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Biochemical and molecular analysis of superoxide dismutase in sordaria fimicola and Aspergillus niger collected from different environments

100%
Ishfaq M., Mahmood N., Nasir I. A., Saleem M.
Polish Journal of Environmental Studies
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2017
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tom 26
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nr 1
We evaluated Sordaria fimicola strains collected from benign and harsh environments of Evolution Canyon 1 (EC 1) for superoxide dismutase (SOD) enzyme activity, and analyzed their respective gene sequences, which were then submitted to the NCBI database for the first time. Ten strains of Aspergillus niger were used as control in a SOD assay. In enzymatic analysis, among 61 isolates the N6 strain of S. fimicola was found to be the most efficient as it caused 50% inhibition of NBT (Nitro-blue tetrazolium) reduction at 20 µg of the SOD protein, while in A. niger, strain 744 showed 60% inhibition of the NBT reduction at 40 µg amount of SOD protein and was found to be most efficient among A. niger. The superoxide dismutase-1 (SOD-1) gene (including exones and introns; 960 bases) was amplified and sequenced from biochemically efficient strains of S. fimicola viz. N6, N7, S2, S1, and SF13, and submitted to the NCBI database under accession numbers KM282180, KM282181, KM282179, KM282178, and KM282177, respectively. On comparison with the reported sequence of Neurospora crassa (M58687.1), a total of 25 base substitutions and seven amino acids changes were detected in the in silico translated proteins of all five strains of S. fimicola compared to the reference sequence of N. crassa (M18334.1). The biochemical as well as molecular data of the study proved that environmental stresses affected the SOD-1 gene by bringing in mutations, which may result in genomic diversity among their frontier molecules such as proteins. The observed enzymatic activity of SOD in S. fimicola strains was reported to be even better and was comparable to A. niger strains, and thus S. fimicola strains can be exploited further for enzymatic production for industrial use.
2
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A lag in the advancement of biotechnology; reliable control of maize stem borers in Africa

76%
Samuel A. O., Tabbassum B., Sharif M. N., Bhatti M. U., Nasir I. A., Husnain T.
Journal of Plant Protection Research
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2018
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tom 58
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nr 1
The future of food security in Africa is being severely threatened due to an exponential increase in population, which is almost three times the increase of food production. Maize production is constrained by stem borers which cause significant yield losses. Yield losses can be further compounded by higher temperatures due to climate changes, which are expected to increase the population of maize stem borers. While several methods have been employed in stem borer management, there is still significant damage caused by maize stem borers. This necessitates better control methods including the adoption of recent biotechnological advancement in RNA interference (RNAi) technology. This review highlights evidence of an increase in the stem borer population as well as the foreseen decline in maize production worldwide due to the effects of climatic changes. Furthermore, we have drawn attention to improved methods that have been used to control stem borers in maize production as well as a reluctant acceptance of traditional biotechnology in Africa. Finally, we suggest the application of alternative RNA interference techniques to breed maize for efficient pest control in order to achieve food security, improve nutrition and promote sustainable maize production.
3
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Potential of endochitinase gene to control Fusarium wilt and early blight disease in transgenic potato lines

64%
Fatima N., Tabassum B., Yousaf I., Malik M., Khan A., Sajid I. A., Tariq M., Toufiq N., Riaz S., Nasir I. A.
Journal of Plant Protection Research
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2019
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tom 59
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nr 3
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