Wyniki wyszukiwania

1

Rozwój diagnostyki molekularnej w medycynie

100%

Lubinski J.

Biotechnologia. Monografie

|

2006

|

nr 03

2

Hybrid finite element method development for offshore structures' calculation with the implementation of industry standards

63%

Lubinski J., Olszewski H.

Polish Maritime Research

|

2019

|

tom 26

|

nr 4

In the design process of offshore steel structures, it is typical to employ commercial calculation codes in which simulation and evaluation of results are performed on the basis of the available standards (e.g. API, DNV, Lloyds). The modeling and solution rely on finite element methods and cover the simulation of the structure’s properties along with the influence of the marine environment – sea currents, wave and wind loading, as well as the influence of vibrations, buoyancy and accompanying mass of water. Both commercial and open source mathematical modeling software which is available nowadays allows for cost effective and flexible implementation of advanced models for offshore industrial structures with high level of credibility and safety. The models can be built to suit task-specific requirements and evaluated on the basis of the selected criterial system best suited to the needs of the customer. Examples of methodology for environmental and structural model development are presented, along with simulation results covering a wide scope of data, ranging from stress and deformation to resonant characteristics and issues of technological feasibility

3

Transmission electron microscopy in the differential diagnosis of malignant cells in aspirates

63%

Lubinski J., Domagala W.

Folia Histochemica et Cytobiologica

|

1986

|

tom 24

|

nr 4

4

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Incidence of hereditary non-polyposis colorectal cancer [HNPCC] in the city of Szczecin, north-western Poland

63%

Kladny J., Lubinski J.

Journal of Applied Genetics

|

1997

|

tom 38

|

nr 1

The study population consisted of 140 consecutive colorectal cancer patients, inhabitants of the city of Szczecin, north-west Poland, who were histopathologically diagnosed in the period of 2 years - 1991-1992. Family history was obtained in 124 (88.6%) of patients. A definitive diagnosis of HNPCC was established if requirements of the International Collaborative Group on HNPCC (ICG- HNPCC) were met. Suspected HNPCC were recognised according to criteria described by Ponz de Leon or Mecklin or Kunitomo. HNPCC as defined by International Collaborative Group on HNPCC was identified in 2 (1.6%) families. Suspected HNPCC were recognised in 16.9%, 3.2% and 4.0% of patients if Ponz de Leon or Mecklin or Kunitomo criteria were applied, respectively. In our series in 19 of 124 cases, colorectal carcinomas were diagnosed in patients under 50 years of age. Only in one of these cases, features characteristic of HNPCC other than young age were found which suggests that in our region the frequency of somatic or germ line de novo mutations in genes predisposing to colorectal cancer may be high. Our results suggest that the frequency of HNPCC inherited from ancestors in Poland and other countries is approximately similar and this syndrome is common disease everywhere.

5

Immunocytochemical studies of clonality in cytologic material from lyphoproliferative disorders

51%

Podolski J., Chosia M., Lubinski J.

Folia Histochemica et Cytobiologica

|

1990

|

tom 28

|

nr 3

6

Ultrastructure of epithelial cells in rat's epididymal caput in experimental hyperprolactinemia induced by metoclopramide

51%

Laszczynska M., Rozewicka L., Lubinski J.

Folia Histochemica et Cytobiologica

|

1989

|

tom 27

|

nr 4

7

Age at diagnosis of cancer as predictor of mutation accurrence in families suspected of HNPCC

51%

Kurzawski G., Debniak T., Kladny J., Lubinski J.

Journal of Applied Genetics

|

2001

|

tom 42

|

nr 3

8

Increased constitutional chromosome sensitivity to bleomycin in patients with hereditary non-polyposis colorectal cancer [HNPCC]

51%

Kladny J., Zajaczek S., Lubinski J.

