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Siberian sturgeons were given intraperitoneally 100 mg/kg b.m. of oxytetracycline (OTC) and 24 h after the injection they were immersed in lysozyme dimmer solution (KLP-602) at 100 µg/L of water for 30 min. The effect of OTC, applied separately and in combination with lysozyme dimer, on hepatopancreas morphology has been examined. The results of microscopic and ultrastructural examinations of the organ indicated the existence of morphological lesions following the administration of OTC: congestions and extravasations, parenchymal and lipid-related degeneration, and focal necrosis with relatively frequent infiltrations of lymphoid cells. Ultrastructural examination revealed damage to the mitochondria in hepatocytes. KLP-602 was found to have a protective effect in these changes. Immersion of the fish in an aqueous solution of lysozyme dimer was shown to have an immunomodulating effect, reducing the intensity of morphological changes that resulted from the administration of OTC.
The liver and kidneys of broilers fed during the first two weeks of fattening a fodder containing the addition of 0.025% propolis and/or 0.5% pollen were examined macroscopically and microscopically. Additionally, the effects of these substances on the results of breeding and on pathomorphology of the liver and kidneys in the course of natural, asymptomatic infection with Salmonella Enteritidis were determined. The results demonstrated that propolis has protective effects on the liver of broiler chickens, reducing the intensity of regressive lesions. However, regarding supplementation of the diet with bee pollen, additional research on its effects in animals is needed.
Considering the amendments to the regulations on animal protection in the European Union (EU), the authors outline the legal perspective on the role of veterinary surgeons in the protection of animals used in experiments. They point out that the use of animals in scientific experiments and for educational purposes has been a source of controversy, and that there have been various forms of protest against experiments that inflict pain and suffering (not only physical) on animals. Currently, the need to protect animals from pain and suffering, also in the course of scientific experiments, is a commonly accepted ethical standard. In outlining the history of the topic, the authors note that in the 20th century (from 1928 till 1997), the Polish law on animal experiments was based on two articles of a statutory instrument. This general legislation was extended in the Act on Animal Protection of 1997. After the accession of Poland to the European Union, the protection of animals used in experiments was regulated by a separate act (The Act on Experiments on Animals of 2005). This resulted from the requirements set by the Council Directive of November 24, 1986, on the approximation of laws, regulations and administrative provisions of the member states regarding the protection of animals used for experimental and other scientific purposes (86/609/EWG). Despite the substantial expansion of regulations adopted at the turn of the 21st century, the actual protection of animals used for scientific purposes raised objections in many European countries. As a result, in 2010 a new directive was issued to reinforce the legal protection of animals used for experimental and educational purposes. However, the EU legislation still leaves a great deal of freedom to the member states in constructing their own regulations. In particular, it does not specify the role of veterinary surgeons as a professional group with specialist knowledge and legal authorization in the process of approving and supervising experiments on animals. The Directive obliged the EU member states to implement the new legislation by the end of 2012. In Poland, the legislative process was initiated in January 2014. The bill prepared by the Ministry of Science and Higher Education includes regulations that contradict the intent of the Directive. The bill fails to recognize the role of veterinary surgeons, ignoring their knowledge and skills to identify pathological conditions in animals and to administer medical treatment. It is the sole professional group legally authorized to diagnose and treat animals and to perform medical procedures on animals. In addition, there is a variety of veterinary specializations, including those in the use and pathology of laboratory animals. Unfortunately, the proposed new regulations take no account of all this knowledge, experience and legal authority. It is undeniable that veterinary surgeons possess the knowledge, legal authority and a special attitude towards animals which enable them to objectively and professionally assess all stages of experiments on animals. The current proposals are insufficient to make veterinarians obligatory members of ethical commissions on animal experiments. Another inconsistency in the bill is that it does not provide for the involvement of veterinary surgeons in anesthetic procedures or at least for their supervision over such procedures (which is crucial for the protection of animals from suffering). These facts are difficult for the veterinary profession to accept. Considering the premises that form the basis for the Directive of 2010, it would be reasonable to expect that the new Polish legislation will ensure an extensive protection of animals used for experimental purposes. This requirement will not be met unless veterinary surgeons are involved in evaluating proposed experiments on animals and in direct supervision over such experiments.
