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1

Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum

100%
Zhang Y., Hu C., Zhang C., Gan L.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 4
Based on the significant differences in the resistance to Sclerotinia sclerotiorum between two Brassica napus cultivars, ZhongR888 and Zhongyou821, near isogenic lines (NILs) were established. The resistance differences between Zhongyou821 and BC₅ progeny (the fifth generation from a cross between Zhongyou821 and ZhongR888 with Zhongyou821 as successive backcross parent) was displayed by cDNA-AFLP and differential transcripts-derived fragments (TDFs). The full-length cDNA of 1,707 bp with 1,323 bp open reading frame (ORF, open box), named Rsk was identified by RT-RACE from the resistance related fragment 40-2 in Zhongyou821. It encoded a protein of 440 amino acid residues, which contained a putative extracellular domain, a transmembrane domain, and an intracellular domain. Analysis of the deduced amino acid sequence of Rsk revealed that it had high homology to Arabidopsis thaliana kinase involved in protein binding, and had a conserved region of LRR-RI, indicating it might be a member of leucine-rich repeats, ribonuclease inhibitor-like subfamily. DNA sequences of 1,685 bp and 1,703 bp without an intron were also identified from Zhongyou821 and BC₅, respectively. Expression analysis showed that Rsk might play a role in disease resistance pathways.
2

Droxinostat sensitizes human colon cancer cells to apoptotic cell death via induction of oxidative stress

100%
Huang Y., Yang W., Zeng H., Hu C.
Cellular and Molecular Biology Letters
|
2018
|
tom 23
Upregulation of histone acetylation plays a critical role in the dysregulation of transcription. It alters the structure of chromatin, which leads to the onset of cancer. Histone deacetylase inhibitors may therefore be a promising way to limit cancer progression. In this study, we examined the effects of droxinostat on the growth of HT-29 colon cancer cells. Our results show that droxinostat effectively inhibited cell growth and colony-forming ability by inducing cellular apoptosis and ROS production in HT-29 cells. Notably, the apoptotic inhibitor Z-VAD-FMK significantly decreased the levels of cellular apoptosis and the antioxidant γ-tocotrienol (GT3) significantly decreased ROS production induced by droxinostat treatment. Z-VAD-FMK and GT3 also partially reversed the negative growth effects of droxinstat on HT-29 cells. GT3 treatment decreased cellular apoptosis and increased colony-forming ability upon droxinostat administration. Z-VAD-FMK treatment also partially decreased droxinostat-induced ROS production. Our findings suggest that the effects of droxinostat on colon cancer cells are mediated by the induction of oxidative stress and apoptotic cell death.
3

PCB biodegradation and bphA1 gene expression induced by salicylic acid and biphenyl with pseudomonas fluorescence P2W and ralstonia eutropha H850

100%
Hu C., Zhang Y., Tang X., Luo W.
Polish Journal of Environmental Studies
|
2014
|
tom 23
|
nr 5
Organic carbon substrate amendments are promising bioremediation strategies to induce polychlorinated biphenyls (PCB) aerobic degradation. However, their selective induction on PCB degraders has not been well studied. In this study, the substrate interaction effects of salicylic acid and biphenyl on PCB biodegradation were investigated with pure cultured isolates, including a newly isolated Pseudomonas fluorescence (P2W) and the veteran PCB degrader Ralstonia eutropha (H850). A significant biodegradation of lower-chlorinated PCB in H850 was induced by both salicylic acid and biphenyl amendments, while the biodegradation in P2W was induced only by salicylic acid. The binary substrates of salicylic acid and biphenyl resulted in a significantly inhibited effect on PCB removal in both strains. The expression of the functional gene bphA1 in the upper biphenyl degradation pathway was further investigated by quantitative reverse transcription PCR. Compared to H850, P2W had higher expression in the bphA1 gene induced mainly by salicylic acid rather than biphenyl. Particularly, the binary substrate induction led to an excessive expression of bphA1 gene in both strains, which was in good agreement with their biomass growth. These results suggested that the special induction of PCB biodegradation depends on the selection of organic carbon substrates and the acclimation of degrader strains.
4

Effect of NO3 −: NH4 + ratios on growth, root morphology and leaf metabolism of oilseed rape (Brassica napus L.) seedlings

