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Use of chemical method to control Aedes aegypti population in the Dengue endemic areas caused the emergence of a resistant strain of this species to insecticide compounds, including temephos. Exploration of the alternative compounds that have larvicidal effects is needed, such as natural products derivate from plants. The current study was designed to evaluate the effective dosage of methanolic extract of Manihot glaziovii peel against the third instar larvae of Aedes aegypti. Bioassay test was performed based on the WHO procedure to occupy the 24 hours exposure of five levels dosages of Manihot glaziovii peel extract, namely 1,800, 2,400, 3,000, 3,600 and 4,200 ppm respectively. As many as 25 of healthy Aedes aegypti larvae were subjected into each group, five-time replicated and compared with tap water control group. The mortality rate of Aedes aegypti larvae has increased due to the increase in treatment groups dosages, ranged from 44–97.6%. It is significantly different in larvae mortality rate between different treatment groups. There was no observation of killed larvae in the control group. LD₅₀ and LD₉₀ of methanolic extract of Manihot glaziovii peel were measured 2,027 ppm and 3,772 ppm, respectively. This result suggests the possible use as an ecofriendly larvicide against Aedes aegypti larvae. Further investigation is needed to obtain secondary metabolites of this plant.
Aedes mosquitoes, mainly Aedes aegypti and Aedes albopictus, are the primary and secondary vectors of dengue viruses in Indonesia, with transmission occurring by sucking blood. The density of the vectors is influenced by season and rainfall, but limited by altitude. The aim of the study is to describe the density and distribution of dengue vectors during the dry season based on the altitudes of recent dengue cases in five regencies of Central Java Province, Indonesia. Mosquito larvae and pupae were collected from the indoor and outdoor water containers from 253 houses within 50 m of houses occupied by a dengue patient. A considerable dengue vector population was found in all localities and altitudes based on the Aedes indices: an HI of 41.7% (15.0–70.6), CI of 33.6% (8.1–69.6) and BI of 57.1 (15.0–94.1). The highest indices were found in the highest altitude settlement; as the most common larval habitat in this village was a large-sized cement tank, larvivorous fish can act as effective predators in this case. This finding indicates an expansion of the dengue problem from low to high altitudes, causing a high potential for dengue transmission in all of the localities.
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