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Beta-glucanase productivity improvement via cell immobilization of recombinant Escherichia coli cells in different matrices

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Beshay U., El-Enshasy H., Ismail I. M. K., Moawad H., Abd-El-Ghany S.

Polish Journal of Microbiology

2011

tom 60

nr 2

The studies have been performed to analyze the production of β-glucanase by a recombinant strain of Escherichia coli immobilized in different matrices. Porous sintered glass SIRAN®, Ceramic supporting matrices and Broken Pumice stone as well as SIRAN Raschig-rings were examined for the immobilization of whole bacterial cells. The β-glucanase activity of bacteria immobilized in CeramTec PST 5 (4–5 mm) was very low. CeramTec PST 5 (1.5–2.5 mm) was found to be the best carrier compared to all other matrices regarding glucanase production (630 U/ml) and compared to enzyme activity produced by free cells (500 U/ml). Different doses of matrices were applied (2, 5, 7, 10 g/lask) in the form of “matrix weight”. Using 2 g/flask of CeramTec PST 5 (1.5–2.5 mm) yielded enzyme activity of 630 U/ml). CeramTec gives highest operational stability of β-glucanase by repeated batch fermentation to 5 cycles, and activity reached 660 U/ml. Scanning electron microscopy observations showed a high number of vegetative cells that continued growth inside the matrices, indicating that β-glucanase activity improvement was due to the immobilization of the cells.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Abd-El-Ghany S.

1 Beshay U.

1 El-Enshasy H.

1 Ismail I. M. K.

1 Moawad H.

1 2011

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Beta-glucanase productivity improvement via cell immobilization of recombinant Escherichia coli cells in different matrices