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1

The oxydation of malate by mitochondria isolated from germ roots of lupin

100%
Tomaszewska B., Krzyminska S.
Applied Biology Communications. Lublin Scientific Center
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1994
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tom 04
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nr 5-6
2

Cytotoxic activity of Serratia marcescens clinical isolates

81%
Krzyminska S., Raczkowska M., Kaznowski A.
Polish Journal of Microbiology
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2010
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tom 59
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nr 3
Twenty Serratia marcescens isolates from clinical specimens were examined for their cytotoxic activity on four cell lines (HEp-2, Vero, CHO, J774). Most of the isolates were found to be cytotoxic to CHO (70%), Vero (75%) and HEp-2 cells (90%). CHO cells were the most sensitive to cell-free supematants, followed by HEp-2 and Vero cells. Two strains produced cytotonic toxins which caused elongation of CHO cells. Moreover, twelve isolates (60%) revealed cytotoxic potential to macrophage cell line J774. The results indicate that these bacteria may destroy phagocytes and epithelial cells, which may lead to spread within the host.
3

Evaluation of the pathogenic potential of insecticidal Serratia marcescens strains to humans

81%
Konecka E., Mokracka J., Krzyminska S., Kaznowski A.
Polish Journal of Microbiology
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2019
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tom 68
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nr 2
We observed the death of insect caterpillars of Spodoptera exigua in the laboratory culture line and identified Serratia marcescens as the bacterial causative agent of the insect death. We confirmed that S. marcescens had insecticidal activity against S. exigua and caused high mortality of larvae. The LC₅₀ values of S. marcescens CFU per 1cm² of insect diet surface were similar for all isolates. Our research reports novel strains with high pesticidal activity as candidates for future research on a new bioinsecticide. As bioinsecticides cannot be harmful to non-target organisms, we determined the pathogenic properties of S. marcescens to humans. We proved the ability of S. marcescens todamage mammalian epithelial cells. All strains had cytopathic effects to Vero cells with a cytotoxic index ranging from 51.2% ± 3.8%to 79.2% ± 4.1%. We found that all of the strains excreted catecholate siderophore – enterobactin. All isolates were resistant to sulfameth-oxazole, tobramycin, gentamicin, cefepime, and aztreonam. We did not observe the ESBL phenotype and the integrons’ integrase genes. Resistance to sulfamethoxazole was due to the presence of the sul1 or sul2 gene. The use of resistant S. marcescens strains that are pathogenic to humans in plant protection may cause infections difficult to cure and lead to the spread of resistance genes. The results of our study empha-size the necessity of determination of the safety to vertebrates of the bacteria that are proposed to serve as biocontrol agents. The novelty of our study lies in the demonstration of the indispensability of the bacteria verification towards the lack of hazardous properties to humans.
4

Enteropathogenic activity and invasion of HEp-2 cells by Aeromonas caviae clinical isolates

81%
Krzyminska S., Kaznowski A., Lindner K., Mnichowska M.
Acta Microbiologica Polonica
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2003
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tom 52
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nr 3
5

Purification and characterization of cytolytic toxins produced by Aeromonas hydrophila and Aeromonas veronii biotype sobria strains

81%
Krzyminska S., Kaznowski A., Spychala H.
Polish Journal of Microbiology
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2006
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tom 55
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nr 1
Cytolytic toxins produced by Aeromonas hydrophila and Aeromonas veronii biotype sobria strains were partially purified from culture filtrates by two steps of purification: ammonium sulfate precipitation and hydrophobic chromatography using Phenyl-Sepharose CL-4B. Hemolytic activity was detected in one or two peaks in elution profile. Purified toxins were also cytotoxic to Vero and CHO cells. Moreover, these toxins revealed cytotonic activity to CHO cells.
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