The observations were done on calves immunized once oar twice with the „Adenovac 2” in various seasons. The immunogenic properties of the vaccine were evaluated in the SN test on a primary bovine fetal kidney cells. Totally 286 sera of immunized animals collected every 10 days by 30 days from animals immunized once and for 66 days from animals immunized twice were examined. It was found that vaccination induced a high level of neutralizing antibodies (titres from 625 to 2125). A high level of antibodies persisted for 3 weeks after a single and for about 2 months after a double injection of the vaccine. Season does not affect the immune reactivity of the calves.
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