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1

Obecność haplotypów mtDNA związanych z "klątwą matki" jako jedna z możliwych przyczyn obniżonego potencjału reprodukcyjnego zająca szaraka w Polsce

100%
Strzala T., Kosowska B., Moska M., Dobosz T.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 03
The aim of the study was to identify carriers of the mtDNA sequence related to the “mother’s curse” in the Polish population of the brown hare. Even slight mtDNA mutations inherited from mothers by their sons may diminish sperm cell motility by decreasing the synthesis of ATP and thus reduce the reproductive success of the species. In the literature this phenomenon is referred to as the “mother’s curse” effect. Muscle samples from 103 hares were collected from hunters in central, southern and eastern Poland. In order to identify hares with the “mother’s curse”, an mtDNA control region (CR) was selected, amplified according to (26), sequenced and analyzed phylogenetically along with sequences from the Genbank, using the PhyML program (9). Four animals were eliminated from mtDNA studies because of heteroplasmy. A tree consisting of 4 clades was generated. For the purpose of this study, the most important of them was the PW clade, which included 5 Polish hares (females) with sequences characteristic of the “mother’s curse”. This constitutes 5.05% of the population studied. The geographical origins of the hares with the “mother’s curse” were dispersed over almost the entire area under investigation. Two hares came from the Płock region, and the others from the Konin, Zamość and Nowy Sącz regions. A small fragment of the mtDNA sequence proved sufficient for the identification of an important functional effect of mutation in the mtDNA on the condition of an individual and the whole population. For the first time a screening method proved effective in the identification of hares with “mother’s curse” mtDNA mutations in a population of animals living in the wild. By then this had only been achieved in captive colonies. The identified group of female carriers, constituting 5.05% of the investigated sample, which persists in the population regardless of selection, may through their sons further compromise the effective size of a constantly decreasing and endangered population of the brown hare in Poland.
2

Analiza filogenetyczna genu 21-hydroksylazy steroidowej u przedstawicieli gromady Mammalia

100%
Strzala T., Frontczak A., Plizga A., Kosowska B.
Zeszyty Naukowe Akademii Rolniczej we Wrocławiu. Zootechnika
|
2004
|
tom 51
311-318
W pracy dokonano analizy filogenetycznej genu 21 -hydroksylazy steroidowej w oparciu o różne typy sekwencji: eksonowe, intronowe oraz aminokwasowe, pochodzące od 7 gatunków gromady Mammalia (człowieka, szczura, myszy, psa, świni, bydła oraz królika). Celem było wytypowanie sekwencji, która na dendrogramie utworzy węzły o wysokiej istotności, spełniając kryteria poprawnej analizy filogenetycznej. Takim typem sekwencji okazały się eksony.
3

Próba amplifikacji sekwencji 21-hydroksylazy steroidowej kilku gatunków ptaków z użyciem zdegenerowanych primerów flankujących gen CYP21 ssaków

100%
Grabowski K., Strzala T., Kosowska B.
Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu. Biologia i Hodowla Zwierząt
|
2011
|
tom 63
4

Wybrane zaburzenia steroidogenezy nadnerczowej ludzi i zwierzat o charakterze dziedzicznym

100%
Brzezinska K., Kosowska B., Moska M., Strzala T.
Acta Scientiarum Polonorum. Medicina Veterinaria
|
2006
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tom 05
|
nr 1
3-9
5

Ssaki Gor Bialskich [Snieznicki Park Krajobrazowy]

100%
Moska M., Bartmanska J., Strzala T.
Parki Narodowe i Rezerwaty Przyrody
|
2007
|
tom 26
|
nr 3
113-124
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Morpholigica similarity and genetic variation of Trochulus striolatus (C. Pffeiffer, 1825) and Trochulus montanus (Studer, 1820) (gastropoda, pulmonata, hygromidae)

100%
Prockow M., Strzala T., Kuznik-Kowalska E.
Folia Malacologica
|
2013
|
tom 21
|
nr 3
7

Charakterystyka genetyczna zajecy [Lepus sp.] w Eurazji

100%
Strzala T., Kosowska B., Brzezinska K., Moska M.
Acta Scientiarum Polonorum. Medicina Veterinaria
|
2006
|
tom 05
|
nr 1
25-31
8

Genetic differentiation between west and east european karyotypic groups of Sorex araneus (Soricomorpha) in Poland

88%
Moska M., Wierzbicki H., Strzala T., Mucha A., Jonkisz A.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2014
|
tom 17
|
nr 2
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Multi-method approach to identification of species of Trochulus (Gastropoda: Hygromiidae)

88%
Prockow M., Strzala T., Kuznik-Kowalska E., Prockow J., Mackiewicz P.
Folia Malacologica
|
2016
|
tom 24
|
nr 1
10

