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1

Studies on streptokinase. Purification and some molecular properties

100%
Bilinski T., Loch T., Zakrzewski K.
Acta Biochimica Polonica
|
1968
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tom 15
|
nr 1
2

Regulated overexpression of mitochondrial superoxide dismutase (MnSOD) in 10T1/2 cell line

100%
Loch T., Bober E., Braun T.
Acta Biochimica Polonica
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2003
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tom 50
|
nr Supplement 1
3

Studies on streptokinase. The conformation

100%
Loch T., Bilinski T., Zakrzewski K.
Acta Biochimica Polonica
|
1968
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tom 15
|
nr 1
4

Identification of microsatellite region composed of a long homopurine-homopyrimidine tract surrounded by AT-rich sequences upstream of the rat stress-inducible hsp70.1 gene

88%
Lisowska K., Loch T., Fiszer-Kierzkowska A., Scieglinska D., Krawczyk Z.
Acta Biochimica Polonica
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1997
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tom 44
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nr 1
A DNA region containing several repetitive motifs has been detected about 1.9 kbp upstream of the transcription unit of the rat stress-inducible hsp70.1 gene. The most interesting element of this area is a microsatellite sequence (GA)eCAG(TC)24 that consists of an inverted repeat partially overlapping with the long homopurine/homopyrimidine tract (Pu/Py). DNA molecule within the described sequence can theoretically adopt alternate, non-B structures (H-DNA or cruciform) containing single-stranded regions. This microsatellite region is flanked by AT-rich sequences containing several poly(A) tracts. The longest of them with a possible potential to destabilize a double-stranded DNA helix is localized around 160 bp downstream the (GA)6CAG(TC)24. The DNA fragment containing sequences described above was subcloned into the pUCl9 vector and the resulting plasmid was subjected to the standard SI susceptibility assay. Preliminary mapping of the SI cleavage site indicates for the formation of the non-B-DNA structure within the Pu/Py tract. This is to our knowledge a first report on the existence of a complex microsatellite region upstream the 5'-end of the hsp70 gene in mammals.
5

Amplified ribosomal DNA restriction analysis (ARDRA) as a screening method for normal and bulking activated sludge sample differentation

76%
Blaszczyk D., Bednarek I., Machnik G., Sypniewski D., Soltysik D., Loch T., Galka S.
Polish Journal of Environmental Studies
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2011
|
tom 20
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nr 1
In the activated sludge process, the share of filamentous bacteria is crucial for proper settlement and high sewage treatment efficiency. Disequilibrium of the microbial community may be a reason for the appearance of bulking episodes. During 19 months (August 2007-February 2009) activated sludge samples were collected and analyzed by physicochemical, microscopic, and molecular methods. Amplified ribosomal DNA restriction analysis was chosen to detect molecular changes between normal and bulking sludge samples, which may be markers, informing about disadvantageous changes in the microbial population. Quantity and length of restriction products were the basis to create dendrograms representing the phylogenetic relationship of activated sludge samples. The most suitable restriction enzymes for finding molecular differences between normal and bulking sludge were MspI and HhaI.
6

Quantitative RT-PCR assay for mRNA of VEGF and histones H2b, H4 in determination of proliferative and angiogenic activity in vulvar pathology

76%
Michalski B., Mazurek U., Olejek A., Graniczka M., Loch T., Wilczok T., Poreba R.
Folia Histochemica et Cytobiologica. Supplement
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2001
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tom 39
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nr 1
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