Wyniki wyszukiwania

1

Contribution of genomics to the understanding of physiological functions

100%

Hocquette J. F., Cassar-Malek I., Scalbert A., Guillou F.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 3

5-16

2

Functional genomics and new markers for beef production – minireview

100%

Hocquette J.-F., Cassar-Malek I., Bernard-Capel C., Picard B.

Animal Science Papers and Reports

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2009

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tom 27

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nr 4

Beef is characterized by a high and uncontrolled variability of its quality which is one reason for the dissatisfaction of consumers. Therefore, the beef industry is asking for muscular markers to predict the ability of animals to produce high quality beef. Thanks to the development of genomics, some recent research allowed the identification of markers for muscle growth potential of bovines, for marbling and tenderness of beef and for traceability of grass-based systems. New tools (DNA or protein chips) are in development to assess a great number of these markers simultaneously.

3

The potential benefits of genetics and genomics to improve beef quality - a review

88%

Hocquette J.-F., Renand G., Leveziel H., Picard B., Cassar-Malek I.

Animal Science Papers and Reports

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2006

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tom 24

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nr 3

4

Validation of a dot-blot quantitative technique for large scale analysis of beef tenderness biomarkers

76%

Guillemin N., Meunier B., Jurie C., Cassar-Malek I., Hocquette J.-F., Leveziel H., Picard B.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 3

91-97

5

Pasture-feeding of Charolais steers influences skeletal muscle metabolism and gene expression

76%

Cassar-Malek I., Jurie C., Bernard C., Barnola I., Micol D., Hocquette J.-F.

Journal of Physiology and Pharmacology. Supplement

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2009

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tom 60

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nr 3

83-90

6

A cDNA macroarray resource for gene expression profiling in ruminant tissues involved in reproduction and production (milk and beef) traits

52%

Bernard C., Degrelle S., Ollier S., Campion E., Cassar-Malek I., Charpigny G., Dhorne-Pollet S., Hue I., Hocquette J.-F., Le Provost F., Leroux C., Piumi F., Rolland G., Uzbekova S., Zalachas E., Martin P.

Journal of Physiology and Pharmacology. Supplement

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2005

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tom 56

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nr 3

215-224

cDNA arrays have proven to be useful tools to screen gene expression in many animal species including livestock species. A collaborative program was launched to construct a ruminant cDNA collection, representative of three tissues: Muscle, Embryo and Mammary gland, named MEM. This collection gathers clones mainly arising from 3 non-normalised cDNA libraries: a directed bovine muscle library, a 14-day-old bovine embryo library and a goat lactating mammary library. It is made up of 1896 clones (637 muscle, 882 embryo and 377 mammary cDNAs), selected after sequencing and bioinformatic analyses. Amplification products yielded from these clones as well as controls were printed onto Nylon membranes to generate macroarrays. Hybridisation with relevant cDNA targets allowed checking the location of about 50 cDNAs and the specificity of each sub-set of the repertoire. Macroarrays were hybridised with radiolabelled cDNA complex targets from five different tissues (muscle, embryo, mammary gland, adipose tissue and oocyte). Both somatic and germinal complex targets gave valid hybridisation signals with 45 to 80% of the printed probes. This specific cDNA collection now provides a powerful tool for transcriptomic studies with the ultimate objective to better understand physiological and metabolic functions in ruminants. It will be subsequently included into a forthcoming larger collection.

Ograniczanie wyników

Contribution of genomics to the understanding of physiological functions

Functional genomics and new markers for beef production – minireview

The potential benefits of genetics and genomics to improve beef quality - a review

Validation of a dot-blot quantitative technique for large scale analysis of beef tenderness biomarkers

Pasture-feeding of Charolais steers influences skeletal muscle metabolism and gene expression

A cDNA macroarray resource for gene expression profiling in ruminant tissues involved in reproduction and production (milk and beef) traits