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Identification and characterization of a small heat shock protein 17.9-CII gene from faba bean (Vicia faba L.)

100%

Kumar R., Lavania D., Singh A. K., Negi M., Siddiqui M. H., Al-Whaibi M. H., Grover A.

Acta Physiologiae Plantarum

2015

tom 37

nr 09

We cloned and characterized the full-length coding sequence of a small heat shock protein 17.9 gene from faba bean encoding 160 amino acids and containing the conserved a-crystallin domain at the C-terminus. Homology and phylogenetic analysis suggested its proximity with the class II sHsp members of fabaceae family. Therefore, we name this gene as VfHsp17.9-CII. The VfHsp17.9-CII transcript showed a clear heat stress induction pattern in leaves of young seedlings and flowering plants. Transient expression of VfHsp17.9-CII fused with green fluorescent protein reporter indicated its nuclear localization. Overexpression of recombinant VfHsp17.9- CII protein in Escherichia coli cells increased tolerance of the bacterial cells to heat and arsenic stresses. The reduction of faba bean pollen viability in response to heat stress correlated with the accumulation pattern of VfHsp17.9-CII transcript in heat stressed pollen. It is suggested that VfHsp17.9-CII protein plays a key role in heat and heavy metal stress tolerance.

Water quality of the River Ganga [Garhwal Himalayas]

84%

Singh H. R., Nautiyal P., Dobriyal A. K., Pokhriyal R. C., Negi M., Baduni V., Nautiyal R., Agarwal N. K., Nautiyal P., Gautam A.

Acta Hydrobiologica

1994

tom 36

nr 1

3-15

The water quality of the River Ganga was monitored using certain physical, chemical, and biological characteristics. As compared with abiotic, the biological quality was found to vary considerably. The quanta and spectra of plankton and macrozoobenthos increased with the river`sdescent. The quanta - spectra relationship for the selected components (r=0,72,0,69)was found to be meaningful. The study indicates an oligotrophic state of the River Ganga

Ograniczanie wyników

1 Acta Hydrobiologica

1 Acta Physiologiae Plantarum

2 Negi M.

1 Agarwal N. K.

1 Al-Whaibi M. H.

1 Baduni V.

1 Dobriyal A. K.

1 Gautam A.

1 Grover A.

1 Kumar R.

1 Lavania D.

1 Nautiyal P.

1 Nautiyal R.

1 Pokhriyal R. C.

1 Siddiqui M. H.

1 Singh A. K.

1 Singh H. R.

1 2015

1 1994

Wyniki wyszukiwania

Identification and characterization of a small heat shock protein 17.9-CII gene from faba bean (Vicia faba L.)

Water quality of the River Ganga [Garhwal Himalayas]