76%
Qin S., Sun X., Hu C., Tan Q., Zhao X., Xin J., Wen X.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 09
5

Molybdenum-induced alteration of fatty acids of thylakoid membranes contributed to low temperature tolerance in wheat

76%
Wu S., Wei S., Hu C., Tan Q., Huang T., Sun X.
Acta Physiologiae Plantarum
|
2017
|
tom 39
|
nr 10
6

Drought stress tolerance mediated by zinc-induced antioxidative defense and osmotic adjustment in cotton (Gossypium Hirsutum)

76%
Wu S., Hu C., Tan Q., Li L., Shi K., Zheng Y., Sun X.
Acta Physiologiae Plantarum
|
2015
|
tom 37
|
nr 08
Zinc, as one of the essential microelements in crop plant, plays a crucial role in resistance to drought stress. To acquire a comprehensive of the physiological mechanism of resistance to drought stress enhanced by supplemental Zn in cotton, a hydroponic trial was conducted to investigate the changes in antioxidants and osmoregulation substances under zinc deficient and zinc sufficient conditions after 0, 3, 6 and 48 h of polyethylene glycol 6000-simulated drought stress. The present research showed that supplemental Zn significantly enhanced photosynthetic rate, chlorophyll a, chlorophyll b and dry matter of cotton under polyethylene glycol 6000 simulated drought stress, indicating that supplemental zinc improved the cotton growth. The antioxidant enzymes activities such as catalase, ascorbate peroxidase and superoxide dismutase, and non-enzymatic antioxidants such as carotenoid, reduced glutathione and ascorbic acid were all significantly enhanced and malonaldehyde content was remarkably reduced by supplemental zinc under polyethylene glycol 6000 simulated drought stress, indicating that the capacity of scavenging active oxygen species was improved by supplemental zinc in cotton. The osmoregulation substances such as soluble sugar, proline and soluble protein were all enhanced by supplemental zinc under Polyethylene glycol 6000 simulated drought stress, suggesting that zinc enhanced the osmotic adjustment capacity of cotton. It is implied that supplemental zinc could enhance the resistance to drought stress by inducing the buildup of antioxidative defences and osmotic adjustment ability in cotton.
7
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Abiotic mechanisms for biochar effects on soil N2O emission

76%
He C., Manevski K., Andersen M. N., Hu C., Dong W., Li J.
International Agrophysics
|
2019
|
tom 33
|
nr 4
8

Effects of inorganic seed aerosol on the formation of nitrogen-containing organic compounds from reaction of ammonia with photooxidation products of toluene

64%
Xu J., Huang M., Feng Z., Cai S., Zhao W., Hu C., Gu X., Zhang W.
Polish Journal of Environmental Studies
|
2020
|
tom 29
|
nr 1
9

Mass spectral analysis of the aged 1,3,5-trimethylbenzene secondary organic aerosol in the presence of ammonium sulfate seeds

64%
Huang M., Xu J., Cai S., Liu X., Hu C., Gu X., Fang L., Zhang W.
Polish Journal of Environmental Studies
|
2017
|
tom 26
|
nr 4
The ammonium sulfate ((NH₄)₂SO₄) seed aerosols commonly found in the Chinese urban atmosphere could affect the formation and aging of secondary organic aerosols (SOA). Aging of aromatic SOA is performed using UV-irradiation of 1,3,5-trimethylbenzene (135-TMB)/CH₃ONO/NO/air mixtures with high concentrations (~100 μg/m³) of (NH₄)₂SO₄ seeds in the laboratory chamber in this study. The particulate products of SOA were measured using an aerosol laser time-of-flight mass spectrometer (ALTOFMS) in real-time, and fuzzy c-means (FCM) was applied to the mass spectra organic species for clusters. Experimental results indicated that methylglyxoal, 2-methyl-4-oxo-2-pentenal, 4-methyl-1H-imidazole, 4-methyl-imidazole-2-acetaldehyde, and other imidazole derivative compounds are the principal products in the aged particles. Imidazole compounds that can absorb solar radiation effectively were newly detected in the aged 135-TMB SOA with high concentrations of (NH₄)₂SO₄ seed aerosols. These would provide new information for discussing the 135-TMB SOA aging mechanism.
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