Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 3

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Immunomodulatory capacity of Wharton’s Jelly mesenchymal stem cells after stimulation in vitro: cytokines and growth factors transcriptional response analysis

100%
Lech W., Zychowicz M., Figiel-Dabrowska A., Domanska-Janik K., Buzanska L.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: Mesenchymal Stem Cells (MSC) possess ability to release cytokines and growth factors that suppress immune responses and stimulate tissue regeneration. Wharton’s Jelly-derived MSC (WJ-MSC) in the addition to the strong adjuvant properties are characterized by low immunogenicity. AIM(S): The aim of this study is to verify immunomodulatory properties of WJ‑MSC after TNFα and IFNγ stimulation in vitro, by comparative analysis of the expression of cytokines and growth factors they produce. METHOD(S): WJ-MSC isolated from human umbilical cords were cultured in closed system that provides a constant 5% oxygen concentration. We compared immunomodulatory properties of WJ-MSC in 2D or 3D structures (scaffolds) made in our laboratory. Both of those cell populations were cultured in medium with/ without stimulate factors: TNF‑α and IFN‑γ. After stimulation, 2D and 3D cell cultures were characterized with quantitative RT-PCR for the expression of various cytokines and growth factors with non-stimulated 2D cells as an internal control. WJ-MSC grown in 3D were also characterized by live/dead cells presence which were labeled with calcein AM/ethidium homodimer. RESULTS: The obtained results indicated increased expression of mRNA in 3 D structures vs. control 2 D cells for almost all analyzed cytokines: IL‑6, TGF‑β1 , BDNF, GDNF, EGF, bFGF. Moreover, TNF‑α and IFN‑γ stimulation causes even further increase of mRNA expression of those cytokines in 3 D cultures compared to non-stimulated 2 D control. In our scaffolds models, the intercellular connections which were labeled in live cells with calcein AM have been observed already after 24h of culture and are visible also during the next days of analysis. CONCLUSIONS: Finally we can conclude, that WJ-MSC produce immunomodulatory factors, and their expression can be modulated by stimulation with chosen cytokines and 3D microenvironment. Such properties of WJ-MSC are important for the potential therapeutic application in the treatment of the diseases of inflammatory and autoimmune origin. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No STRATEGMED1/234261/2/NCBR/2014.
2

Lowered oxygen concentration and culture in 3-dimension structure enhance immunological response of WJ-MSC to proinflammatory factors

100%
Figiel-Dabrowska A., Lech W., Zychowicz M., Domanska-Janik K., Sarnowska A.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
INTRODUCTION: MSC-based therapy is becoming more and more common treatment of various diseases, albeit still as an experimental approach. According to present‑day literature, the therapeutic effects of transplanted cells would not be ascribed to their differentiation, trans‑differentiation or repopulation but rather to their paracrine effect on damaged tissue. This way of treatment can be initiated and enhanced by local environmental mediators. AIM(S): The aim of this study was to assess the influence of inflammation specific environment in vitro on secretory WJ-MSC properties and to evaluate the possibility of programmed and controlled induction and enhancement of anti‑inflammatory cell properties in the context of further cell therapy. METHOD(S): Our experiments were based on reconstruction in vitro the environment to which therapeutic cells (WJ‑MSC) are usually transplanted. The inflammatory conditions that occur around the transplant were reproduced through TNFα and IFNγ stimulation. Tissue specific oxygen concentration (5%), 3‑dimension transplant structure and chemical composition of the indirect transplanted cell surrounding as determined by additional scaffold ingredients (fibrin and platelet lysate) were also reconstituted. RESULTS: Carried experiments have shown specific changes in the secreted cytokine pallet induced in vitro by the inflammation‑like WJ‑MSC surrounding. We have proved that environmental modifications cause changes in synthesis and secretion of the determined proteins. Both, the physioxia introduced in our in vitro experiments and WJ cells cultured in 3-dimensional structures enhanced cytoprotective paracrine properties of WJ-MSC. Additional reinforcing effect was observed when therapeutic cells were transplanted on platelet lysate – containing scaffolds. CONCLUSIONS: Presented results indicate that by optimization of cell culture and transplantation conditions we could control and enhance cytokine-connected therapeutic properties of MSC. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No Strategmed 1/234261/2/NCBR/2014.
3

Preclinical characteristics and regenerative potential of adipose – derived MSC

58%
Sarnowska A., Figiel-Dabrowska A., Szczepanik E., Kuzma M., Mierzewska H., Antczak-Marach D., Kruk M., Terczynska I., Krzesniak N., Sawicka E., Nowak A., Noszczyk B., Kaminska A., Marchel A., Domanska-Janik K.
Acta Neurobiologiae Experimentalis
|
2017
|
tom 77
|
nr Suppl.1
While the rapid development of stem cell-based therapies are nowadays running, the reliable protocols leading to safe and effective way for cell isolation, expansion and commitment in vitro according to distinguished therapeutic purposes still need more consensus and standardization. In order to obtain particular disease-committed therapeutic cells we have screened various culture conditions, especially new systems involving lowered oxygen tension and small molecule treatments which may “rejuvenates” MSC. We also looked on the changes in grow dynamics of long-term cultures in various oxygen concentrations to dissect changes that predict genetic instability of cultivated cells. The standardization of such type of culture (by additional criteria beyond the framework developed by ISCT) should further enhance life-span, expansion and differentiation potential of MSCs needed for more effective regeneration of diseased brain. In recent years we have also entered into the clinic with individual medical experiments (in accordance with the guidelines described by A. Korczyn’ Sieratzki – Chair of Neurology Tel Aviv University 2010) based on mesenchymal regenerative cell therapy to elaborate save therapeutic procedures for autoimmune epilepsy, ALS and vast nerve injury. First results are promising and indicate regenerative and immunomodulatory properties of transplanted MSC. FINANCIAL SUPPORT: The work was supported by National Centre for Research and Development grant No STRATEGMED 1/234261/2/NCBR/2014.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.