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1

The effect of low temperature and GA3 treatments on dormancy breaking and activity of antioxidant enzymes in Fritillaria meleagris bulblets cultured in vitro

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Petric M., Jevremovic S., Trifunovic M., Tadic V., Milosevic S., Dragicevic M., Subotic A.
Acta Physiologiae Plantarum
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2013
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tom 35
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nr 11
We investigated the effect of low temperature and gibberellic acid (GA3) treatment on dormancy in Fritillaria meleagris L. bulbs. Also, we studied the effect of dormancy breaking on the antioxidant enzymes activity. To overcome dormancy, bulbs require a period (4–8 weeks) of exposure to low temperature. Bulbs regenerated in vitro were grown in the dark on medium without growth regulators at the standard (24 °C) or at low temperatures (4 and 15 °C) for 4, 6, 8 and 10 weeks. Bulbs were collected after 3, 4 and 5 weeks of cooling at 4 °C. To investigate the influence of GA3 on dormancy, bulbs were treated for 24 h with GA3 solutions with 1, 2 and 3 mg l−1 concentrations. During the period of growth of bulbs at 4 °C, regeneration of bulbs was very weak, while at 15 °C the number of regenerated bulbs increased significantly. Improved bulb sprouting was achieved by a short treatment with gibberellin. Low temperature also represents a kind of oxidative stress for the plant. The activity of superoxide dismutase, catalase (CAT) and peroxidase (POX) in bulbs of F. meleagris L. grown in vitro and ex vitro increased with decreasing temperature in contrast to glutathione reductase. POX showed generally lower activity than CAT which indicates that major role in the breaking dormancy and preparing bulbs for sprouting have catalases.
2

Assessment of in vitro multiplication of Lemna minor in the presence of phenol: plant/bacteria system for potential bioremediation - part I

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Radulovic O., Petric M., Raspor M., Tadic V., Jovanovic P., Zecevic W.
Polish Journal of Environmental Studies
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2019
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tom 28
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nr 2
The aim of this work was to examine the multiplication of the common duckweed (Lemna minor), an aquatic plant species widespread in European stagnant waters, in two different media (Murashige – Skoog and Hoagland) with and without phenol supplementation. In order to quantify plant multiplication we have used relative growth rate and tolerance indices on both tested media and at five phenol concentrations (10, 15, 20, 30 and 100 mg/L). Furthermore, we examined the possibility of phenol removal from aqueous media containing different phenol concentrations, by using plant/bacteria system consisting of the duckweed and its naturally occurring microbial populations. After 7 days, number of newly formed fronds was approximately four times higher than at the beginning of the experiment on both tested media. The most important result in this study was removal of 70% of phenol from the highest initial concentration of 100 mg/L, in mixed cultures of duckweed and bacteria. By comparison, aseptic duckweed cultures removed approximately 50% of phenol at the same initial concentration. Our duckweed specimen showed a fast reproduction rate, high tolerance to phenol and a possible cooperation with rhizosphere-associated bacteria. All of these traits can be ultimately utilized for bioremediation purposes.
3

Culture-dependent analysis of 16S rRNA sequences associated with the rhizosphere of Lemna minor and assessment of bacterial phenol-resistance: plant/bacteria system for potential bioremediation - part II

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Radulovic O., Petric M., Raspor M., Stanojevic O., Janakiev T., Tadic V., Stankovic S.
Polish Journal of Environmental Studies
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2019
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tom 28
|
nr 2
In this work, we demonstrate that the rhizosphere of common duckweed (Lemna minor) is inhabited with various phenol-resistant bacterial strains. Based on 16S rRNA sequencing, we have identified 60 rhizosphere-associated bacterial isolates belonging to 10 different bacterial genera (Pseudomonas, Hafnia, Serratia, Enterobacter, Micrococcus, Stenotrophomonas, Xanthomonas, Bacillus, Staphylococcus and Klebsiella). All isolates have been tested for phenol resistance and ability to utilize phenol as the sole carbon source. 70% of all isolates survived high doses of phenol (≥200 mg/L) and at least 27% can be potentially acclimatized by gradual increase of phenol concentration. Finally, based on high phenol resistance, ability to utilize phenol as the sole carbon source and documented low pathogenicity, we propose 5 strains as potentially excellent candidates for bioremediation. These 5 strains taxonomically correspond to Klebsiella sp., Serratia sp., and Hafnia sp., respectively. To the best of our knowledge, this is the first attempt to assess decontamination capacity of Serratia nematodiphila and Hafnia sp. in the context of bioremediation of phenol-contaminated aqueous media. Although additional analyses are needed, interaction between the common duckweed and the selected bacterial strains may be utilized in future bioremediation strategies.
4

Quantification of arabinogalactan proteins during in vitro morphogenesis induced by beta-d-glucosyl Yariv reagent in Centaurium erythraea root culture

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Trifunovic M., Tadic V., Petric M., Jontulovic D., Jevremovic S., Subotic A.
Acta Physiologiae Plantarum
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2014
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tom 36
|
nr 05
Arabinogalactan proteins (AGPs) are a family of highly glycosylated cell surface proteins located at the plasma membrane and plant cell wall. AGPs play important roles in plant growth and development. Yariv phenylglycoside (βGlcY), synthetic red-brown dye that specifically binds and precipitates AGPs, has been used for detection and quantification of AGPs in plant tissue. Graded concentrations of βGlcY (0–75 μM) were used to investigate the effect of this synthetic dye on induction of in vitro morphogenesis in Centaurium erythraea root culture on two nutrient media: ½MS and ½MS + IBA 1.0 μM. Regeneration of C. erythraea shoots on root explants was stimulated on both media supplemented with 25 μM βGlcY after 8 weeks in culture. Quantification of AGPs in different tissues of C. erythraea was determinate with single radial diffusion method. This work emphasizes clear effect of βGlcY on induction of morphogenesis in vitro in C. erythraea root culture.
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