Molecular phylogenetic analysis of the domestic cat and the Eurasian lynx based on Cyp21gene

76%
Kosowska B., Strzala T., Brzezinska K., Grabowski K., Dobosz T., Lebioda A.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 03
The aim of the study was to determine the nucleotide sequence of the CYP21 gene of the Eurasian lynx, eight representatives of five selected domestic cat breeds and a European mixed-breed cat on account of the key role of this gene product in adrenal steroidogenesis. Cats nuclear DNA was obtained from peripheral blood, while the lynx DNA was isolated from muscle tissue. 21HS gene amplification was performed in 10 parts with the unified PCR conditions. The reaction products were sequenced. On the basis of the determined nucleotide (nc) sequence of the gene, the sequence of amino acids (aa) of the protein was determined. The sequences obtained in our study and collected from the GeneBank were aligned using Mafft and subjected to phylogenetic analysis using the program MrBayes 3.2. A total of 11 SNP’s were detected in 9 tested cat breeds and an additional 3 indels in the mixed-breed cat. Between the studied cats and lynx a total of 33 SNP differences were determined. In addition, three indels were located in the lynx that were absent in cats. In domestic cats the 21HS gene occurs as a series of three long alleles: 2500 bp, 2503 bp, and 2504 bp. Both alleles of lynx were 2502 bp in length. Genetic distance was identified between the studied breeds of cats, as well as between cats and the lynx. A genetic analysis of Cyp21 nucleotide sequences of cats and lynx was performed against homologous sequences obtained from GeneBank, derived from 13 different vertebrate species. In addition, evolutionary distance was estimated between the amino acid sequences (aa) of the cat/lynx and homologous sequences obtained from the GeneBank and derived from 15 different vertebrate species. It was demonstrated that the aa sequence of cat is almost identical to the sequence of lynx. Overall, basing on 21HS aa sequence, the closest kinship links felines and canids, then felines with pigs and ruminants, after which were rodents. The lowest CYP21 kinship links aa sequences of felines with eels and birds, followed by humans and apes.
11

Gene polymorphisms of novel immunotolerant molecule BTLA: distribution of alleles, genotypes and haplotypes in Polish Caucasian population

76%
Partyka A., Woszczyk D., Strzala T., Szczepanska A., Tomkiewicz A., Frydecka I., Karabon L.
Archivum Immunologiae et Therapiae Experimentalis
|
2015
|
tom 63
|
nr 1
12

A microsatellite study in the Łęgucki Młyn/Popielno hybrid zone reveals no genetic differentiation between two chromosome races of the common shrew (Sorex araneus)

76%
Moska M., Wierzbicki H., Macierzynska A., Strzala T., Maslak R., Warchalowski M.
Acta Theriologica
|
2011
|
tom 56
|
nr 2
This study investigated a chromosome hybrid zone between two chromosomal races of the common shrew (Sorex araneus). Gene flow and genetic structure of the hybrid zone, located in the northeast of Poland, were studied using seven polymorphic autosomal microsatellite loci (L9, L14, L33, L45, L67, L68, L97) and a Y-linked microsatellite locus (L8Y). Seventy-five animals (46 of the Łęgucki Młyn race and 29 of the Popielno race) from nine different localities were examined and the data were analyzed using hierarchical AMOVA and F-statistic. The studied microsatellite loci and races (divided into nine geographical populations) were characterized by observed heterozygosity (H O), expected heterozygosities within (H S), and between (H T) populations, inbreeding coefficient (F IS), fixation index (F ST), and average allelic richness (A). We found that genetic structuring within and between the two chromosome races were weak and non-significant. This finding and unconstrained gene flow between the races indicates a high level of migration within the Łęgucki Młyn/Popielno hybrid zone, suggesting that evolutionarily important genetic structuring does not occur in interracial zones where races which are not genetically distinct come into contact.
13

Parametry zmienności genetycznej loci DRB1 i DQA1 MHC klasy II zajęcy polskich (Lepus europaeus)

