Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 11

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
High ozone concentration generates oxidative stress in plants. To investigate the detailed transcriptional regulation of Arabidopsis thaliana genes encoding antioxidant enzymes upon ozone stress, we performed a microarray analysis using Affymetrix GeneChip technology. Our transcription profiling revealed a differential expression equal or greater than 2-fold change for 2385 genes (at confidence 99%) in response to 350 ppb ozone dose after 3 and 6 hours of treatment. Among these, we chose 38 genes to be oxidative stress related in ozone treatment: 29 of them were 2 times up-regulated and 9 were shown to be down-regulated in at least one of the time points. Our study revealed a new transcription pattern for catalase genes and showed the first detailed transcriptional analysis of phenylopropanoid-related genes in ozone stress conditions.
Genome analyses in model species have provided value in biological research. As it has become clear that coding gene sequences are well conserved among genera and even tribes, comparative mapping makes it possible to transfer the information on chromosome structure and gene organization from species with well-developed genetic maps to species where such information is scarce. Furthermore, genome comparisons on the basis of a well characterized model genome in relation to more complex genomes in crop plants, offer new information about the mechanisms responsible for the evolution of chromosomal structure. Among dicotyledonous plants, A. thaliana plays the role of a model plant, especially for closely related crop species of the genus Brassica (cruciferous oilseeds and a variety of leafy vegetables). The A. thaliana genome project provides an opportunity of systematic and large-scale identification and isolation of Brassica genes, and to a better understanding various aspects of the Brassica biology and, eventually, of its breeding problems. This aspects is demonstrated on an example of the A. thaliana gene families coding for ACC syntases and oxidases, key enzymes in ethylene biosynthesis. Further progress in the analysis of A. thaliana and rice genomes should establish the synteny with many related crop species by means of detailed comparative mapping. This may greatly facilitate gene identification and studies on their organization within the genome.
AtDeg2 is a chloroplast protein with dual protease/chaperone activity. Since data on how the individual activities of AtDeg2 affect growth and development of Arabidopsis thaliana plants is missing, two transgenic lines were prepared that express mutated AtDeg2 versions that have either only protease or chaperone activity and a comprehensive ontogenesis stage-based study was performed comprising wild type (WT) plants and insertional mutants that do not express AtDeg2, as well as the two transgenic lines. The repression of both AtDeg2 activities in deg2-3 mutants altered just a few phenotypic traits including the time when cotyledons were fully opened, the time when 10% flowers were open as well as the number of inflorescence branches and seed length in plants which have completed their generative development. It was demonstrated that complete opening of cotyledons as well as the number of inflorescence branches and seed length in plants which have completed their generative development required involvement of both AtDeg2 activities, whereas the time when 10% of flowers were open was controlled by AtDeg2 protease activity. These results show for the first time that the chaperone activity of AtDeg2 is needed for some elements of generative development of A. thaliana plants to proceed normally. So far, the chaperone activity of AtDeg2 was confirmed based on in vitro assays only.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.