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1

First insight into microbial community composition in a phosphogypsum waste heap soil

100%
Zielinska S., Radkowski P., Ossowski T., Ludwig-Galezowska A., Los J. M., Los M.
Acta Biochimica Polonica
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2017
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tom 64
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nr 4
2

Activities of synthetic peptides against human pathogenic bacteria

100%
Bogucka K., Krolicka A., Kamysz W., Ossowski T., Lukasiak J., Lojkowska E.
Polish Journal of Microbiology
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2004
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tom 53
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nr 1
The increasing, problem of antibiotic resistance among pathogenic bacteria requires development of new antimicrobial agents. Synthesis and experimental application of the hybrids peptides may be one of the interesting possibilities in antimicrobial treatment. The aim of the present investigation is to determinate in vitro activities of two synthetic peptide amides: cecropin-melittin hybrid peptide (CAMEL) and protegrin analogue (IB-367) against control strains and multi-resistant clinical isolates. Antimicrobial activities were measured by MIC and MBC. The tested strains were susceptible to the peptides at concentrations in the range of 1 to 32 ug ml⁻¹.
3

Determination of volatile fatty acids in environmental aqueous samples

84%
Siedlecka E. M., Kumirska J., Ossowski T., Glamowski P., Golebiowski M., Gajdus J., Kaczynski Z., Stepnowski P.
Polish Journal of Environmental Studies
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2008
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tom 17
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nr 3
351-356
Volatile fatty acids (VFAs) are present in environmental waters in the range of 1 to 5,000 ppm and different methods have been reported for their determination. In this paper we have studied and compared analytical performance parameters for the distillation method followed by potentiometric titration, spectrophotometric and gas chromatographic methods. The main disadvantage of the distillation approach was quite poor absolute recovery (53-58%) from the given matrix and rather elevated limit of quantification (LOQ) at 110 mg/L. Direct potentiometric titration was characterized by acceptable accuracy (above 97%) and precision in the range 1.8%-15%. The LOQ value was 11 mg/L. The spectrophotometric method was sensitive for hydrogen carbonate alkalinity and phosphate ions; measured concentrations of acetic acid were lower than nominal. The precision and accuracy of the spectrophotometric method were in the ranges 1.3-14% and 82.1-104.2%, respectively. Limit of quantification was 28 mg/L. However, if ion exchange bed is used prior to this method the LOQ can be reduced to 5 mg/L. The GC method is characterized by quite low LOQ (5 mg/L) and seems to be the best methodology to determine low VFA concentrations in environmental waters. The precision of the method ranged from 5.7 to 14.8% and accuracy was above 92%. Additionally, this method allows for determination of individual VFAs.
4

Molecular modeling of singlet-oxygen binding to anthraquinones in relation to the peroxidating activity of antitumor anthraquinone drugs

84%
Liwo A., Jeziorek D., Ossowski T., Dyl D., Tempczyk A., Tarasiuk J., Nowacka M., Borowski E., Woznicki W.
Acta Biochimica Polonica
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1995
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tom 42
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nr 4
Anthraquinone derivatives are important anti-cancer drugs possessing, however, undesirable peroxidating and, in consequence, cardiotoxic properties. This results from the mediation by these compounds of the one-electron reduction processes of the oxygen molecule, which produces the highly toxic superoxide anion radical and other active oxygen species. This article summarizes the results of our studies on the molecular aspects of the mechanism of anthraquinone-mediated peroxidation which were carried out using enzymatic-assay, electrochemical, and quantum-mechanical methods.
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