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During 1985—1990 12 302 fodder samples were tested bacteriologically, including 303 g (24,7%) from fodder mixtures, 1585 (12,89%) from fish meal, 2905 (23,61%) from bone-meateal, 4054 (32,95%) from imported ground soya beans and peanuts 719 (5,85%) samples of protein- fat concentrate. Out of 12 302 tested samples 456 (3,71%) Salmonellae were isolated. Salmonella was most often isolated from protein-fat concentrate (14.88%) and bone-meat meal (8,57%). This bacterium was isolated with the lowest frequency from imported ground grain (0,69%) and fish meal (0,76%). The following serotypes were most commonly isolated: Salmonella choleraesuis, S. derby, S. isangi and S. typhimurium.
The examinations were performed in 1988—1990. The purpose of the work was to investigate the survival of Salmonella in the soil and water. Salmonellae originated from dead or killed foxes and swabs taken from the vagina or anus. The isolated strains were classified as S. choleraesuis, S. dublin, S. enteritidis and S. typhimurium. Seedings were done on solid media, i.e. basic agar medium, the BGA, Endo, McConkey’s and Slavin’s media and liquid media, i.e. MK and SF. An average period of survival in the soil of individual serotypes was: S. choleraesuis — 257 days, S. dublin — 381 days, S. enteritidis — 451 days and S. typhimurium — 321 days. An average survival period in water was: S. choleraesuis — 18 days, S. dublin — 14 days, S. enteritidis — 25 days and S. typhimurium — 22 days.
Przeprowadzone badania wykazały, że w określeniu toksynotwórczości badanych serotypów pałeczek Salmonella większą przydatność wykazał test na oseskach mysich (10°/o czulszy) w porównaniu z testem na podwiązanych pętlach jelita kró­lika. Najwyższą toksynotwórczość wykazał serotyp S. dublin, natomiast brak tok­synotwórczości stwierdzono u serotypu S. choleraesuis. W określeniu zjadliwości indeks fagocytarny okazał się mniej przydatny od próby biologicznej na białych myszkach. Najbardziej zjadliwy w obu testach był serotyp S. typhimurium, naj­mniej zaś serotyp S. choleraesuis.
The aim of this experiment was to breed a cardio-pulmonar syndrome in common foxes with the help of a taurine deficit and to examine the influence of this amino acid on the prevention and treatment of the disease. The experiment was conducted on two fox farms in the period from July to December. On the first farm, 90 (5-6 week-old) animals were randomly chosen from among 1100 pups. They were divided into three equal groups. The first control group (C-group) consisted of animals which were fed in the same way as other animals. Meat components constituted 65-70% of the feed; vegetable components with vitamin-mineral supplements constituted 30-35% of the feed. The foxes in the second group (E - experimental) and third group (ET - experimental with taurine) were fed with the same feed as those from the first group, but the proportions of meat and vegetable components were inverted and all feed components were cooked. Taurine was added to the feed in the ET group. The average taurine contents in blood serum and bile was the lowest in the E group, and in spite of its low level foxes did not suffer from lung-heart syndrome. On the second farm, where cases of lung-heart syndrome occured, 150 pups were given normal feed with taurine suplement, and 150 pups - feed with no taurine suplement. The disease occured in 7 (4.66%) and 9 (6.0%) foxes, respectively. Two foxes in each group were cured - that is 28.57% and 22.2%, respectively.
The aim of the study was to determine the etiological factors of an outbreak of cattle illness with symptoms nervous disorders. The disease appeared simultaneously in adult animals on three dairy farms between August and October. The majority of sick animals was after parturition and had high milk production. The diagnosis gene was found through clinical anatomopathology, histopathology and bacteriological examinations of the animals, as well as molecular, serological, virusological and chemical tests. L. monocytogenes was isolated from the brain tissue of the dead animals. Restriction analysis (PCR-RFLP) of the DNA fragment of the hly A gene from L. monocytogenes facilitated differentiation between the virulent and control strains. It was found that the copper and selenium levels in the cows' serum were reduced significantly. Additional examinations indicated the low content of these minerals in the forage.
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