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Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases – μ-calpain and m-calpain, found in mammalian tissues. The level of components included into the calpain-calpastatin system determine the rate of post mortem tenderization of meat.In the coding region of the bovine CAST gene (CAST) the new nucleotide sequence polymorphism was found being a substitution G→C at position 61 nt within the exon 1C (consensus sequence – GenBank AF117813). This sequence fragment of SNP position has already been deposited in the GenBank database under accession no. AY258325. Consensus of bovine CAST sequence with that of human (GenBank M86257 and M28230) revealed that G→C substitution was located at position 1460 nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution at position 20 of amino acid sequence of CAST protein. The mutation creates a new AluI restriction site and,therefore, can be easily detected with PCR-RFLP.The CAST RFLP-AluI polymorphism was studied in 138 bulls of seven breeds, including the native Polish Red (PR, preserved), and Polish Black-and-White (BW) breed. The frequency of alleles C and G varied between the breeds considered, the mean reaching 0.69 and 0.31, respectively. No homozygous genotype GG was found in Red Angus, Charolaise and Hereford bulls.
The single and combined effects of polymorphism in the intron 12 of the bovine calpastatin (CAST) gene on quality traits of meat were examined in seventy-one young bulls of four beef breeds (Charolaise, Limousine, Aberdeen Angus, Hereford) and one dual purpose breed (Simmental). Three single nucleotide polymorphisms (SNP) were considered. The analysed meat traits were pH, fat content, water-holding capacity, thermal drip loss, and results of instrumental and sensory evaluation, all measured in longissimus dorsi (LD) muscle 48 or 72 h post mortem. The genetic variants at the intron 12 of the bovine CAST gene showed a marked effect on beef quality. Analyses of meat revealed differences between animals of different genotypes. Genotypes TC/TA at locus CAST/AluI-BseYI and GG at locus CAST/NdeI showed lower thermal drip loss than two remaining genotypes at each of these loci. Moreover, allele G at the polymorphic NdeI site appeared favourable for the tenderness of beef as evaluated by penetration force. In addition, significant relations were shown between combined CAST genotypes and tenderness, juiciness and flavour of meat.
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