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The present study was undertaken to determine the aflatoxin M₁ (AFM₁) levels in various cheese samples in Istanbul. A total of 363 samples of cheese, 131 samples of white cheese, 132 samples of processed cheese, 100 samples of kashar cheese brought to the Istanbul Military Food Control Laboratory were analysed from 2002 to 2004. The quantitative detection of AFM₁ levels in the samples was performed by competitive enzyme linked immunoassay (ELISA) method. AFM₁ levels in 283 (77.96%) of 363 cheese samples were found to be lower than 0.25 µg/kg, determined as the maximum acceptable limit by the Turkish and International Standards and 80 (22.04%) of those were higher. The samples of unacceptable level rates were also quite different depending on the years. The highest rate (40.19%) was determined in 2002 and the rates in 2003 and 2004 were 15.39% and 13.49%, respectively. The results have indicated that high level of AFM₁ in cheese samples can provide a potential risk for consumer health, but the decreasing level of AFM₁ in the latest years may be evaluated as positive progress.
The effect of chitosan in combination with polyphenol oxidase (PPO) inhibitors and other additives (citric acid, rosemary extract) to inhibit or slow down melanosis during chilled and frozen storage was investigated. Fresh deepwater pink shrimps (Parapenaeus longirostris) were dipped in different solutions containing combinations of sodium metabisulphite (2500 mg/L), 4-hexylresorcinol (50 mg/L), chitosan (5 g/L), citric acid (200 mg/L) and rosemary extract (50 mg/L) and stored at 4°C for six days and at –18°C for twelve months. During storage, changes in colour were defined sensorially and instrumentally. Chitosan alone showed no effect on discoloration, but in combination with PPO inhibitors showed an additional inhibitory effect on melanosis formation. No noticeable darkening was observed during frozen storage in all treated shrimps, whereas signs of blackening were noticeable from the third month; 50 mg/L of 4-hexylresorcinol were more effective than 2500 mg/L of sodium metabisulphite in the prevention of melanosis development after shrimping.
The aim of the study was to investigate the effectiveness of various disinfectants on Salmonella enteritidis inoculated on eggshells. The contaminated eggs were treated with two different disinfectant solutions (benzalkonium chloride (BAG) and benzalkonium chloride / gluteraldehyde combination (BAC/G)) for 5 and 15 minutes. Following the treatment and storage at room temperature, the shells and their content were examined for S. enteritidis on days 7, 14, and 21. The results indicate that S. enteritidis may remain viable on the shells of non-disinfected eggs for a long period of time, and may penetrate into the edible portions of the egg during this period. Treating the eggs with a combination of benzalkonium chloride / gluteraldehyde for 15 minutes may probably safely eliminate the danger of S. enteritidis contamination.
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