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Determinaton of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs by liquid chromatography with fluorescence detection

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Gdybik-Sikorska M., Posyniak A., Gajda A., Bladek T.

Bulletin of the Veterinary Institute in Puławy

2013

tom 57

nr 3

The study presents a method for determination of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs with the use of liquid chromatography with fluorescence detection. The procedure enables a simple isolation of fluoroquinolones from albumin based on extraction with acetonitrile, and from freeze-dried-eggs with acetonitrile under alkaline conditions. The samples have been analysed on ultracarb C8 liquid chromatography column. The gradient elution programmes consisted of the mixture of 0.03 M phosphoric acid with 0.002 M sodium 1-heptanesulfonate monohydrate and acetonitrile. The method has been validated according to requirements of the European Decision 2002/657/EC. Recoveries ranged from 78% to 83% for spiked freeze-dried-eggs and from 89% to 91% for albumin. The developed method can be applied for determination and confirmation of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Bladek T.

1 Gajda A.

1 Gdybik-Sikorska M.

1 Posyniak A.

1 2013

Wyniki wyszukiwania

Determinaton of enrofloxacin and ciprofloxacin in albumin and freeze-dried-eggs by liquid chromatography with fluorescence detection