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Bacterial infection of the leptomeninx usually occurs as a meningocerebral form with intensive involvement of the leptomeninx. The purpose of this study was to present leptomeningitis caused by Staphylococcus aureus in geese. The geese showed the following nervous symptoms: limb paralysis, unsteady gait, unnatural posture. The study was performed on 36 dead White Italian geese 2 to 6 weeks of age from 5 breeder flocks. The geese were sectioned. The microbiological investigations of their internal organs (liver, spleen, lungs), tibiotarsal joints and brain were then performed. The bacteriological culture was incubated under oxygen conditions at the temperature of 37°C for 24 hours. The identification of the isolated bacteria was carried out using ID32 STAPH (bioMerieux). The isolated bacteria were also subjected to tests for catalase, coagulase, clumping factor, protein A and capsule polysaccharides (Pastorex Staph Plus -Bio-Rad). The brain samples were fixed in 10% formalin, embedded in paraffin and then stained with hematoxylin and eosin (H-E). The leptomeningitis was present in all geese. Meningeal blood vessels were dilated and congested, filled with erythrocytes. In the leptomeninx, abundant inflammatory cells, such as lymphocytes, heterophils, glial cells, fibroblasts and fibrin threads, were found. In some geese perivascular cuffs of inflammatory cells were observed in the cerebral cortex. The bacteriological examination of internal organs, tibiotarsal joints and the brain of the geese revealed catalase- and coagulase-positive Staphylococcus aureus. The identification of Staphylococcus aureus was confirmed with the Pastorex Staph-Plus kit. The biological test for Derzsy disease was negative.
The diagnostics of the Trypanosoma sp. invasion by means of the classic methods i.e. the methods of thin smears or thick drop or even the microhematocrite method, especially when intensity of infection is low, is very difficult. In our climatic zone, trypanosomosis is usually considered as an exotic disease. An opportunistic model of the infection with the parasite and a lack of current data on the prevalence of T. theileri in the cattle in Poland cause that it is neglected as a potential reason of contamination of tissue cultures in cattle. We showed the presence of T. theileri in culture of isolated lymphocytes from one of six heifers examined. It seems that the prevalence of the invasion of the parasite is not very intense but it should be considered as a possible threat for bovine cell culture. It is also worth including this parasitosis in the differential diagnostics of other diseases that are infectious and/or proceed with symptoms of immunosuppression.
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