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Enhanced salt stress tolerance in transgenic potato plants (Solanum tuberosum L.) expressing a bacterial mtlD gene

100%

Rahnama H., Vakilian H., Fahimi H., Ghareyazie B.

Acta Physiologiae Plantarum

2011

tom 33

nr 4

Bacterial mannitol 1-phosphate dehydrogenase (mtlD) gene was introduced into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens-mediated transformation. Transgenic plants were selected on a medium containing 100 mg l⁻¹ kanamycin and confirmed by polymerase chain reaction (PCR), Southern blotting, and RT-PCR analyses. All of the selected transformants accumulated mannitol, a sugar alcohol that is not found in wildtype potato. Experiments designed for testing salt tolerance revealed that there was enhanced NaCl tolerance of the transgenic lines both in vitro and in hydroponic culture. Compared to 0 mM NaCl, the shoot fresh weight of wildtype plants was reduced by 76.5% at 100 mM NaCl under hydroponic conditions. However, under the same condition, the shoot fresh weight of transgenic plants was reduced only by 17.3%, compared to 0 mM NaCl treatment. The improved tolerance of this transgenic line may be attributed to the induction and progressive accumulation of mannitol in the roots and shoots of the plants. In contrast to in vitro experiments, the mannitol content in the transgenic roots and shoots increased at 50 mM NaCl and decreased slightly at 75 and 100 mM NaCl, respectively. Overall, the amount of accumulated mannitol in the transgenic lines was too small to act as an osmolyte; thus, it might act as an osmoprotectant. However, the results demonstrated that mannitol had more contribution to osmotic adjustment in the roots (but not in shoots). Finally, we concluded that mtlD expression in transgenic potato plants can significantly increase the mannitol accumulation that contributes to the enhanced tolerance to NaCl stress. Furthermore, although this enhanced tolerance resulted mainly from an osmoprotectant action, an osmoregulatory effect could not be ruled out.

Detection of genetically modified food in digesta and organs of rats fed transgenic potato

100%

Sattarzadeh A., Rahnama H., Nikmard M., Ghareyazie B.

Journal of Animal and Feed Sciences

2018

tom 27

nr 2

Transgenic potato plants harbouring cry1Ab gene, resistant to potato tuber moth, were used to examine the persistence of recombinant DNA and proteins in the digesta, gastrointestinal tract (GIT) tissues and visceral organs of rats in a 90-day feeding trial. Native plant DNA (chloroplastic gene, cp) and fragments of cry1Ab, nptII and nos promoter were tracked in rat organs and tissues by polymerase chain reaction (PCR). Although complete sequences (1200 bp) of cry1Ab gene and its decreased size (400 bp) were detected in the rat diets, the presence of these sequences was not confirmed by PCR analysis in the GIT contents of rats fed diets containing transgenic potato. Moreover, the 400 bp sequence of the nptII gene and 300 bp of the nos promoter were detected in the caecum, stomach and rectum contents of some rats fed transgenic potato; however, these sequences were not detected in all rats. Immunoassay showed that Cry1Ab protein is detectable in the GIT contents of rats fed transgenic potato, but quantitative assay by ELISA confirmed that the Cry1Ab protein was partially degraded (80.3–83.7% digestibility) after passing through the GIT. Similarly, ELISA assay of NPTII showed that the protein can be digested (76.8–84.36% digestibility) in the GIT. So, although ingested native and recombinant DNA and protein did not totally degrade in the GIT, it can be indicated that there is no need for concern about the effects of recombinant cry1Ab and nptII genes and their products on animals and humans.

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Initiation and origin of stigma-like structures [SLS] on ovary and style explants of saffron in tissue culture

88%

Namin M. H., Ebrahimzadeh H., Ghareyazie B., Radjabian T., Namin H. H.

Acta Biologica Cracoviensia. Series Botanica

2010

tom 52

nr 1

55-60

Saffron, made from the dried stigmas of Crocus sativus L., contains pigments and valuable aromatic compounds, and can be used in medicine and as a spice. Nowadays its production is lower than demand. Tissue culture presents an alternative biochemical tool which can be used to produce stigma-like structure (SLS) in vitro. In this study, the origin and induction of SLS formation was investigated in ovary and style explants of floral buds on MS medium supplemented with 1-naphthalene acetic acid (NAA) and 6-benzlaminopurine (BAP). SLS were directly originated through meristematic cells or indirectly in the form of colorless globular structures from parenchyma tissue. The colorless globular structures initially were conical and pale yellow color at the sharp ends; subsequently they matured into trumpet-like red stigmas with or without finger-like papillae at the margins. Light and electron microscopic observations of ultra- and semithin sections of different developmental stages of SLS showed that these structures possess two kinds of cells: (1) small cells close to parenchyma tissues and (2) large cells oriented towards the peripheral area and apparently originated from the small ones. Our results suggest that the SLS originated from internal parenchyma tissues.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Acta Physiologiae Plantarum

1 Journal of Animal and Feed Sciences

3 Ghareyazie B.

2 Rahnama H.

1 Ebrahimzadeh H.

1 Fahimi H.

1 Namin H. H.

1 Namin M. H.

1 Nikmard M.

1 Radjabian T.

1 Sattarzadeh A.

1 Vakilian H.

1 2018

1 2011

1 2010

Wyniki wyszukiwania

Enhanced salt stress tolerance in transgenic potato plants (Solanum tuberosum L.) expressing a bacterial mtlD gene

Detection of genetically modified food in digesta and organs of rats fed transgenic potato

Initiation and origin of stigma-like structures [SLS] on ovary and style explants of saffron in tissue culture