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Six-month-old, uniform-sized seedlings of Cleopatra mandarin growing in hydroponics in Hoagland nutrient solution under glasshouse conditions were subjected to salinity treatment (NaCl 75 mM). Addition of NaCl to the nutrient medium reduced plant growth (shoot height and leaf number), leaf chlorophyll content, chlorophyll fluorescence yields (Fv/Fm), net photosynthesis, stomatal conductance, transpiration rate, intracellular CO₂ concentration, N, K⁺ and Ca²⁺ + Mg²⁺ contents of the leaves; and increased total putrescine (Put), proline and Na⁺ and Cl⁻ contents of the leaves. Addition of D-arginine (1 mM) to the saline medium further reduced the values of all the parameters except Fv/Fm, photosynthesis and related parameters and Ca²⁺ + Mg²⁺ contents of the leaves, and increased total spermine (Spm) content of the leaves. However, total Put contents were decreased; spermidine (Spd) contents and Na⁺ and Cl⁻ titres of the leaves were unaffected. Weekly spray of D-arginine (5 mM) was less harmful than its addition to the nutrient medium (1 mM). Addition of 0.5 mM Spd to the medium alleviated the adverse effects of D-arginine by improving plant growth, leaf chlorophyll content, total Put, Spd, Spm, N, P, K⁺ and Ca²⁺ + Mg²⁺ contents of the leaves. Weekly spray of Spd (5 mM) was less effective than its addition in the nutrient medium (0.5 mM).
Six-months-old, uniform sized seedlings of two citrus rootstocks; Cleopatra mandarin (Citrus reshni Hort. ex Tan) and Troyer citrange (Poncirus trifoliata · Citrus sinensis) were irrigated with half-strength Hoagland nutrient solution containing 0, 40 or 80 mM NaCl for 12 weeks. Shoot height, leaf number and fresh weights of the seedlings, and relative chlorophyll contents, chlorophyll fluorescence yields (Fv/Fm), net photosynthetic and respiration rates in the leaves decreased with the incre ase in salinity level in the irrigation water. The decrease wasgreater in Troyer citrange as compared to Cleopatra mandarin. The concentrations of sugars i.e. fructose, glucose and sucrose in the leaves of Cleopatra mandarin and both leaves and roots of Troyer citrange decreased with the increase in salinity level. However, the concentrations in the roots of Cleopatra mandarin increased with the increase in salinity level. Free proline content in the leaves of Troyer citrange and root tissue of Cleopatra mandarin also increased with the increased salinity level. Among the polyamines, spermine titer increased in the leaves of both rootstocks as a response to salinity treatments. Na+ concentrations were higher in leaf and root tissue of Cleopatra mandarin, while that of Cl were higher in Troyer citrange
Freshly isolated mesophyll and suspensions-cell protoplasts of S. tuberosum cvs. Desiree and Maris Piper were cultured in different media i.e. modified MS, V-KM and MS-KM. Protoplast plating efficiencies were higher in MS-KM medium. Resulting protoplast-derived calluses were transferred either onto the medium of Bokelmann and Roest (1983) or that of Lam (1977) for shoot regeneration. Calluses derived from mesophyll cell protoplasts differentiated about 2 weeks earlier than calluses derived from suspension-cell protoplasts. Shoot initiation was also about 2 weeks earlier from calluses subcultured onto the former medium as compared to the latter.
Seeds of onion (Allium cepa) cv. Ailsa Craig were exposed to various doses of gamma radiation (0, 10, 20, 40, 80 and 100 krad) and subjected to accelerated ageing (RH 100%, 42℃) for 12 h. Radiation doses significantly affected the percentage of normal seedlings, abnormality types (%) and seedling growth. Seed viability, conductivities of seed leachates, final germination (%), germination speed and time to reach 50% germination (T₅₀) were not affected by the seed irradiation. Accelerated ageing after irradiation had significant influence on seed viability, conductivities of seed leachates, final germination (%) and percentage of normal seedlings. Germination speed, T₅₀ and seedling growth were not affected by the accelerated ageing. However, all the parameters studied were significantly influenced by the interaction of radiation doses and accelerated ageing. Accelerated ageing generally enhanced the damaging effects of irradiation on seeds. Therefore, it was concluded that onion seeds should not be exposed to adverse environmental conditions after irradiation.
