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Prevalence of babesiosis (Babesia bovis and Babesia bigemina) in cattle and water buffalo in Nueva Ecija, Philippines using Nested Polymerase Chain Reaction

100%

Herrera P. C. T., Viloria V. V., Balbin M. M., Mingala C. N.

Annals of Parasitology

2017

tom 63

nr 4

The study was conducted to determine the prevalence of Babesia bovis and Babesia bigemina infection in blood samples of cattle and water buffaloes using nested polymerase chain reaction (nested-PCR). It also aimed to generate a spot map showing areas in Nueva Ecija, the Philippines where B. bovis and B. bigemina were detected. Whole blood samples of cattle (148) and water buffalo (65) were collected for DNA extraction and subsequent nested-PCR to detect B. bovis and B. bigemina. To further confirm and validate the nested-PCR results, three selected positive samples for each B. bovis and B. bigemina were sequenced and examined for homology analysis. The results showed that the prevalence of B. bovis, B. bigemina and mixed infection in cattle were 11.49% (17/148), 10.81% (16/148) and 5.41% (8/148), respectively. Homology analysis of nucleotide sequence of three selected DNA samples for each B. bovis showed two 99% and one 96% (partial sequence analysis) identities with B. bovis Thailand strain, while B. bigemina positive samples showed all 100% identities with B. bigemina Philippine strain. The result did not demonstrate in all water buffalo samples. These findings provide information about the prevalence of B. bovis and B. bigemina in cattle and water buffaloes in Nueva Ecija, which can be beneficial for strategic planning, disease management, and control and prevention.

Characterization of drug resistance-associated TevAT1 gene of Trypanosoma evansi from Philippine water buffaloes (Bubalus bubalis)

88%

Mingala C. N., Pasag A. C. P., Salinas M. B. S., Balbin M. M., Villanueva M. A.

Annals of Parasitology

2019

tom 65

nr 4

This study detected and characterized the TevAT1 gene of Trypanosoma evansi isolates from Philippine water buffaloes (Bubalus bubalis). A total of 68 blood samples from Philippine water buffaloes were subjected to DNA extraction and PCR assay was performed using RoTat 1.2 gene to detect T. evansi. Those samples positive for T. evansi subsequently underwent another PCR assay to detect the presence of TevAT1 gene. Trypanosoma evansi was detected in 26.47% (18/68) blood samples in which distributed throughout the main islands of the country (4 from Luzon, 2 from Visayas and 12 from Mindanao). However, only 10 of these samples were positive for TevAT1 gene. Sequence alignment of the TevAT1 gene from local isolates showed no single nucleotide polymorphisms when compared to other strains in various countries. Those T. evansi without the gene of interest could be possibly resistant to some trypanocidal drugs but this needs to be further investigated in-vitro or in-vivo.

Ograniczanie wyników

2 Annals of Parasitology

2 Balbin M. M.

2 Mingala C. N.

1 Herrera P. C. T.

1 Pasag A. C. P.

1 Salinas M. B. S.

1 Villanueva M. A.

1 Viloria V. V.

1 2019

1 2017

Wyniki wyszukiwania

Prevalence of babesiosis (Babesia bovis and Babesia bigemina) in cattle and water buffalo in Nueva Ecija, Philippines using Nested Polymerase Chain Reaction

Characterization of drug resistance-associated TevAT1 gene of Trypanosoma evansi from Philippine water buffaloes (Bubalus bubalis)