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1

Partial purification and characterization of pectinmethylesterase from ripening guava (Psidium guajava L.) fruits

100%
Mondal K., Malhotra S. P., Jain V., Singh R.
Acta Physiologiae Plantarum
|
2009
|
tom 31
|
nr 1
Employing the techniques of (NH₄)₂SO₄ fractionation, ion exchange chromatography on DEAE cellulose and gel filtration through Sephadex G-100, pectinmethylesterase (EC 3.1.1.11) was purified from guava (Psidium guajava L.) fruits var. Hisar Safeda harvested at turning stage of maturity to 129-fold with 28% recovery. Molecular weight as determined by gel filtration was found to be 51 kDa and the enzyme preparation exhibited the same molecular weight under native (Native-PAGE) and denaturating conditions (SDS-PAGE) indicating that the enzyme was a monomer. With pectin as the substrate, it exhibited the Michaelis Menten kinetics with Km value of 3.1 g l⁻¹. The enzyme was found to be stimulated by Ca⁺⁺ and Na⁺ and inhibited competitively by Dgalacturonic acid with Ki value of 1.97 mM. The enzyme was completely inactivated by iodine while with diethyl pyrocarbonate and N-acetylimidazole, the enzyme was inhibited up to the extent of 56 and 45%, respectively. However, DTNB had no inhibitory effect whatsoever precluding the participation of any –SH group in the active centre. It is tentatively proposed that the enzyme has tyrosine and histidine residues at its active centre.
2

Status and diversity of avifauna in coastal areas of South Bengal, India

100%
Payra A., Mondal K., Mishra R. K., Rout S.
World Scientific News
|
2017
|
tom 74
3

Factors infuencing the distribution of leopard in a semiarid landscape of Western India

100%
Mondal K., Sankar K., Qureshi Q.
Acta Theriologica
|
2013
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tom 58
|
nr 2
Previously, factors governing distribution of leopard (Panthera pardus fusca) in forest habitats of the Indian subcontinent were unknown. The present study assessed the influence of different ecogeographic variables determining the distribution of leopards in and around Sariska Tiger Reserve through MaxEnt habitat suitability model based on camera trapping method. Camera trapping was used to collect presence/absence information in the study area from December 2008 to June 2010. Information of 11 macrohabitat characteristics and variables (habitat types, prey species, Normalized Difference Vegetation Index (NDVI), elevation, livestock, village, water source, etc.) were collected along with leopard presence data. The probability of presence of leopards increased with decreasing distance to water and increasing encounter rate of peafowl (Pavo cristatus), chital (Axis axis), sambar (Rusa unicolor), and wild pig (Sus scrofa). It was found that the probability of presence of leopards increased with increasing area of Zizyphus mixed forest patches and NDVI. Results of this study showed that the probability of presence of leopards was higher in habitat types with intermediate cover, high wild prey base, and water sources. They also indicated that leopards are not always ‘generalists’ showing some degree of specialization, at least in their choice of habitat, and this information is useful for conserving leopard in human-dominated landscapes.
4

Evaluation of fermented fish offal in formulated diet of the Indian major carp, rohu, Labeo rohita [Hamilton]

88%
Mondal K., Kaviraj A., Mukhopadhyay P. K., Datta M., Sengupta C.
Acta Ichthyologica et Piscatoria
|
2007
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tom 37
|
nr 2
99-105
Background. Carp fish offal contains high amount of protein and lipid. The quest for protein sources alternative to fish meal has been a priority in aquaculture research because of growing concerns on the future availability of fish meal for incorporation in fish diets. This study determined the extent to which carp fish offal could be fermented and used to replace fish meal in the formulation of diet for fingerlings of Labeo rohita. Materials and Methods. Fish offal, comprising viscera of carp, was fermented along with mustard oil cake and rice bran anaerobically by a microbial suspension (108 cells · mL-1) under ambient temperature (27-30°C). The fermented mixture was mixed with fish meal, mineral- and vitamin mixture to prepare three experimental diets: 1) a reference diet (T1), without any fermentation mixture, containing 40% fish meal, 2) a diet containing 25% fish offal (fermented) and 25% fish meal (T2), and 3) a diet containing 30% fish offal (fermented) and 20% fishmeal (T3). These diets were tested to evaluate growth and biochemical composition of the body of the fingerlings of Labeo rohita and apparent protein digestibility (APD) and intake rate of the diets by the fish. Proximate analysis of feed ingredients, experimental diets, faecal samples and the carcass were performed following the AOAC procedures. Results. Fingerlings of Labeo rohita, reared for 60 days with the diets containing fermented fish offal mixture (T2 and T3), showed higher growth and higher values of protein and lipid in the carcass than those reared with the reference diet (T1). Apparent protein digestibility of the diets did not vary significantly, but average feed intake rate of the T2 and T3 diets were higher than the T1 diet. Conclusion. Fermented fish offal is a viable alternative of fish meal in the formulation of diet for fingerlings of Labeo rohita. Fifty percent replacement of fish meal is possible by a mixture containing 30%fish offal. Further studies are required to improve quality of the fermentation mixture and increase the rate of replacement of fish meal.
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