It was found that pathogens of clamp rot in sugar beet cultivated in Belarus are the following fungal species: Fusarium redolens, Fusarium culmorum, Penicillium expansum, Botrytis cinerea, Gliocladium catenulatum, Sclerotinia sclerotiorum, Alternaria tenuis, Phoma betae. G. catenulatum, F. redolens, F. culmorum, P. expansum, S. sclerotiorum proved more aggressive, P. betae, A. tenuis - less aggressive. Experiments in vitro and in vivo have revealed that strain Bacillus subtilis BIM B-439 D - main component of new biopesticide „Betaprotectin” was characterized by maximal antagonistic activity against clamp rot pathogens. According to small-scale experimental data, „Betaprotectin” biological efficiency reached 36.9-39.2%. Technology of manufacturing new biopreparation comprises a procedure of direct regulation of bacterial antagonistic activity under the impact of physical stress factors - application of spore inoculation material exposed to thermal treatment raised antimicrobial activity of B. subtilis BIM B-439 D liquid culture by 1.2-1.4 times. Deleterious effect of pathogens during storage of root crops in large-scale clamps and piles fell 2-fold upon „Betaprotectin” treatment as compared to the control. Spread of clamp rot slowed down likewise. Biological efficiency of biopesticide application ranged from 17.5% to 39.6%, economic efficiency - from 5.1% to 35.8%. Root crop preservation rate reached 94.1%.
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