Tomato torrado virus is a member of Secoviridae family, genus Torradovirus. It is a dangerous pathogen of tomato plants causing intensive necrosis of leaves and fruits, leading to plant death, and significantly decreasing production of this vegetable. In Poland three isolates of ToTV were identified: Wal’03, Kra and Ros. On the basis of the previously performed molecular and genomic analyses distinctive genetic variability was revealed within 3`UTR region of RNA1 Kra and Wal’03 isolates. On the basis of this heterogenous region a rapid protocol of PCR-HRM reaction was developed allowing the identification and differentiation of two isolates of Tomato torrado virus as well as constituting rapid test to monitor the nucleotide point mutation within this regulatory region. Since the 3`UTR region is known to play a role in the replication process hence the featured heterogeneity might have an impact on the control of viral particles accumulation.
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