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Water enters shallow wells from shallow resources of groundwater, therefore the groundwater is especially susceptible to chemical and microbiological pollution. The aim of this study is to determine the effect of intensity of shallow well usage on the nitrate concentration in water. It has been found that concentrations of nitrate ions in the water of shallow wells depends on the quality of water in the basin feeding the well, processes in the water and well depth. Concentration of nitrate ions in newly filled water is higher than that in well water, but due to chemical and biochemical reactions it decreases rather fast and in most cases becomes lower. The rate at which the drained wells refill is different. Re-establishment of the same amount of water ranges between two and five days. In the beginning because of big difference in levels, the rate is higher, and then it slows down and asymptotically approaches the main level. An average rate of filling-up makes 3% of well volume per day, ranging from 7% the first day to 1% the fifth day. The nitrate concentration decreases approximately to eight days at a rate of 8 mg l⁻¹/day, and after the eighth day it increases at a rate of 12 mg l⁻¹. Changes of concentration of nitrates in the top layer of water could be summarized as the reaction of the first order. Mathematical patterns of well refilling and changes in nitrates determine the limits of the nitrate concentration changes in water, though they are not precisely adapted to a separate well.
The rural population, estimated at one third of Lithuania’s population, obtains drinking water from shallow wells. Drinking water from shallow wells is polluted, for the most part, with nitrate, which is of general concern not only in Lithuania but in other countries. The purpose of our study is to establish the relationship of nitrate pollution in the water of shallow wells to precipitation level and temperature, as well as to assess the impact of well construction and environmental conditions on this kind of pollution. The results of this work showed that about 66% of all investigated wells are nitrate polluted. The results of long-term research have demonstrated that the highest nitrate concentration in the water of shallow wells occurs in March through July, and the lowest nitrate concentration in September through February. A strong correlation between nitrate concentration, precipitation amount and air temperature was established in summer. Correlations established in other seasons are not significant. The strongest impact on nitrate pollution of the wells is related to the distances of the well to the outhouse, cowshed, manure pile and vegetable garden, as well as the surroundings of the well and local human activities. The estimated coefficients of multiple linear regression allow forecasting the nitrate pollution of the wells as a function of these factors.
The wild-harvested fruit of Vaccinium oxycoccos (European cranberry) is used medicinally in many European and North American countries; the plant, however, is seldom cultivated. In order to optimize the collection strategy and improve the horticulturally important characters of V. oxycoccos clones, comprehensive investigations of the species are necessary. In the present study we investigated the phenological, morphological and genetic diversity of 29 clones originating from two wild populations growing in two strictly protected Lithuanian reserves, Čepkeliai and Žuvintas. During an ex situ collection at Kaunas Botanical Garden, we observed great phenological variation between the collected V. oxycoccos clones. The following morphological traits most clearly distinguished our study clones: leaf size, berry shape, berry size and fruit colour at full maturity. The genetic variation of V. oxycoccos clones from the two populations was assessed using RAPD and SSR. RAPD analysis conducted with 9 primers resulted in 146 polymorphic loci for the total sample, and SSR analysis with 5 primers revealed 29 alleles for the total sample. A greater degree of polymorphism was demonstrated for the Čepkeliai population than for the Žuvintas population. The study allowed the selection of several clones having promising morphological traits for further testing in the field.
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