Wyniki wyszukiwania

1

microRNAs fine tuning of erythropoiesis

100%

Listowski M. A., Heger E., Boguslawska D. M., Machnicka B., Kuliczkowski K., Leluk J., Sikorski A. F.

Cellular and Molecular Biology Letters

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2013

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tom 18

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nr 1

Cell proliferation and differentiation is a complex process involving many cellular mechanisms. One of the best-studied phenomena in cell differentiation is erythrocyte development during hematopoiesis in vertebrates. In recent years, a new class of small, endogenous, non-coding RNAs called microRNAs (miRNAs) emerged as important regulators of gene expression at the post-transcriptional level. Thousands of miRNAs have been identified in various organisms, including protozoa, fungi, bacteria and viruses, proving that the regulatory miRNA pathway is conserved in evolution. There are many examples of miRNA-mediated regulation of gene expression in the processes of cell proliferation, differentiation and apoptosis, and in cancer genesis. Many of the collected data clearly show the dependence of the proteome of a cell on the qualitative and quantitative composition of endogenous miRNAs. Numerous specific miRNAs are present in the hematopoietic erythroid line. This review attempts to summarize the state of knowledge on the role of miRNAs in the regulation of different stages of erythropoiesis. Original experimental data and results obtained with bioinformatics tools were combined to elucidate the currently known regulatory network of miRNAs that guide the process of differentiation of red blood cells.

2

Mutacje w genach kodujacych bialka blony erytrocytu przyczyna dziedzicznych anemii hemolitycznych

100%

Hanus-Lorenz B., Lorenz M., Boguslawska D., Heger E., Hryniewicz A., Sikorski A. F.

Kosmos

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2001

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tom 50

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nr 3

325-333

3

The kinetics of haemolysis of spherocytic erythrocytes

100%

Pazdzior G., Langner M., Chmura A., Boguslawska D., Heger E., Chorzalska A., Sikorski A. F.

Cellular and Molecular Biology Letters

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2003

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tom 08

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nr 3

Spherocytosis is a hereditary disease. It results from mutations in genes that encode proteins participating in the attachment of the membrane skeleton to the plasma membrane bilayer of the erythrocyte. In affected cells, interaction between the spectrin-actin meshwork and integral membrane proteins is altered. This results in the weakening of plasma membrane mechanical resistance and diminishing its elasticity. Since defective cells are prone to mechanical destruction and phagocytosis in the spleen, the fraction of morphologically-altered erythrocytes is rather small; this in turn means such an examination is prone to errors. In this paper, we describe a simple method which could be useful in the identification of red blood cells with altered osmotic properties. The method is based on the measurement of the amount of light scattered by a suspension of the red blood cells, during which cells are exposed to osmotic stress in the stopped-flow regime. The obtained plots are fitted to a mathematical formula, the parameters of which can be used as quantitative indicators of the changes in red blood cells' osmotic features. Two types of spherocytotic samples were examined: those with a proven deficiency in ankyrin and those with a decrease in the band 3 anion transporting protein. The presented data show that this method gives a reliable indication of altered osmotic properties of the spherocytic cells.

4

Identification of several families with hereditary spherocytosis in a population from South-Western Poland

84%

Boguslawska D. M., Heger E., Chorzalska A., Nierzwicka M., Holojda J., Swiderska A., Straburzynska A., Chmura A., Langner M., Sikorski A. F.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr Suppl.

Ograniczanie wyników

3 Cellular and Molecular Biology Letters

1 Kosmos

4 Heger E.

4 Sikorski A. F.

2 Boguslawska D.

2 Boguslawska D. M.

2 Chmura A.

2 Chorzalska A.

2 Langner M.

1 Hanus-Lorenz B.

1 Holojda J.

1 Hryniewicz A.

1 Kuliczkowski K.

1 Leluk J.

1 Listowski M. A.

1 Lorenz M.

1 Machnicka B.

1 Nierzwicka M.

1 Pazdzior G.

1 Straburzynska A.

1 Swiderska A.

1 2013

1 2003

1 2002

1 2001

microRNAs fine tuning of erythropoiesis

Mutacje w genach kodujacych bialka blony erytrocytu przyczyna dziedzicznych anemii hemolitycznych

The kinetics of haemolysis of spherocytic erythrocytes

Identification of several families with hereditary spherocytosis in a population from South-Western Poland