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The purpose of the investigations was to determine the acute toxicity of some plant protection products for earthworms. The methodical bases of the studies were OECD Guideline No 207 and Good Laboratory Practice (GLP). The test species was Eisenia fetida (Sav.). The tested preparations were introduced into artificial soil in concentrations of 62.5; 125, 250, 500 and 1000 mg·kg⁻¹ dry matter of soil. The test duration was 14 days. The animals were weighed following that time and alive animals were counted and observed. Taking into account the obtained results, the values of toxicity of pesticides: LC₀ (the highest concentration of the test substance causing no mortality of a population of earthworms under the conditions of the test), LC₅₀ (the concentration of the test substance that is expected to results in mortality of 50% of a population of earthworms under the conditions of the test) and LC₁₀₀ (the lowest concentration of the test substance causing 100% of mortality of a population of earthworms under the conditions of the test), were determined. It was found that influence of tested plant protection products on earthworms was diversified.
The response of soil microflora to increasing concentration of three pesticide preparations containing diazinon (insecticide), linuron (herbicide) and mancozeb + dimethomorph (fungicide) as an active substances, was determined under laboratory conditions. All pesticides were applied at three different dosages, i.e. 7, 35 and 700 mg kg⁻¹ soil for the insecticide, 4, 20 i 400 mg kg⁻¹ soil for the herbicide and 15, 75 and 1500 mg kg⁻¹ soil for the fungicide mixture. The lowest dosages corresponded to the maximum predicted environmental concentration (PEC) of pesticides applied in field conditions, while the other dosages were the multiplicities of PEC. After 1, 14 and 28 days of experiment the numbers of heterotrophic bacteria, fungi and bacteria involved in nitrogen transformation (nitrifying, denitrifying and N₂ -fixing bacteria) was estimated. The numbers of colony forming units (CFU) of respective groups of microorganisms were determined on selective media by means of the serial dilution technique and the spread plate method. The plate-count data indicated that pesticide used affected the numbers of tested microbial groups. All chemicals stimulated the numbers of heterotrophic bacteria and fungi at the higher dosages. However, the reverse effect was detected at the beginning of the experiment. In turn, bacteria involved in nitrogen turnover, particularly N₂ - fixing and nitrifying bacteria, were the most sensitive to the tested pesticides and their numbers were significantly reduced on each time-point. This great susceptibility of these physiological groups of microorganisms predispose them to be warning biomarkers for the side-effects of environmental pollution caused by chemicals.
The aim of this study was to evaluate the mineralization rates of chlormequat chloride (CCC) in soils of different textures from measurements of 14CO₂ evolution over a period of 100 days. The level of extractable and bound residual 14C in the soils at the end of the experiment was also determined. The degradation pattern of CCC was similar in all soil types and degree of its mineralization ranged from 53.4 to 55.7% over a period of 100 days. This process in all soils was described by first-order kinetics and CCC was mineralized with a similar constant rate (k) on the level of 0.029-0.034 day-1, and DT50 ranged from 67.7 days for sandy soil to 71.0 days for silt loam soil. Evaporation was not significant in the removal of CCC from soils, and the total amount of 14C-volatile organic compounds recovered at the end of the trials was similar to the background level. The level of 14C-extractable residues was low and reached the maximum value of 7.2% of the introduced radioactivity for sand and sandy loam soils. Levels of 14C-bound residues were higher in all soil types. The highest value (39%) was obtained for silt soil, while in the remaining soils it was lower and ranged from 30 to 33.5% of the introduced radioactivity. The relatively large amount of bound residues of CCC is probably connected with a strong affinity for soil components despite of its high water solubility and potential to be leached.
The degradation behaviour of 2,4-D and MCPA in four types of soil were determined from measurements of 14CO2 evolution over a period of 100 days. The total 14C-organic volatile compounds evaporated from the soils during the experimental period and the residual 14C in the soils at the end of the incubation period was also determined. The degree of mineralization was different for tested pesticides, and did not exceed 30% for 2,4-D or 46% for MCPA. The greatest mineralization of 2,4-D occurred in sandy soils containing the least amount of organic carbon, while in the case of MCPA, the highest level of mineralization was observed in loamy sand and silt loam soils. Volatilization was the most important mechanism of 2,4-D loss from soils and accounted for 46.6% of the total applied dose for sandy loam soil. The emission of volatile organic substances from MCPA-treated soils was lower, with the maximum value of 10.5% being emitted from silt soil. A significant amount of the introduced radioactive material was recovered as residues. The level of 14C-extractable residues for pesticides was low and ranged from 0.9% to 4.9% of total radioactivity. However, the level of 14Cbound residues was significantly greater and ranged from 14.6% to 43.2% of total radioactivity.
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