Wyniki wyszukiwania

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Anthocyanin accumulation and related gene expression affected by low temperature during strawberry coloration

100%

Zhang Y., Liu Y., Hu W., Sun B., Chen Q., Tang H.

Acta Physiologiae Plantarum

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2018

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tom 40

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nr 11

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Effects of soil temperature on bud break, shoot and needle growth, and frost hardiness in Pinus sylvestris var. mongolica saplings during dehardening

100%

Zhang G., Sun B., Li Z., Di B., Xiang C., Meng Y.

Acta Physiologiae Plantarum

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2017

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tom 39

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nr 08

3

Molecular cloning and functional characterization of a glyceraldehyde-3-phosphate dehydrogenase gene from Spartina alterniflora reveals its involvement in salt stress response

100%

Yuan H., Cai L., Wang P., Sun B., Xu S., Xia B., Wang R.

Acta Physiologiae Plantarum

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2019

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tom 41

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nr 07

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been emerging to be a multifunctional protein involved in various cellular processes, in addition to its role in energy metabolism. In this study, the SaGAPDH gene was cloned from Spartina alterniflora based on the full-length cDNA library. The open reading frame of SaGAPDH was 1014 bp, and it was encoding 337 amino acids with a calculated molecular mass of 36.40 kDa. Multiple sequence alignment showed that SaGAPDH had high amino acid sequence identity with other plant GAPDHs, and phylogenetic analysis demonstrated that SaGAPDH had a closer affinity to GAPDH in Aeluropus lagopoides (AlGAPDH). Subcellular localization suggested that SaGAPDH was located in cytosol. The recombinant SaGAPDH protein was expressed in Escherichia coli cells to characterize its catalytic activity. And E. coli carrying SaGAPDH gene showed an increased salt stress resistance. SaGAPDH gene was induced by salt stress, and to further investigate its function, transgenic Arabidopsis plants ectopically antisense-overexpressing SaGAPDH was generated. The transgenic Arabidopsis plants showed a specific down-regulation of AtGAPC1 transcript and the GAPDH enzyme activity. They also showed decreased tolerance to salt stress and down-regulation of antioxidant enzymes including catalase, ascorbate peroxidase, superoxide dismutase, and peroxidase, as well as their transcripts. Above results were further confirmed by the aggravation of oxidative damage in SaGAPDH antisense-overexpressing transgenic Arabidopsis lines, which accumulated more reactive oxygen species (ROS) such as superoxide anion (O₂˙⁻) and hydrogen peroxide (H₂O₂) under salt stress. This study indicated that SaGAPDH may play an important role in response to salt stress by the regulation of redox homeostasis.

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Composition of ethyl acetate extracts from three plant materials (shaddock peel, pomegranate peel, pomegranate seed) and their algicidal activities

100%

Wang H., Kang H., Zhang L., Cheng S., Liu H., Liu H., Sun B.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 4

Many studies have involved the isolation and identification of allelochemicals from aquatic plants, but the algicidal properties of terrestrial plants have received less attention. This study aims to identify allelochemicals of ethyl acetate extracts from three plant materials (shaddock peel, pomegranate peel, pomegranate seed) and to investigate their inhibitory effects on Microcystis aeruginosa. The ethyl acetate extracts of the three plant materials were identified by GC-MS. Finally, 19 kinds of compounds (including organic acids, ester, ketone, sterol, etc.) were obtained and eight kinds of organic acids and N-phenyl-2-Naphthalenamine were proved to be allelochemicals. The inhibitory effects of the ethyl acetate extracts were also explored by M. aeruginosa bioassay. This showed that the inhibition percentages of ethyl acetate extracts of the three plant materials on the growth of M. aeruginosa were 43.9%, 47.5%, and 40.3%, respectively, when the algae were treated at a dosage of 20 mg/L extracts.

5

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Abundance and community structure of ammonia oxidizing archaea and bacteria in response to peanut growth under controlled condition in Shandong, China

100%

Huang C., Xu N., Fu Z., Sun B., You S., Ma D., Yu J.

Acta Scientiarum Polonorum. Hortorum Cultus

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2019

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tom 18

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nr 6

Based on a three-year field experiment under controlled condition in Ji’nan, China, the effects of peanut growth on the variation in the abundance and community structure of ammonia oxidizing bacteria (AOB) and archaea (AOA) before and after peanut growth were investigated through quantitative PCR and cluster analysis of terminal-restriction fragment length polymorphism. Our results show that the community composition of AOA and AOB was greatly affected by the peanut growth leading to the decreased abundance of AOA and increased abundance of AOB. Furthermore, AOA and AOB community structures varied before and after peanut growth. Phylogenetic analysis indicated that all AOA and AOB community sequences were clustered into the uncultured group. Altogether, the results suggested that the abundance of AOA and AOB in soil and their community compositions can be greatly affected by the peanut growth.

Ograniczanie wyników

Anthocyanin accumulation and related gene expression affected by low temperature during strawberry coloration

Effects of soil temperature on bud break, shoot and needle growth, and frost hardiness in Pinus sylvestris var. mongolica saplings during dehardening

Molecular cloning and functional characterization of a glyceraldehyde-3-phosphate dehydrogenase gene from Spartina alterniflora reveals its involvement in salt stress response

Composition of ethyl acetate extracts from three plant materials (shaddock peel, pomegranate peel, pomegranate seed) and their algicidal activities

Abundance and community structure of ammonia oxidizing archaea and bacteria in response to peanut growth under controlled condition in Shandong, China