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Co-localisation of nos- and chat-immunoreactivity in the spinal autonomic nuclei of the pig. An immunocytochemical study

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Calka J., Zalecki M., Arciszewski M. B.

Bulletin of the Veterinary Institute in Puławy

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2008

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tom 52

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nr 4

Our previous studies revealed an overlap of cholinergic and NO-ergic preganglionic neurons in the autonomic sympathetic and parasympathetic spinal cord nuclei of the pig. A close topographical as well as morphological relationship between both neuronal populations suggests a possible co-expression of the ChAT and NOS in the same autonomic neurons. Therefore, the goal of the present study was to determine the correlation between the ChAT- and neuronal NOS-immunoreactivity in the thoracic intermediolateral and sacral intermediolateral and intermediomedial nuclei. Double immunocytochemical staining revealed that many of the ChAT positive cell bodies demonstrated immunoreactivity to NOS. The number of the double stained cell bodies depended on the cross-section level. In the intermediolateral nucleus of the thoracic spinal cord about 60% of the ChAT-positive cells co- expressed also NOS, while in the intermediolateral nucleus of the S2 spinal segment constituted approximately 52%. The colocalisation of both substances in the intermediomedial nucleus varied from 39% at SI level until 58% at S2 spinal plane. Our data provide morphological evidence confirming the co-expression of NOS in the spinal sympathetic and parasympathetic cholinergic neurons and suggest that NO may play a role in the modulation of the spinal cholinergic transmission.

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Distribution and morphology of the NOS-immunoreactive neurons in the thoracolumbar and sacral spinal cord of the pig

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Calka J., Zalecki M., Wasowicz K., Lakomy M.

Medycyna Weterynaryjna

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2006

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tom 62

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nr 10

1134-1138

The study investigated the distribution and morphology of nitric oxide synthesis-immunoreactive neuronal cell bodies and processes in the thoracolumbar and sacral spinal cord of sexually immature gilts. Investigations revealed the following: NOS-immunoreactive fibers and singular cell bodies in regions of the dorsal horn including superficial laminae I and II and deeper laminae III and IV; prominent NOS-immunolabeled perikarya in intermediolateral nucleus of the thoracolumbar spinal segments; NOS-positive perikarya in lamina X along the thoracic, lumbar and sacral divisions of the cord; NOS-positive perikarya and fibers in the area near to the location of the intermediomedial and intermediolateral nuclei in the sacral segments of the cord. The obtained morphological results indicate that the general distribution pattern of NOS-positive neurons in the spinal cord of pigs resembles that of other species. The concentration of NO-ergic neurons in the autonomic nuclei, dorsal horn laminae I, II, III, IV and lamina X suggests a prominent role of NO-ergic neurons in visceral and sensory functions.

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Evidence for coexistence of choline acetyltransferase (ChAT)- and calcitonin gene-related peptide (CGRP)-immunoreactivity in the thoracolumbar and sacral spinal cord neurons of the pig

100%

Calka J., Franke-Radowiecka A., Zalecki M., Lakomy M.

Polish Journal of Veterinary Sciences

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2009

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tom 12

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nr 1

Previous immunocytochemical studies provided conflicting data concerning occurrence of the CGRP-immunoreactive neuronal cell bodies in the porcine spinal cord. In the present study, we have investigated expression of the CGRP and its possible coexpression with ChAT in the gray matter of the thoracic, lumbar and sacral spinal cord of the pig. Our study revealed a large number of CGRP-immunoreactive cells in the motor nucleus of the ventral horn, and less and singe perikarya intermediolateral and intermediomedial nuclei, respectively. Double staining immunocytochemistry, depending on the cross-section level, disclosed the highest ChAT/CGRP colocalization subsequently in the motor nucleus of the ventral horn, then in the intermediolateral and intermediomedial nucleus. Our data provide morphological evidence confirming expression of CGRP in the porcine spinal nuclei while its coexpression in cholinergic neurons suggests that CGRP may play a role in modulation of the spinal cholinergic transmission.

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Distribution and chemical coding of urinary bladder apex-projecting neurons in aorticorenal and testicular ganglia of the male pig

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Pidsudko Z., Listowska Z., Franke-Radowiecka A., Klimczuk M., Zalecki M., Kaleczyc J.

