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Improving an in vitro propagation protocol for Cestrum nocturnum L.

100%

Rasheed K. A.

Acta Agrobotanica

2013

tom 66

nr 2

The present study was carried out to assess the micropropagation of Cestrum (Cestrum nocturnum L.) by using single nodes and shoot tips excised from soft cuttings using MS salts, 30 g × l-1 sucrose, 7 g × l-1 agar, and different concentrations of plant growth regulators in culture medium. The results revealed that the use of mercuric chloride (0.05%, HgCl2) for 7 minutes was very effective in preventing contamination and gave the highest survival percentage (99%). The highest response (100%) was gained at initiation stage from lateral bud explants on MS medium supplemented with 1.5 mg × l-1 of BA with most of NAA concentrations. However, in case of terminal buds, higher percentages of responses were resulted from the interaction of BA (1.5 mg × l-1) with 0.2 mg × l-1 NAA. The lateral buds also produced more new shoots as well as a higher number of leaves and length of new shoots on the medium supplemented with 1.5 mg × l-1 BA as compared with those from terminal buds. Significant differences were observed at multiplication stage between the lateral buds and terminal buds, since the lateral buds produced a higher number of new shoots and leaves as well as longer new shoots. At rooting stage, the treatment with 1 mg × l-1 IBA gave the highest percentage of rooting (100%), the highest number of roots (13.2 root/explant), and the longest roots (8.44 cm), respectively, on half strength MS medium. Plantlets obtained were transferred to pots and acclimatized with 90% success.

Phytoplankton of Samarra Reservoir [Iraq]

51%

Al-Lami A. A., Sabri A. W., Kassim T. I., Rasheed K. A.

Acta Hydrobiologica

1996

tom 38

nr 3-4

The phytoplankton community in three stations at Samarra Reservoir was studied seasonally. A total of 149 taxa were identified, ten of which were new additions to the Iraqi flora. The density ranged from 0.43 to 18.56 10³ ind. cm-³, Bacillariophyceae were found to be the dominant group. Variation in species dominance between seasons and stations was observed. The density of phytoplankton, chlorophyll ɑ concentration, and the share of Cyanophyta and Euglenophyta were higher at the station polluted with municipal sewage.

Ograniczanie wyników

1 Acta Agrobotanica

1 Acta Hydrobiologica

2 Rasheed K. A.

1 Al-Lami A. A.

1 Kassim T. I.

1 Sabri A. W.

1 2013

1 1996

Wyniki wyszukiwania

Improving an in vitro propagation protocol for Cestrum nocturnum L.

Phytoplankton of Samarra Reservoir [Iraq]