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Bilge keels are effective passive devices in mitigating the rolling motion, and the usage of them covers almost all the sea going vessels. This paper focuses on the viscous effect of the bilge keel, ignored the effect of the free surface and the effect of the ship hull, for the general viscous characteristic of the bilge keel. In order to investigate the viscous effect of the bilge keel on the total damping moment, a special 2 dimensional numerical model, which includes a submerged cylinder with and without bilge keels, is designed for the simulation of forced rolling. Three important factors such as bilge keels width, rolling periods, as well as maximal rolling angles are taken into account, and the viscous flow field around the cylinder is simulated by some codes based on the viscous method in different conditions, in which the three factors are coupled. Verification and validation based on the ITTC method are performed for the cylinder without bilge keels in the conditions of different rolling periods and maximal rolling angles. The primary calculation of damping moment induced by the cylinder with 0mm, 4mm, and 10mm width bilge keels shows some interesting results, and a systematic analysis is conducted. The analysis of the damping moment components suggests there is phase difference between the damping moment induced by the cylinder and the bilge keels, and when the bilge keels width reaches a special size, the total damping moment is mitigated. The calculation of the damping moments induced by the cylinder with some larger bilge keels are also performed, and the results suggest that, the damping moment induced by the bilge keels is increased rapidly and becomes the dominant part in the total damping moment while the width of the bilge keels are increased, but the damping moment induced by the cylinder is not changed significantly. Some illustration of the vortices formation and shedding is included, which is the mechanism of the damping moment caused by the bilge keels. The present work shows an interesting problem, and it is useful for the bilge keel design
Chilling stress has a strong negative impact on the growth and development of winter wheat (Triticum aestivum L.). To investigate the recovery of physiological function and yield formation by plant growth regulators following chilling stress, we performed low-temperature phytotron experiments at the booting stage, and sprayed 6-benzylamino adenine (6-BA), salicylic acid (SA), brassinolide (BR) and abscisic acid (ABA) after chilling stress. Plant growth regulators significantly enhanced SPAD value and net photosynthetic rate (Pn) in flag leaves following chilling stress (p < 0.05). Compared with the control group sprayed with distilled water, stomatal conductance (Gs) and transpiration rate (Tr) increased, while intercellular carbon dioxide concentration (Ci) decreased. In addition, the concentration of malondialdehyde (MDA) was significantly decreased, and the activities of superoxide dismutase (SOD) and peroxidase (POD) were enhanced (p < 0.05). Plant growth regulators also increased the grain filling rate during the 14 days after anthesis, thereby increasing grain weight. The grain number per spike, 1000-kernel weight, and yield per plant after harvest were also significantly enhanced (p < 0.05). Thus, spraying plant growth regulators at the booting stage relieved the adverse effects on physiological activity in wheat flag leaves caused by chilling stress, and 6-BA and SA were particularly effective.
DNA methylation plays an important role in gene expression regulation during biological development in plants. To explore the mechanism of chemically induced male sterility (CIMS) in wheat, using cDNA-amplified fragment length polymorphism (cDNA-AFLP) and methylation-sensitive amplification polymorphism (MSAP) approaches, 6.66 and 3.42 % of the sequences showed changes in gene expression and DNA methylation in 1376-CIMS as compared to its fertility line 1376. We sequenced 54 fragments that differed in cDNA-AFLP and 26 DNA fragments which differentially displayed in MSAP comparisons of CIMS and 1376. Our results provided evidences for genome-wide changes in gene expression and DNA methylation occurring in the development of the 1376-CIMS system induced by chemical hybridizing agent SQ-1, and its counterpart fertility line 1376. Moreover, this study contributed to the elucidation of CIMS effects on responses of transcriptome and methylome in the development of the anther.
Protein polyubiquitination is a significant regulator of diverse physiological functions, including sexual reproduction, in plants. Chemical hybridizing agents (CHA) SQ-1 has been shown to induce male sterility in wheat (Triticum aestivum L.) through inhibition of pollen development. This mechanism by which CHA induces male sterility in wheat is unclear. In this study, differential proteomic analysis of polyubiquitinated proteins associated with wheat male sterility was investigated. Wheat plants of the same genetic background were treated with or without CHA. Ubiquitinated proteins were then extracted and enriched for proteomic analysis. Differentially expressed polyubiquitinated proteins in trinuclear stage anther were identified by nanospray liquid chromatography/tandem mass spectrometry. A total of 127 and 131 differentially expressed polyubiquitinated proteins, including heat shock protein 70, ATPase subunit, glycosyltransferase, ubiquitinrelated enzyme, and 20S proteasome subunit, were successfully identified by searching against wheat protein database and NCBInr database, respectively. Most of these proteins are related to photosynthesis, carbohydrate and energy metabolism, and multiple metabolic processes. These findings show that alteration of polyubiquitinated proteins is associated with male sterility in wheat.
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