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The aim of our study was to observe the contents of nitrates and nitrites during the six stages of manufacture of Edam cheese. Samples were taken of raw milk, of pasteurized milk, of milk with nitrates added, of pressed cheese curd, of whey, of ripening cheese and the final product. The samples were drawn from a commercial operation in cheese factory in the eastern part of Slovakia. The mean natura NaNO₂ content in raw and pasteurized milk was 0.10 and 0.13 mg/kg, respectively. The mean natural NaNO₃ content in raw and pasteurized milk was 0.60 and 0.66 mg/kg, respectively. In milk with nitrate added the mean content was 96.58 mg/kg NaNO₃; the maximum value being 105.54 mg/kg NaNO₃. After pressing, the mean value of nitrate was found to be 15.45 mg/kg NaNO₃. A considerable quantity of nitrates passed into the whey, where the mean nitrate content was 76.58 mg/kg NaNO₃. The final product had a markedly decreased content of nitrates (1.69 mg/kg NaNO₃) and nitrites (0.61 mg/kg NaNO₂) when compared with the values in cheese during maturation (7.92 mg/kg NaNO₃ and 1.69 mg/kg NaNO₂).
Effect of both vitamin D₃ and cadmium (Cd) on chymotrypsin activity (ACHT) of laying hen has been studied. Ten month-old laying hens were fed a compound feed of HYD-10 ad libitum. The birds were divided into 8 groups (each group consisting of 6 birds) as follows: experimental groups G1, G3, and G5 were supplemented with Cd (0.3; 0.6; and 6.0 mg.kg⁻¹ CdCl₂, respectively) groups G2, G4, and G6 were supplemented with vitamin D₃ (100 I.U./day) at the same Cd concentrations. C₁ was control group without any supplements and C₂ was control group supplemented only with vitamin D₃. The study lasted 6 months. In groups G1-G4, ACHT activities in the end of the study were rather high (2097.4; 2984; 1805.4, and 2575.9 U.g⁻¹,respectively). The ACHT activity in groups G2, G4 and G6 was by little higher than that in groups G1, G3, and G5 without vitamin D₃ supplementation. The principle of the action of vitamin D₃ via Ca ions and calmodulin is further discussed.
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