Wild raspberry (Rubus idaeus L.) is abundantly grown and widely distributed in temperate regions of lower Himalayan range which represents rich biological diversity for raspberry. Thirty accessions were explored from three different locations of Azad Jammu and Kashmir (Pakistan), several traits were visualized at sampling locations and some characteristics were evaluated and quantified after harvest of leaf and fruit samples. The accessions differed greatly in growth habit, blooming time, intensity of flowering, fruit setting, ripening time, productivity, fruit shape and color. Concerning quantitative characteristics, significant differences were found in plant height, leaf length and width, number of braches per plant, duration of flowering, fruit setting percentage, days to maturity, fruit length, width and weight among the samples collected from different locations. Ripe fruits were evaluated for their chemical composition i.e. moisture, carbohydrates, protein, fat, fiber, ash, TSS, acidity, pH and mineral (K, Ca, Na, Fe, Cu, Zn, Mn, Pb, and Cr) contents. The location also had significant effect on chemical composition of ripe fruits.
Indian jujube (Zizyphus mauritiana Lamk.) is mostly cultivated in tropical and sub-tropical regions of the world. Many native and exotic jujube cultivars are grown for fruit production in Pakistan. However, little research work has been conducted on various aspects of jujube i.e. morphological and biochemical characterization of available germplasm. Therefore, fruits of thirteen genotypes were collected to study the biodiversity through physico-chemical analysis during the years 2015 and 2016. The aim of the present study was to investigate the relationship among various physico-chemical attributes of the jujube through Pearson’s correlation that may have greater importance for breeders during the selection of desirable genotype. The cultivar Foladi had the maximum fruit weight (30.49 g), pulp weight (28.42 g) and fruit diameter (36.75 mm) among all the cultivars. The maximum fruit length was recorded in Umran-13 (45.16 mm), while the maximum seed weight (2.70 g) was found in Dilbahar. Khobani had the maximum TSS (14.92 ºBrix). The highest level of acidity (0.74%) was recorded in Gorh. Akasha had highest vitamin C content (72.53 mg 100 mL–1 juice). The cultivars Sadqia, Umran-13, Mehmood wali, Yazman local and Gorh were much sweeter due to the maximum total sugars content (9.74–10.09%). The maximum antioxidant capacity was measured in Mahmood wali (616.13 mM Trolox 100 mL–1), Pak white (615.02 mM Trolox 100 mL–1) and Seedless (600.46 mM Trolox 100 mL–1), while antioxidant activity was significantly higher in Sadqia (40.604%). The maximum amount of total phenolic content was determined in Umran-13 and Sadqia (243.06 and 239.25 µg GAE mL–1 juice, respectively). The highly significant correlation (0.99) was observed between the fruit weight and pulp weight. Antioxidant activity and total phenolic content were also strongly correlated (0.70). Principal component analysis was made to determine the relationship among the genotypes and their variables. Dendrogram constructed on the basis of morphological attributes, divided 13 genotypes into four main clusters. Among the cultivars, Khobani and Mehmood wali share the maximum similarity (78%). Biochemical characteristics also divided the genotypes into three main clusters. The cultivars Pak white and Seedless had the maximum similarity (75%) among all the cultivars.