76%
Kosowska B., Strzala T., Dziedzic B., Majka L., Grabowski K., Moska M., Dobosz T.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 03
The aim of the study was to evaluate the parameters of genetic variability at the MHC class II DRB1 and DQA1 loci in 112 brown hares from 4 regions of Poland. Disturbances in the Hardy-Weinberg (HW) equilibrium associated with an increase in homozygocity at these loci may lead to an increase in the proportion of females whose fetuses have compatible MHC haplotypes. This compatibility may result in abortion, which diminishes the reproductive success of the species. The extraction of nuclear DNA from peripheral blood Iymphocytes was performed with GenElute Blood Genomic DNA Kit manufactured by Sigma-Aldrich. DNA isolation from muscles was performed with Sherlock AX set manufactured by A&A Biotechnology. Primers and PCR conditions for the second DQA gene exon were conducted according to (34). Alleles obtained were analyzed for polymorphism by the SSCP method with modifications according to (34). The following primers were used to obtain exon 2 of the DRB gene: DRB2F 5’ - GAG TGT CAT TTC TAC AAC GGG A - 3 `, DRB2R 5’ - CTC CCG AAC CCC GTA GTT GTG TTT GC - 3’. Other reaction conditions were the same as for the DQ gene. Alleles obtained were analyzed with the Arlequin 3.5 program (20). The following parameters were analyzed: expected and observed heterozygocity, the inbreeding level (FIS), and the fixation index (FST). The number of alleles in the population, the number of private alleles, the number of homozygotes and heterozygotes, as well as the allelic richness (R) were determined with the FSTAT v.2.9.3.2 program (23). FIS for the DRB1 locus revealed an HW disequilibrium with a significant excess of DRB1 homozygotes, especially in the Kalisz region. FIS for the DQA 1 locus revealed an HW equilibrium in the Kalisz and Oświęcim regions, whereas in the other regions (Ciechanów and Płock) there was a slight excess of heterozygotes. These results are the first findings for the MHC class II DRB1 and DQA1 loci in a population of Polish hares living in the wild. The above picture of changes in the frequency of genes and DRB1 and DQA1 genotypes shows tendencies towards decreasing heterozygocity and increasing homozygocity. This reveals the local incidence of highly unfavorable phenomena associated with an inadequate gene exchange, resulting in a disturbed HW equilibrium. These findings suggest that poor reproductive performance of Polish hares may be indirectly related to a disturbed HW equilibrium and a significant increase of homozygocity at the MHC class II DRB1 locus.
14

Mutacje eksonu 1, 11 oraz 20 genu TCOF1 u chorych z zespołem Treachera Collinsa

76%
Wierzbicka B., Marszalek-Kruk B., Wojcicki P., Smigiel R., Kosowska B., Moska M., Strzala T.
Zeszyty Naukowe Uniwersytetu Przyrodniczego we Wrocławiu. Biologia i Hodowla Zwierząt
|
2009
|
tom 59
15

Genetic variability of the Polish brown hare (Lepus europaeus) based on PCR-RFLP mtDNA analysis (preliminary results)

76%
Strzala T., Stamatis C., Kosowska B., Moska M., Marszalek-Kruk B., Mamuris Z.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2008
|
tom 11
|
nr 1
16

Phylogenetic analysis of steroid 21-hydroxylase (CYP21) of the wolf and selected breeds of dogs in connection with the role of this enzyme in the pathogenesis of many diseases in the Canidae family

76%
Kosowska B., Strzala T., Brzezinska K., Grabowski K., Moska M., Dobosz T., Lebioda A.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 09
The aim of this study was to determine the nucleotide sequence of the CYP21 gene in the wolf and representatives of five breeds of dogs (selected according to a classification by Parker et al, 2004) in connection with the key role of the product of this gene in the genesis of many diseases in dogs. Nuclear DNA of dogs was obtained from peripheral blood, and the wolf’s DNA was isolated from muscle tissue. The amplification of the 21HS gene was carried out in 10 fragments under standardized PCR conditions. The reaction products were sequenced. The sequence of amino acids in the protein was determined on the basis of the nucleotide sequence. The sequences obtained in our study and those retrieved from the GeneBank database were compared with the Mafft program (15) and subjected to phylogenetic analysis with the MrBayes 3.2 program (35). We detected a total of nine SNP mutations in introns and exons. Furthermore, a deletion of two nucleotides, that differentiates the breeds, was detected in the promoter region. Only two differences between the dogs and the wolf were found in SNP: one in an exon and one in an intron. Genetic distance was determined between the selected breeds of dogs and between the wolf and the dogs of each breed. In addition, we estimated the evolutionary distances between amino acid sequences of the dog/wolf and homologous 21HS sequences of eight different vertebrate species obtained from GeneBank. It was shown that, among mammals, the amino acid sequence of the dog/wolf is the most similar to the sequence of the pig, and the least similar to that of the human. The sequences determined in this study may provide a reference point for the research on the CYP21 gene structure and expression in various tissues of dogs for therapeutic purposes.
17

Life-span of long and rough coated dachshunds in dependence of their inbreed level and sex

64%
Kosowska B., Strzala T., Moska M., Kujalowicz J., Oryszczak E., Piecyk J., Sawczuk M., Slusarczyk J., Wasilewska K.
Electronic Journal of Polish Agricultural Universities. Series Veterinary Medicine
|
2005
|
tom 08
|
nr 4
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