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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Variation in the activity of stearoyl-CoA desaturase enzyme and endogenous precursors of unsaturated fatty acids in cow's milk during grazing season

100%
Kuczynska B., Puppel K., Nalecz-Tarwacka T., Czub M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
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2015
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tom 54[1]
Variation in the activity of stearoyl-CoA desaturase enzyme and endogenous precursors of unsaturated fatty acids in cow's milk during grazing season. The aim of this study was to determine variation in the activity of stearoyl-CoA desaturase enzyme and endogenous precursors of unsaturated fatty acids in cow's milk during the grazing season. The study was carried out in 10 certified organic farms The major criterions of choosing organic farms to the experiment were as follows: obtaining certificate for at least 5 years and abidance of the organic farming standards, possessing a herd of Black and White Polish Holstein-Friesian cows (numbering more than 30 cow. The highest concentrations of CLA cis9, trans11 isomer, TVA and LA in milk fat has been reported in June, slightly lower in July and August. While the lowest level has been recorded in May, at the beginning of grazing period. Significant correlations between MUFA and SCD (r2= 0.828 p<0.01) has been recorded. We concluded, that monitoring of SCD activity can be used as a tool to achieve high unsaturation of milk fat, during grazing period.
2
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Changes in the concentration of selected minerals in milk and blood of high-milking cows during lactation

64%
Nalecz-Tarwacka T., Golebiewski M., Kuczynska B., Puppel K., Wojcik A., Brzezinska M., Czub M., Benet M.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
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2015
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tom 54[1]
Changes in the concentration of selected minerals in milk and blood of high- milking cows during lactation. The aim of this research was the analysis of the content of calcium, phosphorus and magnesium in milk and blood of Polish Holstein-Frisian (PHF) milking cows during full lactation. The research was conducted on 100 PHF cows during two years (50 cows in each year). Samples of milk and blood were taken in each month of lactation (10 samples) in order to determine minerals concentration. Additionally, samples of forage were pooled out. Study revealed that the level of calcium in the feed is too low, the level of phosphorus – optimal, whereas the content of magnesium exceeds animal demand. There were significant differences in the content of calcium, phosphorus and magnesium in the consecutive months of lactation. The content of minerals in blood was normal; similar situation was observed in the case of the calcium and phosphorus in milk, with the exception of milk magnesium concentration, which was too low and most diversified during the lactation.
3
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Relationships between milk beta-carotene concentrations and the cytological quality of cow's milk

64%
Kuczynska B., Kapusta A., Puppel K., Nalecz-Tarwacka T., Budzinski A., Golebiewski M., Czub M., Grodzki H.
Annals of Warsaw University of Life Sciences - SGGW. Animal Science
|
2015
|
tom 54[1]
Relationships between milk β-carotene concentrations and the cytological quality of cow’s milk. The objective of the study was to describe the relationship between milk β-carotene concentration and the cytological quality of milk of high yielding cows. The experiment was carried out at the research dairy farm of the Warsaw University of Life Sciences (WULS). From a herd of 320 Polish Holstein Friesian cows maintained in a free–stall dairy shed 58 cows were selected taking into consideration the stage of lactation (15±14 days). Cows were fed a total mixed ration (TMR) diet provided ad libitum. Four groups of cows taking into consideration concentration of β–carotene in milk has been created: 1) <150 mg L-1; 2) 151-250 mg L-1; 3) 251-450 mg L-1; 4) > 450 mg L-1. The content of β–carotene and SCC amounted at the herd level: 192 tys ml-1 and 0.312 mgL -1 respectively. The β–carotene content ranged from 0.150 to 0.451 mgL-1. The highest content of β–carotene, 0.451 mgL-1, was found in milk of cows with the lowest level of somatic cells count (SCC). The lowest level, 0.150 mgL-1, was found in milk of cows with the highest level of SCC. Milk SCC varies significantly with the concentration of β-carotene in cow’s milk. Researchers should consider monitoring for this variable as potential cofounder when exploring the relationship between mastitis, intramammary infection and nutritional management.
4

Ocena kondycji i stanu zdrowotnego krów mlecznych w okresie zasuszenia i pierwszej fazy laktacji

64%
Kuczyńska B., Golebiewski M., Nalecz-Tarwacka T., Puppel K., Chmielewski L., Wojcik A., Budzinski A., Kapusta A., Czub M.
Przegląd Hodowlany
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2014
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tom 82
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nr 3
The aim of this study was to determine the relationship between the body condition score of dairy cows and their performance and health status, expressed as the chemical composition of milk and blood biochemical indices. The body condition of 53 PHF cows was evaluated during the dry period and the first 100 days of lactation. In early lactation (100 days) samples of milk and blood were collected as well. In three consecutive measurements during the dry period (60, 30 and 10 days before calving), cows with body condition scores >4 BCS points constituted the largest group in the study population (58.49%, 45.28% and 58.33%, respectively). The greatest decline in body condition during the study period occurred in cows whose body condition was highest during the dry period (>4). The highest average milk yield was obtained in cows whose body condition score in the dry period ranged from 3.1 to 3.5. Significant differences were noted in the content of the milk components (protein, lactose, casein, non-fat dry matter) and in metabolic profile indicators (protein, creatinine, AST) between cows with different body condition scores in the dry period and the first 100 days of lactation.
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Relationship between DNA replication and DNA repair in human lymphocytes proliferating in vitro in the presence and in absence of mutagen

64%
Szyfter K., Wiktorowicz K., Wielgosz M. S., Zajaczek S., Kujawski M., Jaloszynski P., Czub M., Markowska J.
Journal of Applied Genetics
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1995
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tom 36
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nr 4
The effects of mutagens on DNA replication and DNA repair were studied in peripheral blood lymphocytes (PBL) obtained from 21 healthy subjects, 2 samples from healthy heterozygote of Xeroderma pigmentosum (XP) and 2 samples from patient with clinically recognised XP. Inter-individual variations were found in DNA replication and in the level of spontaneous DNA repair measured under standard culture condition. Exposure of human PBL proliferating in vitro to B(a)P was followed by a partial inhibition of replicative DNA synthesis in all subjects and by an induction of DNA repair in healthy subjects. In XP patients DNA repair synthesis remained at the level attributed to spontaneous DNA repair. The response to mutagen varied individually. Results were analysed statistically. It was established that the studied indices of DNA synthesis correlate well with each other. The highest correlation was found between the levels of spontaneous and B(a)P-induced DNA repair. It is concluded that the level of spontaneous DNA repair is predictive for an estimation of cells ability to repair DNA damage. Inter-individual variations in the inhibition of DNA replication and in DNA repair synthesis are also dependent on the type of mutagen as shown by effects of other mutagens. Different effects of mutagen exposure on the inhibition of DNA replicative synthesis and induction of DNA repair can be explained by genetically controlled differences in the activity of enzymes responsible for mutagen processing and lesion removal.
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