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Despite the fact that bone mineral density (BMD) is an important fracture risk predictor in human medicine, studies in equine orthopedic research are still lacking. We hypothesized that BMD correlates with bone failure and fatigue fractures of this bone. Thus, the objectives of this study were to measure the structural and mechanical properties of the proximal phalanx with dual energy X-ray absorptiometry (DXA), to correlate the data obtained from DXA and computer tomography (CT) measurements to those obtained by loading pressure examination and to establish representative region of interest (ROI) for in vitro BMD measurements of the equine proximal phalanx for predicting bone failure force. DXA was used to measure the whole bone BMD and additional three ROI sites in 14 equine proximal phalanges. Following evaluation of the bone density, whole bone, cortical width and area in the mid-diaphyseal plane were measured on CT images. Bones were broken using a manually controlled universal bone crusher to measure bone failure force and reevaluated for the site of fractures on follow-up CT images. Compressive load was applied at a constant displacement rate of 2 mm/min until failure, defined as the first clear drop in the load measurement. The lowest BMD was measured at the trabecular region (mean ± SD: 1.52 ± 0.12 g/cm2; median: 1.48 g/cm2; range: 1.38-1.83 g/cm2). There was a significant positive linear correlation between trabelcular BMD and the breaking strength (P=0.023, r=0.62). The trabecular region of the proximal phalanx appears to be the only significant indicator of failure of strength in vitro. This finding should be reassessed to further reveal the prognostic value of trabecular BMD in an in vivo fracture risk model.
In this study, the effect of a living bacteria (Bacillus megaterium var. phosphaticum, Azotobacter chroococcum) containing biofertilizer, made in Hungary, was investigated on the germination and dry matter production of maize seedlings in germination tests. The biofertilizer was applied in concentrations of 1 ml·L⁻¹ and 3.5 ml·L⁻¹. Seed and filter paper treatments were used in the experiments, completed with autoclaved biofertilizer treatment. Germination and weight of shoots and roots were evaluated. It was observed that the seed-and-filter paper treatments with biofertilizer significantly increased – by more than 20% – the numbers of the germinated seeds in comparison to the untreated control. The dry weight of the shoot and root was higher by more than 7% than the control in the case of treatments with biofertilizer. Based on this result, it was concluded that there is a positive effect of PGPB on germination, as well as it is supposed, that the applied biofertilizer treatments stimulated the germination and growth of maize by reason of excreting phytohormones and enhancing the nutrient mobilization from the seed.
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