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Feces from wild Triatoma dimidiata induces local inflammation and specific immune response in a murine model

100%

Monteon V., May-Gil I., Nunez-Oreza L., Lopez R.

Annals of Parasitology

2018

tom 64

nr 4

In endemic regions for Triatoma dimidiata the vector for Chagas disease, subjects can be in contact with insect`s feces several times through a lifetime. The triatomine’s digestive tract is colonized by diverse but few dominant genera of microorganisms. The immune responses to microbiota feces are poorly known in mammal hosts. The goal of this paper is to describe the local inflammation at the port of inoculation and the humoral immune response in a murine model mimicking natural contamination of feces from wild Triatoma dimidiata and its identification of bacterial community. Feces from twenty T. dimidiata insects captured in peridomestic and domestic ecotopes were used for bacteria isolation and phenotypic identification. Five microliters of whole feces or bacteria isolated colonies were used for intradermal inoculation of mice for detection of humoral immune response and local inflammation at the inoculation site. The bacterial community identified corresponded to Kytococcus, Brevibacillus, Kocuria, Chryseobacterium, Pantoe, Proteus, Burkholderia, Acinetobacter and Stapylococcus. The local inflammation at the inoculation site was dominated by neutrophils infiltration, and specific seric IgG immune response was recognized against whole feces as well as Burkholderia, Acinetobacter and Staphylococcus isolates. In conclusion, feces from T. dimidiata were colonized by few culturable microorganism genera that are able to induce local inflammation and IgG immune response in a murine model.

Comparative 2-D electrophoresis of salivary proteins in Triatoma dimidiata and Rhodnius prolixus (Hemiptera: Reduviidae) and major cross-reactive antigens

76%

Monteon V., May-Gil I., Acosta-Viana K., Ramos-Logonio A., Hernandez O., Lopez R.

Annals of Parasitology

2017

tom 63

nr 2

An immune response to triatomine’s saliva is an immunological marker of exposure to triatomine bites. However, considerable variability in salivary protein profiles did exist among species. In the present work, we compare salivary proteins from Mexican Triatoma dimidiata and Rhodnius prolixus using 2-D electrophoresis. A clear differential saliva profile was found to exist between these two triatomine species. Fewer protein spots were detected in R. prolixus than in T. dimidiata. More than half of the proteins had an isoelectric point between 5 and 7 and a molecular weight between 10 and 30 kDa in T. dimidiata. Mice exposed to T. dimidiata saliva mount an immune response to three major cross-reacting antigens in R. prolixius saliva with weights of 10 kDa and 55 kDa. Our findings may alert for the presence of cross-reacting antigens between triatomine species in regions where two or more species are overlapping in the same geographical area.

Ograniczanie wyników

2 Annals of Parasitology

2 Lopez R.

2 May-Gil I.

2 Monteon V.

1 Acosta-Viana K.

1 Hernandez O.

1 Nunez-Oreza L.

1 Ramos-Logonio A.

1 2018

1 2017

Wyniki wyszukiwania

Feces from wild Triatoma dimidiata induces local inflammation and specific immune response in a murine model

Comparative 2-D electrophoresis of salivary proteins in Triatoma dimidiata and Rhodnius prolixus (Hemiptera: Reduviidae) and major cross-reactive antigens