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Effects of Cu2plus, cytokinins and jasmonate on content of two flavonols identified in zucchini cotyledons

100%

Stoynova-Bakalova E., Nikolova M., Maksymiec W.

Acta Biologica Cracoviensia. Series Botanica

2009

tom 51

nr 2

77-83

This study examined the effects of cytokinins and methyl jasmonate (JAMe) alone and in combination on the growth and flavonol content of zucchini cotyledons cultured in the presence or absence of Cu2+. During greening of cotyledons in intensive diurnal illumination, rutin was identified as the main flavonol compound. Its accumulation was greatly stimulated by phenylurea cytokinin (4PU-30) but reduced by Cu2+. Stimulation by benzylaminopurine (BA) was less. JAMe showed an inhibitory effect, alone and with simultaneous addition of Cu2+ or cytokinins. In moderate excess (100 μM and 250 μM CuSO4), Cu2+ enhanced stimulation by 4PU-30 of rutin accumulation; at a higher concentration or with other substances it decreased the rutin level. The other flavonol detected, kaempferol-3-rhamnoside, increased after JAMe or 4PU-30 were added; in the other cases it decreased. The data obtained indicate that cytokinins can increase rutin content in developing Cucurbita cotyledons. Rutin content usually decreased under stress induced by excess Cu2+, but Cu2+ in moderate excess had a stimulating effect in the presence of higher phenylurea cytokinin levels

Cytokinin oxidase-dehydrogenase [CKX] activity in wild and ethylene-insensitive mutant eti5 type of Arabidopsis thaliana [L.] Heynh plants and the effect of cytokinin N1-[2-chloro-4-pyridyl]-N3-phenylurea on enzymatic activity and leaf morphology

63%

Todorova D., Vaseva-Gemisheva I., Petrov P., Stoynova-Bakalova E., Alexieva V., Karanov E., Smith A., Hall M.

Acta Physiologiae Plantarum

2006

tom 28

nr 6

The specific activity of cytokinin oxidase/dehydrogenase (EC 1.5.99.12) (CKX) was determined in leaves of wild type (wt) and ethylene-insensitive mutant (eti5) of Arabidopsis thaliana (L.) Heynh plants. Comparative studies showed that this mutation has lower basal CKX activity than wt. Application of 4PU-30 (N1-(2-chloro-4-pyridyl)-N2-phenylurea) resulted in decreased CKX activity in both wt and mutant plants. The treatment increased leaf blade thickness and the volume of chlorophyll-containing cells per unit leaf area in wt but these changes were not observed in the eti5 mutant. The reduction in chlorophyll “a” and “b”, as well as in carotenoids content in the treated wt tissues resulting from altered leaf morphology was not detected in eti5 plants.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Physiologiae Plantarum

2 Stoynova-Bakalova E.

1 Alexieva V.

1 Hall M.

1 Karanov E.

1 Maksymiec W.

1 Nikolova M.

1 Petrov P.

1 Smith A.

1 Todorova D.

1 Vaseva-Gemisheva I.

1 2009

1 2006

Wyniki wyszukiwania

Effects of Cu2plus, cytokinins and jasmonate on content of two flavonols identified in zucchini cotyledons

Cytokinin oxidase-dehydrogenase [CKX] activity in wild and ethylene-insensitive mutant eti5 type of Arabidopsis thaliana [L.] Heynh plants and the effect of cytokinin N1-[2-chloro-4-pyridyl]-N3-phenylurea on enzymatic activity and leaf morphology