According to the available literature, budgerigars are the most susceptible to being infected with avian polyomavirus (APV), whereas this infection is very rare in Amazon parrots. Although the same virus is responsible for the disease, clinical symptoms in the Amazon parrot are considerably different than those observed in budgerigars. APV is transmitted primarily bird-to-bird but it is also thought to be transmitted via the egg. Many affected young amazon die, while most infected adult birds develop lethargy, poor appetite and diarrhoea, with the surviving birds developing antibodies to the virus. However, despite the common misconception, that adult birds are more resistant, the adult amazon are readily susceptible to infection, can become ill and some may die. The main clinical symptoms of APV infection in the amazon parrot include hepatomegaly, ascites and hydropericardium. Necropsy often show spleno- and hepatomegaly with irregular red and yellow mottling of the liver, while histopathological examination present pathognomomic lesions as multifocal necrosis in the liver and kidney, enlarged nuclei and enlarged amphophilic intranuclear inclusions in the liver, kidney and spleen. Procedure against APV infection in an outbreak requires vaccinating the adults and neonates to stimulate flock immunity, as well as cleaning and disinfecting the contaminated facility.
The aim of the study was to determine the degree of conflictogenity of some animal species and to show the reasons for this conflictogenity that, in turn, result in the issuing of veterinary expert opinions. Research was carried out on 173 expert opinions, collected from the whole country, and drawn up by scientists from the Department of Forensic and Administration of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, between 1995 and 2005. In most cases (68.2%) these opinions established the actual state of the cases examined in civil proceedings. It was found that the most frequent expert opinions were issued on poultry (61.8%) and, in particular, on broilers, turkeys and hens. These opinions indicated that bacteria (mainly Escherichia coli and species of Clostridium genus) and fungi, or their toxins, were the predominant causative agents responsible for the examined losses in poultry production. It was established that breeders were to blame for these losses in only a few of the cases. The expert opinions on cattle and horses were commissioned by both insurance companies and civil courts - quite frequently veterinary surgeons were imputed. The opinions on dogs were mainly of a penal character and aimed at determining the cause of death. An analysis of opinions issued over the last ten years indicated that there had been a significant decrease in the role of infectious agents originating in the diet in instituting proceedings. Furthermore, the quality of veterinary documentation had improved only slightly.
With the development of toxicology as a science, the second half of the 20th century saw the establishment of scientific societies, such as the International Society of Ecotoxicology and Environmental Safety (SECOTOX), the Federation of European Toxicologists and European Societies of Toxicology (EUROTOX), the Polish Society of Toxicology, and the European Society of Toxicologic Pathology (ESTP). This study describes their development, organizational structure and mission. The scope of their interest, in addition to toxicology, also includes ecotoxicology, which protects the natural environment and public health.
The most popular rainbow trout (Oncorhynchus mykiss, Walbaum 1792) production technologies include both an extensive method with the flow through system (FTS) and an intensive method with the recirculating aquaculture system (RAS). Their impact on the fish was evaluated with a morphological assessment of the gills, as these are organs susceptible to environmental changes. Trout of 350 – 500 g body mass were caught for trial in spring and autumn, with 36 fish originating from 3 fish farms with the FTS system and an equal number from 3 RAS fish farms (n = 6). The fish were macroscopically examined and the gills were investigated microscopically (haematoxylin and eosin staining). Hypertrophy and hyperplasia were most commonly detected, amounting to 89% of all structural abnormalities. These lesions were slightly more common in the FTS, especially in autumn, whereas the changes to the blood vessels in the gills were observed more frequently in the rainbow trout from the RAS system than in the fish obtained from the FTS technology (the difference was statistically significant). The morphological lesions in the mucous cells of the gills were detected at a comparable severity regardless of the technology or production season. The predominantly low values of the histopathologic indices, which described the microscopic lesions in the gills of fish from the FTS and RAS systems, showed that the examined organ was most often free of lesions or demonstrated only minor morphological damage regardless of the production technology.
The current veterinary legislature on aquaculture was developed 20 years ago when the European Union consisted of only 12 Member States. It was created in order to protect the-then basic field of activity of this sector, which was the culturing of salmonids (trout and salmon) and oysters. Currently, this law requires updating so as to reflect the wide scope of activity within the aquaculture sector and species now found in the enlarged Union. It should also include significant developments in this branch and the experience gained through the last 15 years of implementing the existing law and scientific progress in this discipline. EU regulations should also be updated to conform to international agreements and norms (e.g.: WTO/SPS and OIE). The proposed new regulations submitted to the Council will abolish the present laws (Council directives 91/67/EEC, 93/53/EEC and 95/70/EC) and replace them with a new directive on health requirements for aquatic animals and products thereof, and on the prevention and control of certain diseases in these animals. This will facilitate higher flexibility and give operational responsibility to the Member States, thus enabling effective disease prevention through initiating decision-making at a local or regional level.
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