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The effect on cell viability and content of PhC of three grapes varieties – Moravian Muscat, Blue Burgundy and Lemberger is presented. The effect of polyphenols from wine and grapes was studied for many times, but the effect of pomace, the by-product of wine production, was neglected. Thus study is devoted to compare the effect of berries and pomace on cell viability in context of their utilization as source of bioactive compounds. Effect on viability of human keratinocytes (HaCaT) was investigated in vitro using following concentrations of PhC in cultivation medium: 25, 50, 75 and 100 µg·ml–1. The results show that the content of PhC in berries and pomace was similar and the cell viability decreased with increasing concentrations of PhC, in most cases. The impact on cell viability also depends on individual variety of grapes.
The study aimed at determining whether the combination of three selected genes can be used for selection in small populations of pigs. The estrogen receptor (ESR1), follicle stimulating hormone (FSHB) and porcine myogenin (MYOG) genes were selected to detect their combined effect. Although over the last decades considerable progress in pig production was achieved, reproduction in his species still remains unsatisfactory. The marker-assisted selection (MAS) is considered to bring progress in pig reproduction. If MAS is to be used efficiently, the complexity of reproduction process should be taken into consideration. The effects were studied of interaction between the mentioned three genes and selected production and reproduction traits of pigs. Interactions between the effects of particular gene markers are important because of possible negative effect(s) of a single-path selection as well as possible co-selection. No significant interaction between the effects of genes studied was found. It is concluded that in small populations co-selection is effective only when highly significant effects of selected auxiliary genes occur.
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