Polish Journal of Veterinary Sciences

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2019

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tom 22

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nr 2

5

Enteric nervous system in the European beaver (Castor fiber) pylorus–an immunohistochemical study

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Zalecki M., Makowska K., Gizejewski Z., Klimczuk M., Franke-Radowiecka A., Kasica-Jarosz N., Sienkiewicz W.

Polish Journal of Veterinary Sciences

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2019

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tom 22

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nr 1

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Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C

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Mogielnicka-Brzozowska M., Dziekonska A., Strzezek R., Zalecki M., Majewska A., Tolscik K., Kordan W.

Bulletin of the Veterinary Institute in Puławy

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2014

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tom 58

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nr 1

Sperm surface binding sites for non-zinc-binding proteins (nZnBPs) and zinc-binding proteins (ZnBPs) were studied by the fluorescence technique with biotin-labelled proteins. The nZnBPs binding pattern was unspecific, no characteristic sites on plasmalemma were found. ZnBPs were attached mainly to the acrosomal region of sperm head and to the sperm flagellum. ZnBPs added to the incubation mixture of the canine spermatozoa allowed the preservation of higher values for total motility, progressive motility, curvilinear line velocity, straight line velocity, and beat cross frequency (P < 0.05), both at time 0 and after 1 h incubation at 5°C. The addition of nZnBPs to the incubation mixture caused only weak positive effects when compared with control sample (PBS). A higher percentage of canine-ejaculated spermatozoa with intact membranes were observed when ejaculate was incubated with ZnBPs in comparison to control sample stored with PBS (P < 0.05) or nZnBPs (P < 0.05). Spermatozoa diluted with ZnBPs and nZnBPs exhibited a higher percentage of cells with active mitochondria when compared with control, both at time 0 and after 1 h; however, no statistical differences were observed. Our results emphasise the role of seminal plasma protein in securing the correct quantity and availability of zinc ions as a component regulating the motility of canine spermatozoa. The protective effect of ZnBPs against the cooling effect may be due to their ability of preventing sperm membrane damage.

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Changes in the expression of galanin and galanin receptors in the wall of the colon in pigs experimentally infected with Brachyspira hyodysenteriae

64%

Wasowicz K., Podlasz P., Chmielewska M., Losiewicz K., Kaleczyc J., Zmudzki J., Zalecki M., Pidsudko Z., Lakomy M.

Bulletin of the Veterinary Institute in Puławy

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2014

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tom 58

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nr 1

The expression of galanin (GAL) and its three receptors (GalR1, GalR2, and GalR3) were studied with real-time PCR in the colonic wall of pigs suffering from experimental colitis caused by the infection with Brachyspira hyodysenteriae. The expression was studied in the muscular membrane, mucosa/submucosa layer, and in lymphocytes isolated from mucosa/submucosa. The expression levels were normalized to glyceraldehyde-6-phosphate dehydrogenase (GAPDH) expression and compared to expression levels in control animals. GAL expression was found in all three studied compartments of the colonic wall. A significant decrease in GAL expression level was found in the mucosa/submucosa and in isolated lymphocytes, whereas the decrease was much less profound in the muscular membrane. In the case of galanin receptors their expression was found in all studied compartments of the colonic wall, however at different levels, as compared to GAPDH expression. The decrease of galanin receptors expression was found in all studied compartments of the colonic wall of the sick animals.

Ograniczanie wyników

Co-localisation of nos- and chat-immunoreactivity in the spinal autonomic nuclei of the pig. An immunocytochemical study

Distribution and morphology of the NOS-immunoreactive neurons in the thoracolumbar and sacral spinal cord of the pig

Evidence for coexistence of choline acetyltransferase (ChAT)- and calcitonin gene-related peptide (CGRP)-immunoreactivity in the thoracolumbar and sacral spinal cord neurons of the pig

Distribution and chemical coding of urinary bladder apex-projecting neurons in aorticorenal and testicular ganglia of the male pig

Enteric nervous system in the European beaver (Castor fiber) pylorus–an immunohistochemical study

Effect of seminal plasma zinc–binding proteins on motility and membrane integrity of canine spermatozoa stored at 5°C

Changes in the expression of galanin and galanin receptors in the wall of the colon in pigs experimentally infected with Brachyspira hyodysenteriae