Journal of Applied Genetics

|

1996

|

tom 37

|

nr 4

It has been suggested that mutagen sensitivity is a constitutional factor which may be useful in identification of patients with an increased risk for the development of tumors. In this study, the chromosome sensitivity to bleomycin was measured according to Hsu in patients with hereditary non-polyposis colorectal cancer (HNPCC), sporadic colorectal cancer and in control persons with no tumor history in family. In vitro lymphocytes were exposed to bleomycin according to Hsu and chromosomal damage was quantified by scoring breaks of 100 cells. A significant difference (P < 0.01) in the mean number of breaks per cell (b/c) was found between HNPCC patients (0.59 ± 0.14; n = 12; mean age 55.4 yrs) and control individuals (0.35 ± 0.13: n = 12; mean age 55.8 yrs). In contrast, patients with sporadic colorectal cancer showed a mean b/c value of 0.43 ± 0.14 (n = 14; mean age 63.4 yrs) which was not significantly higher than that in control individuals for this group (0.42 ± 0.15; n = 14; mean age 63.1 yrs). Selenium protected lymphocytes of HNPCC patients against bleomycin activity in vitro.

9

Constitutional chromosome instability in families with patients affected by sporadic non-hereditary retinoblastoma

51%

Zajaczek S., Gorski B., Krzystolik Z., Lubinski J.

Journal of Applied Genetics

|

1999

|

tom 40

|

nr 4

10

Antigens HLA-G, sHLA-G and sHLA- class I in reproductive failure

45%

Sipak-Szmigiel O., Ronin-Walknowska E., Cybulski C., Plonka T., Lubinski J.

Folia Histochemica et Cytobiologica. Supplement

|

2007

|

tom 45

|

nr 1

137-141

11

Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD[P]H: quinone reductase and apoptosis in human lymphoblastoid cells

45%

Misiewicz I., Skupinska K., Kowalska E., Lubinski J., Kasprzycka-Guttman T.

Acta Biochimica Polonica

|

2004

|

tom 51

|

nr 3

The effect of sulforaphane on human lymphoblastoid cells originating from a patient of a high cancer risk was studied. Sulforaphane (SFN) is a naturally occurring substance of chemopreventive activity. In our study, changes in cell growth, induction of apoptosis and phase 2 enzymes as well as glutathione level were examined. Apoptosis was tested by confocal microscopy at three stages: change in mitochondrial membrane potential, caspase activation and phosphatidylserine externalization. We show that SFN increases the activity of the detoxification system: it increases quinone reductase activity at low concentration (0.5-1 uM) and raises glutathione level in a dose-dependent manner. At higher doses (2.5-10 uM) sulforaphane is a cell growth modulator, as it caused cell growth cessation (IC50 = 3.875 uM), and apoptosis inducer. The results obtained suggest that sulforaphane acts as a chemopreventive agent in human lymphoblastoid cells.

12

The first Rb-1 gene promoter germ-line de novo mutation in patient with retinoblastoma

45%

Zajaczek S., Jakubowska A., Kurzawski G., Krzystolik Z., Lubinski J.

Journal of Applied Genetics

|

1999

|

tom 40

|

nr 3

13

Frequency of mutations related to hereditary haemochromatosis in northwestern Poland

39%

Raszeja-Wyszomirska J., Kurzawski G., Suchy J., Zawada I., Lubinski J., Milkiewicz P.

Journal of Applied Genetics

|

2008

|

tom 49

|

nr 1

105-107

Hereditary haemochromatosis has been linked with C282Y and H63D mutations of the HFE gene. In Europe, frequencies of these mutations are the highest in Northern European countries and gradually decrease southwards. We analysed the prevalence of HFE mutations in 1517 DNA samples, including 1000 samples from the general population (subjects registered at general practitioner practices) in northwestern Poland, and 517 samples of cord blood from the same region. We identified 2 (0.13%) homozygotes and 117 (7.8%) heterozygotes for the C282Y mutation. As regards the H63D mutation (1505 DNA samples analysed), 38 (2.5%) samples were homozygotes and 380 (25%) were heterozygotes. Twenty-one (1.4%) compound heterozygotes were found. These results correspond well with data from other Central European countries and seem to confirm the hypothesis of North-South spread of the C282Y mutation.

14

Molecular analysis of a large novel constitutional deletion in a patient with sporadic unilateral retinoblastoma

39%

Zajaczek S., Cragg H., Jakubowska A., Gorski B., Krzystolik Z., Cowell J. K., Lubinski J.

