive generation. Of the fruits produced by the transgenic lines, 70–90% were parthenocarpic. The segregation of the marker gene in the transgenic T1 progeny indicated single gene inheritance. The seed set in the transgenic lines and their F1 hybrids was lower than in the non-transgenic control plants. Some of the methodological details and the practical significance of the results are discussed.
15

Chromosome number variation in somatic hybrids between transgenic tomato [Lycopersicon esculentum] and Solanum lycopersicoides

39%
Kulawiec M., Tagashira N., Plader W., Bartoszewski G., Kuc D., Sniezko R., Malepszy S.
Journal of Applied Genetics
|
2003
|
tom 44
|
nr 4
16

The metabolic profiles of transgenic cucumber lines vary with different chromosomal locations of the transgene

27%
Tagashira N., Plader W., Filipecki M., Yin Z., Wisniewska A., Gaj P., Szwacka M., Fiehn O., Hoshi Y., Kondo K., Malepszy S.
Cellular and Molecular Biology Letters
|
2005
|
tom 10
|
nr 4
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