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Genomic DNA library of Veillonella parvula H2 in the rumen

100%

Liu G. W., Zhang Z. G., Yang W. Y., Yang L. Y., Wang Z.

Bulletin of the Veterinary Institute in Puławy

2010

tom 54

nr 1

15-17

To construct a genomic library of Veillonella parvula H2, total DNA was extracted, sheared by a Hydroshear machine, and the DNA fragments ligated a into Smal-digested vector pUC18 before being transformed into E. coli DH5a. Colonies were selected on Luria-Bertani (LB) plates containing ampicillin, 5-bromo-4-chloro-3-indoyl-3-galactose (X-gal), and isopropy-β-D-thiogalactoside (IPTG) and proliferated. Recombinant plasmids were analysed for the presence of inserted DNA fragments of 3-4 kb by restriction mapping. The titre of the library was determined to be 10⁵ pfu/mL according to the formula N=ln(l-p)/(l-f). The genomic library consisted of 99% of the genome of Veillonella parvula, demonstrating a successful library construction.

A note on the distribution of insulin receptor mRNA in tissues of neonatal Holstein calves

86%

Liu G. W., Zhang Z. G., Zhu X. L., Wang Z., Xia C., Xu C.

Journal of Animal and Feed Sciences

2009

tom 18

nr 4

Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method

72%

Zhang Z. G., Liu G. W., Li X. B., Li Y. F., Guo C. M., Gao L., Wang H. B., Xu S. W., Wang Z.

Polish Journal of Veterinary Sciences

2009

tom 12

nr 4

The measurement of D-3-hydroxybutyrate (D-BHBA) in milk samples is an important tool for diagnosis of subclinical/clinical ketosis in dairy cows. We describe a simple UV spectrophotometric method for measuring the concentration of D-BHBA in milk of dairy cows. From two herds, 119 milk samples were taken from dairy cows. The standard-curve equation was y = 0.2582x + 0.0269 (R² = 0.9967). The assay was highly specific with a minimum detection limit of 0.01 mmol/L and measuring range of up to 5 mmol/L. The recovery was between 99.35% and 100.22% and repeatability was 99.8%. The comparison between the spectrophotometric method and the fluorometric method revealed a close correlation (r = 0.9939). These results show that the spectrophotometric method can be successfully used as an alternative method to measure D-BHBA content in milk.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Journal of Animal and Feed Sciences

1 Polish Journal of Veterinary Sciences

3 Liu G. W.

3 Wang Z.

3 Zhang Z. G.

1 Gao L.

1 Guo C. M.

1 Li X. B.

1 Li Y. F.

1 Wang H. B.

1 Xia C.

1 Xu C.

1 Xu S. W.

1 Yang L. Y.

1 Yang W. Y.

1 Zhu X. L.

1 2010

2 2009

Wyniki wyszukiwania

Genomic DNA library of Veillonella parvula H2 in the rumen

A note on the distribution of insulin receptor mRNA in tissues of neonatal Holstein calves

Measurement of milk D-3-hydroxybutyrate with a simple UV spectrophotometer method: an alternative assay method