Journal of Applied Genetics

|

1999

|

tom 40

|

nr 3

15

Non-isotopic procedure with silver staining of polyacrylamide gels in detection of genomic abnormalities in tumors using microsatellites

33%

Podolski J., Hadaczek P., Gibas Z., Gibas L., Grossman H. B., Limon J., Huebner K., Lubinski J.

Folia Histochemica et Cytobiologica

|

1995

|

tom 33

|

nr 2

16

Usefulness of polymorphic markers in exclusion of BRCA1-BRCA2 mutations in families with aggregation of breast ovarian cancers

33%

Gorski B., Debniak T., Jakubowska A., Cybulski C., Huzarski T., Byrski T., Zlowocka E., Lubinski J.

Journal of Applied Genetics

|

2003

|

tom 44

|

nr 3

17

Molecular basis of inherited predispositions for tumors

33%

Lubinski J., Gorski B., Kurzawski G., Jakubowska A., Cybulski C., Suchy J., Debniak T., Grabowska E., Lener M., Nej K.

Acta Biochimica Polonica

|

2002

|

tom 49

|

nr 3

On the basis of literature data and own experience the authors review the current knowledge about the molecular basis of inherited predispositions for tumors. They hypothesize that in the near perspective 5-10 years studies using existing registry data/material and the latest novel technology will allow the identification of the molecular background for the majority of hereditary cancers which will have enormous practical consequences especially for the prevention of malignancies.

18

Ovarian cystadenoma as a characteristics feature of families with hereditary ovarian cancers unassociated with BRCA1 and BRCA2 mutations

27%

Menkiszak J., Brzosko M., Gorski B., Flicinski J., Jakubowska A., Zebielowicz D., Gronwald J., Huzarski T., Byrski T., Teresinski L., Chosia M., Rzepka-Gorska I., Lubinski J.

Journal of Applied Genetics

|

2004

|

tom 45

|

nr 2

19

Hereditary tumors - prophylactics, early diagnosis, treatment

27%

Lubinski J., Zajaczek S., Kladny J., Kurzawski G., Menkiszak J., Hadaczek P., Cybulski C., Krzystolik K., Toloczko A., Podolski J., Gronwald J.

Biotechnologia

|

1996

|

nr 4

Ograniczanie wyników

Rozwój diagnostyki molekularnej w medycynie

Hybrid finite element method development for offshore structures' calculation with the implementation of industry standards

Transmission electron microscopy in the differential diagnosis of malignant cells in aspirates

Incidence of hereditary non-polyposis colorectal cancer [HNPCC] in the city of Szczecin, north-western Poland

Immunocytochemical studies of clonality in cytologic material from lyphoproliferative disorders

Ultrastructure of epithelial cells in rat's epididymal caput in experimental hyperprolactinemia induced by metoclopramide

Age at diagnosis of cancer as predictor of mutation accurrence in families suspected of HNPCC

Increased constitutional chromosome sensitivity to bleomycin in patients with hereditary non-polyposis colorectal cancer [HNPCC]

Constitutional chromosome instability in families with patients affected by sporadic non-hereditary retinoblastoma

Antigens HLA-G, sHLA-G and sHLA- class I in reproductive failure

Sulforaphane-mediated induction of a phase 2 detoxifying enzyme NAD[P]H: quinone reductase and apoptosis in human lymphoblastoid cells

The first Rb-1 gene promoter germ-line de novo mutation in patient with retinoblastoma

Frequency of mutations related to hereditary haemochromatosis in northwestern Poland

Molecular analysis of a large novel constitutional deletion in a patient with sporadic unilateral retinoblastoma

Non-isotopic procedure with silver staining of polyacrylamide gels in detection of genomic abnormalities in tumors using microsatellites

Usefulness of polymorphic markers in exclusion of BRCA1-BRCA2 mutations in families with aggregation of breast ovarian cancers

Molecular basis of inherited predispositions for tumors

Ovarian cystadenoma as a characteristics feature of families with hereditary ovarian cancers unassociated with BRCA1 and BRCA2 mutations

Hereditary tumors - prophylactics, early diagnosis, treatment