Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 4

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Cryopreservation of shoot tips from the endangered endemic species Tuberaria major

100%
Coelho N., Gonzalez-Benito M. E., Romano A.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 12
Tuberaria major is an endangered endemic species from the Algarve, in the south of Portugal. We investigated two techniques for the cryopreservation of T. major shoot tips, namely vitrification and encapsulation-dehydration. Before the cryopreservation trials, shoot tips were precultured for 1 day on liquid Murashige and Skoog (MS) medium containing 0.3 M sucrose. For the vitrification method, shoots tips were exposed for 0, 30, 60, 90 and 120 min to plant vitrification solution 2 (PVS2). As for the encapsulation-dehydration method, shoot tips were dried inside a laminar air flow cabinet for 0, 1, 2, 3, 4, 5 and 6 h at room temperature. The highest regrowth percentages were approximately 60 and 67 % for vitrification and encapsulation-dehydration, respectively. The best times were 60 min exposure to PVS2 for vitrification and 3 h desiccation for encapsulation-dehydration. Though these are preliminary results, the use of the cryopreservation techniques tested here proved to be an important asset in the conservation of this endangered species and will complement the conservation strategies previously developed.
2

Physiological responses of Plantago algarbiensis and P. almogravensis shoots and plantlets to low pH and aluminum stress

76%
Martins N., Osorio M. L., Goncalves S., Osorio J., Palma T., Romano A.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 02
We investigated the impact of low pH and aluminum (Al) stress on the growth, nutrients concentration, chlorophyll a fluorescence, photosynthetic pigment contents, proline and carbohydrate accumulation in shoots and plantlets (leaves and roots) of Plantago almogravensis and P. algarbiensis. Both species accumulated considerable and similar amounts of Al in their tissues, mainly in the roots. The presence of Al caused a significant reduction on root elongation in P. algarbiensis. Low pH and Al induced significant changes on nutrient accumulation, but no significant alterations on the maximum efficiency of PSII (Fv/Fm), quantum yield of PSII photochemistry (ØPSII), quantum yield of regulated energy dissipation (ØNPQ) and quantum yield of non-regulated energy dissipation (ØNO) were detected in both species in response to these stresses. However, Al increased significantly the nonphotochemical quenching and the chlorophyll b content and decreased the PSII excitation pressure (1 - qp) in P. almogravensis leaves. Both stress treatments induced carbohydrate accumulation in the shoots and roots of this species, but not in leaves. In P. algarbiensis, low pH and Al decreased the photosynthetic pigment contents in the shoots, whereas Al stimulated the carbohydrate accumulation in the leaves. Although our data showed that both species are tolerant to Al3+ and H+, P. almogravensis appeared to be more adapted to maintain cellular physiology and growth under those conditions.
3

Morpho-physiological responses of sugar beet (Beta vulgaris L.) genotypes to drought stress

76%
Romano A., Sorgona A., Lupini A., Araniti F., Stevanato P., Cacco G., Abenavoli M. R.
Acta Physiologiae Plantarum
|
2013
|
tom 35
|
nr 03
The identification of morpho-physiological traits related to drought tolerance and high yield potential is a challenge when selecting sugar beet genotypes with greater tolerance to water stress. In this paper, root morphological parameters, antioxidant systems, leaf relative water content (RWC) and H?-ATPase activity as key morpho-physiological traits involved in drought tolerance/ susceptibility of sugar beet were studied. Genotypes showing a different drought tolerance index (DTI) but a similar yield potential, under moderate (-0.6 Mpa) and severe (-1.2 MPa) water stress, were selected and their morpho-physiological traits were investigated. The results showed a wide genetic variation in morpho-physiological parameters which demonstrated the different adaptive strategies under moderate and severe drought conditions in sugar beet. In particular, an efficient antioxidant system and redox signalling made some sugar beet genotypes more tolerant to drought stress. The alternative strategy of other genotypes was the reduction of root tissue density, which produced a less dense root system improving the axial hydraulic conductivity. These results could be considered as interesting challenge for a better understanding of the drought tolerance mechanisms in sugar beet.
4

Cloning two PepT1 cDNA fragments of common carp, Cyprinus carpio [Actinopterygii: Cypriniformes: Cyprinidae]

64%
Ostaszewska T., Szatkowska I., Verri T., Dabrowski K., Romano A., Barca A., Muszynska M., Dybus A., Grochowski P., Kamaszewski M.
Acta Ichthyologica et Piscatoria
|
2009
|
tom 39
|
nr 2
81-86
Background. Common carp, Cyprinus carpio, is a model organism within Teleostei. Oligopeptides are a new and promising alternative source of amino acids in animal as well as in human nutrition. In common carp, the membrane protein that transports oligopeptides across the enterocyte membrane is encoded by the gene PepT1 (SLC15A1). The aim of this paper was to sequence the PepT1 (SLC15A1) in common carp. Materials and Methods. Intestine samples were isolated from six-week old common carp. Total RNA was isolated using a Trizol method. Reverse transcription was used to synthesize cDNA. Two different pairs of primers were designed, according to the zebrafish (Danio rerio) PepT1 sequence, and used for PCR. The amplified DNA was isolated by electrophoresis, cloned (pCRII-TOPO vectors), sequenced, and subjected to in silico analysis. Results. Two nucleotide fragments of the PepT1 gene were obtained and analyzed using bioinformatic tools. Both fragments showed a high degree of homology with the known PepT1 genes of other teleosts, mammals, and birds. High homology of the PepT1 gene, and similar primary protein structure among the aforementioned taxa probably reflects the conservative function of the PepT1 protein product. Both fragments of the PepT1 gene were deposited in GenBank (FJ556590; FJ529670). Conclusion. The sequenced fragments of the common carp PepT1 gene will allow evaluation of PepT1 expression in the intestines of fish fed diets containing various forms of protein, which is an issue of importance regarding fish nutrition and its import for aquaculture.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 1 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.