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The educational publication contains microphotographs and description of histological preparations of the structure of marbled crayfish. The above materials may be used both for carrying out laboratory work on disciplines "Histology", "Cytology", "Cell Biology", "Special Practice", and for selfstudy of relevant educational topics. Designed for specialists in the field of hydrobiology and histology, students and graduate students of institutions of higher education who studying in the field of "091 Biology", "207 Water bioresources and aquaculture" and "162 Biotechnology and bioengineering". The publication contains the results of studies conducted by President’s of Ukraine grant for competitive projects Ф75/142 «The reproductive potential of invasive species of Dnieper region reservoirs and their impact on bioproductivity formation» (№ 0118U006319) of the State Fund for Fundamental Research.
Manganese, lead and nickel are heavy metals, which are the primary fresh water toxic contaminants being in the most dangerous class of chemicals. Heavy metals cause functional disorders in the tissues and organs of hydrobionts, affecting their linear and weight indices, reproductive system, digestive and extraction organs. In our experiment on marbled crayfish Procambarus virginalis (Lyko, 2017) we studied the long-term effects of various concentrations of heavy metal ions on the physiological state and histostructure of tissues and organs. According to the results of research, it was found that among the studied heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. The results of morphometric studies on marbled crayfish indicate no significant differences between the control group and experimental one, but heavy metals influenced the form of the hepatopancerase lobes and the size of the lumen of the gland. Research on adipocytes of the connective tissue showed a significant difference between the size of cells under the influence of heavy metals. Dimensions of adipocytes fluctuated over a wide range from 144 μm² to 537 μm². In control group the adipocyte area was 406.96 μm². Experimental studies of hemolymph showed that, under the influence of lead ions, a significant 1.4 times increase was observed in the area of hemolymph cells; when manganese and nickel were added, moderate decrease was observed in cells. It was found that under the influence of nickel, the area of round hyalinocytes has reduced by 1.7 times.
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