We carried out propagation of lactic acid bacteria, using different process parameters: a) concentration of wort extract in the range of 8–16 °Plato; b) temperature 25°C and 37°C. The number of bacteria, their viability and a pH value of the growth medium were measured. The aim of the study was to identify the best parameters of the propagation process, which would allow to obtain in a short time a suspension containing bacteria of high vitality and abundance. On the basis of the results the wort of 12°Plato was proposed to use for propagation of lactic acid bacteria. Process temperature, especially for propagation lasting more than 24 h, should be relatively low (25°C). Application of higher temperature (37°C) results in good propagation after 1 day, but the bacterial viability decreases considerably when the process is prolonged (after 7 days the dead cell count was 73% (compared to 1.3% after 1 day). If the temperature is lower, the drop in viability is not so dramatic (after 7 days the dead cell count was 42%).