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Stimulatory and period-specific effect of nitric oxide on in vitro caulogenesis in Albizzia lebbeck (L.) Benth.

100%

Karla C., Babbar S. B.

Acta Physiologiae Plantarum

2012

tom 34

nr 1

The paper reports stimulatory effect of nitric oxide (NO) on in vitro caulogenesis in Albizzia lebbeck, a tree legume. Exogenously supplied NO donor, sodium nitroprusside (SNP) stimulated shoot differentiation from hypocotyl explants of Albizzia lebbeck, excised from its in vitro seedlings. Potassium ferrocyanide, a structural analog of SNP incapable of releasing NO, did not promote shoot organogenesis. Likewise, metabolic products of NO, NO₂- and NO₃ -, provided as NaNO₂ and NaNO₃ did not enhance shoot differentiation. The NO scavenger, 2-(4-carboxy-phenyl)-4, 4, 5, 5-tetramethylimideazoline-1-oxyl-3-oxide (cPTIO), supplemented along with SNP, at equimolar concentration, reversed the stimulatory effect of the latter, thus, confirming the role of NO in promotion of in vitro caulogenesis. The transfer of explants cultured on the basal medium (BM) to the same containing SNP and vice versa after different time intervals revealed that for its enhancing effect, SNP was required only during the initial phase (5 days) of culture. Its presence or administration beyond 5 days neither promoted nor inhibited the caulogenic response.

Nitric oxide is involved in salicylic acid-induced flowering of Lemna aequinoctialis Welw.

80%

Khurana A., Kumar R., Babbar S. B.

Acta Physiologiae Plantarum

2014

tom 36

nr 10

Salicylic acid (SA) is a well-known inducer of flowering in Lemna under both non-inductive and inductive photoperiod conditions. However, the underlying mechanism is not well understood. Herein, we report for the first time that nitric oxide (NO) is partially involved in SAinduced flowering in L. aequinoctialis (Syn. L. paucicostata Hegelm.). Our results demonstrated that SA-induced flowering is significantly reduced by exogenous application of NO scavengers; 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide and methylene blue, nitric oxide synthase inhibitors; N-ω-nitro-L-arginine and N-ω-nitro-L-arginine-methyl ester hydrochloride, and nitrate reductase inhibitor; sodium tungstate in two strains of Lemna viz. 6746 and LP₆. Altogether our present findings shed a light on the new role of NO in SA-induced flowering and open interesting directions that need further investigation.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Babbar S. B.

1 Karla C.

1 Khurana A.

1 Kumar R.

1 2014

1 2012

Wyniki wyszukiwania

Stimulatory and period-specific effect of nitric oxide on in vitro caulogenesis in Albizzia lebbeck (L.) Benth.

Nitric oxide is involved in salicylic acid-induced flowering of Lemna aequinoctialis Welw.