Sweet potato (Ipomoea batatas (L.) Lam) is an important crop due to its cultivation as staple food for millions of small farmers as well as for poor people in Latin America, Asia, Africa and in many other parts of the world. This tuberous crop is susceptible to drought stress especially during the period of crop establishment as well as vine development and tuber initiation. Yield of the crop vary widely among farmers due to improper planting systems. This current study was aimed to investigate the influence of various irrigation intervals and planting systems on vegetative growth, storage root yield and quality of sweet potato (Ipomoea batatas (L.) Lam) cv. ‘White star’ under field conditions. Three irrigation intervals (7, 14 and 21 days for summer crop, and 14, 28 and 42 days for winter crop) and two planting systems (bed planting and ridge planting) were adopted in this study. Vine length, number of branches and average leaf area significantly reduced as the irrigation interval was increased. Ridge planting produced longer vines with greater leaf area in winter crop as compared to bed planting. Yield parameters (storage root length, storage root diameter, number and fresh weight of marketable roots per plant) were directly linked with vegetative growth especially in summer crop. Under water stress conditions, as vegetative growth decreased storage root yield was also reduced. However, yield attributes were not affected by the planting systems. Vitamin C content decreased with water stress, whereas total soluble solids (TSS) and leaf proline content significantly increased with water stress in summer crop. Ridge planting also resulted in increased leaf proline content in summer crop. It is concluded that for attaining good vegetative growth and storage root yield, sweet potato should be irrigated at an interval of 7 days during summer and 14 days during winter crop and planted on ridges.
In the present work, genetic diversity in nutritional composition of sixteen plum genotypes growing at four different locations of Tehsil Rawalakot, District Poonch of Azad Jammu and Kashmir (Pakistan) were studied. Various parameters like moisture, dry matter, ash and total soluble solids contents, acidity, pH, vitamin C and sugar content, shelf-life and sensory/organoleptic evaluation, anthocyanins, phenolics and antioxidant activity were evaluated and variation in these characteristics has been discussed. The results suggested that the genotypes differed in their nutritional composition of fruits, anthocyanin and phenolic contents and antioxidant activity of fruit. The results of the present study regarding the nutritional status of existing plum germplasm will contribute and increase our knowledge about the genus Prunus and broaden the gene pool available for future plant breeding programs.
A field study was conducted to evaluate the effect of different plant growth regulators (PGRs) applied through seed priming and foliar spray on growth and yield of three okra cultivars grown under calcareous soils. The cultivars of Punjab Selection and Sabzpari produced significantly higher number of branches and leaves per plant, pod length and diameter and pod yield as compared to cv. Green ferry, while, cv. Punjab selection produced significantly greater number of flowers and pods per plant as compared to other two cultivars. Seed germination (%), plant height, and fruit set (%) were not affected by the cultivars. Among the PGR treatments, seed primed with GA3 resulted in significantly higher germination percentage and greater plant height at flowering. Seed priming and foliar spray with NAA and GA3 were effective in increasing the final plant height, number of branches, number of leaves, number of flowers and number of pods per plant and fruit set (%), fresh weight per pod and pod yield. However, pod diameter, pod moisture content and dry weight per pod were not influenced by the PGR treatments applied. These results suggested that the PGRs have great potential to improve seed germination, enhance growth and increase yield of okra cultivars under calcareous soils.
Different physiological behavior of a wide range of varieties and species belonging to the Citrus genus was analyzed when subjected to salt stress with the aim to seek new sources of tolerance that might be specie-specific. Our goal was to use physiological results obtained along a salt stress in order to clarify if it would be possible to associate them with the known citrus genetic diversity. For that purpose, we have selected 20 different genotypes representing the major species on the basis of the genetic diversity of Citrus genus complemented with one intergeneric hybrid Carrizo citrange (C. sinensis 9 P. trifoliata). A moderate salt stress of 75 mM of NaCl was applied for 12 weeks. For control plants, the main parameters contributing for more than 25 %to the diversity on the two axes of principal component analysis (PCA) were chlorophyll content, photosynthesis and Fv/Fm under light. However, the dispersal of species and varieties on the PCA did not show any particular structure. Under salt stress condition, four parameters (leaf chloride content, leaf chlorophyll content, photosynthesis and stomatal conductance) contributed more specifically to the dispersion on PCA representation with more than 15 % of contribution for each parameter. Large differences were observed within citrus genus: mandarin and pummelo presented good tolerance to salt stress while citron was very sensitive. Furthermore, all secondary genotypes that presented good tolerance to salt tolerance shared mandarin or pummelo as female parent.
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